HISTOCHEMICAL DEMONSTRATION OF DEHYDROGENASE ACTIVITY IN THE CELLS OF NORMAL HUMAN BLOOD AND BONE MARROW

Endogenous and succinic dehydrogenase activity was demonstrated in the living cells of normal human blood and bone marrow using a buffered nitro BT-succinate incubating solution. With this technique dehydrogenase activity was localized primarily in the granular leukocytes and the sites of enzymatic activity appeared to be non-mitochondrial. The addition of a non-ionic surface active agent to the incubating solution resulted in marked differences in the cellular and intracellular localization of dehydrogenase activity. With this method it was possible to demonstrate dehydrogenase activity in the mitochondria of most of the formed elements of the blood and bone marrow, including developing granulocytes and erythroid cells, agranulocytes, and blood platelets. Mature erythrocytes also exhibited a minimal dehydrogenase reaction with this procedure. This investigation indicated that in order adequately to demonstrate and evaluate dehydrogenase activity in the cells of the blood and bone marrow it was necessary to have increased cellular and mitochondrial permeability, as well as partially viable cells with an intact dehydrogenase system.

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Measurement of ionized calcium in normal human blood platelets

Analytical Biochemistry ◽

10.1016/0003-2697(88)90303-x ◽

1988 ◽

Vol 169 (2) ◽

pp. 400-404 ◽

Cited By ~ 33

Author(s):

Gundu H.R. Rao

Keyword(s):

Human Blood ◽

Blood Platelets ◽

Ionized Calcium ◽

Normal Human ◽

Human Blood Platelets ◽

Normal Human Blood

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The Significance of Malate Dehydrogenase Isoenzymes in Human Blood Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647886 ◽

1975 ◽

Vol 33 (02) ◽

pp. 341-353

Author(s):

W Schneider ◽

R Gross

Keyword(s):

Malate Dehydrogenase ◽

Human Blood ◽

Mitochondrial Membrane ◽

Blood Platelets ◽

Creatine Phosphate ◽

Transfer System ◽

Malate Transport ◽

Normal Human ◽

Human Blood Platelets ◽

Normal Human Blood

SummaryTwo MDH isoenzymes were detected in the homogenate of normal human blood platelets. According to their properties the cationic isoenzyme is compartmentalized in the mitochondria, the anionic one belongs to the cytoplasma. In spite of the few mitochondria in human blood platelets the proportion of the cationic enzyme is relatively high.Both of these enzymes could belong to a transfer system for malate transport across the mitochondrial membrane. As human blood platelets do not contain creatine phosphate a system like that could be of significance for platelet function.

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A Study of the Morphology of the Living Cells of Blood and Bone Marrow in Vital Films with the Phase Contrast Microscope

Blood ◽

10.1182/blood.v10.1.3.3 ◽

1955 ◽

Vol 10 (1) ◽

pp. 3-16 ◽

Cited By ~ 21

Author(s):

G. ADOLPH ACKERMAN ◽

NICHOLAS C. BELLIOS

Keyword(s):

Bone Marrow ◽

Human Blood ◽

Phase Contrast ◽

Living Cells ◽

Living Condition ◽

Phase Contrast Microscope ◽

Morphologic Characteristics ◽

Contrast Microscope ◽

Normal Human ◽

Normal Human Blood

Abstract The cells of normal human blood and bone marrow have been examined in the living condition by means of the phase contrast microscope employing both supravital and unstained most films. The morphologic characteristics of the cells studied in this manner have been carefully described and illustrated.

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Acid Phosphatase Activity in Normal Human Blood and Bone Marrow Cells as Demonstrated by the Azo Dye Method

Acta Haematologica ◽

10.1159/000208138 ◽

1963 ◽

Vol 30 (5) ◽

pp. 310-316 ◽

Cited By ~ 21

Author(s):

L. Rozenszajn ◽

G. Marshak ◽

P. Efrati

Keyword(s):

Bone Marrow ◽

Acid Phosphatase ◽

Phosphatase Activity ◽

Human Blood ◽

Acid Phosphatase Activity ◽

Azo Dye ◽

Bone Marrow Cells ◽

Normal Human ◽

Normal Human Blood ◽

Marrow Cells

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A Method for Separating and Concentrating Platelets from Normal Human Blood

Blood ◽

10.1182/blood.v7.7.693.693 ◽

1952 ◽

Vol 7 (7) ◽

pp. 693-699 ◽

Cited By ~ 36

Author(s):

ALLEN H. MINOR ◽

LEE BURNETT

Keyword(s):

Human Blood ◽

Surface Active Agent ◽

Active Agent ◽

Platelet Concentrates ◽

Clot Retraction ◽

Physiological Fluid ◽

Normal Human ◽

Surface Active ◽

Hemostatic Effects ◽

Normal Human Blood

Abstract A method is described for the preparation of platelet concentrates from pint volumes of normal human blood collected in standard ACD bottles. Erythrocytes are removed by sedimentation with high molecular weight dextran, and platelets are concentrated by centrifugation in the presence of a surface-active agent, which permits their resuspension in a small volume of physiological fluid following prolonged centrifugation. The procedure results in the recovery of about 80 per cent of the total platelet complement of the blood. The platelets obtained are discrete and morphologically intact, and induce normal prothrombin consumption and clot retraction when added to platelet-poor plasma or thrombocytopenic blood. Transfusions of platelet concentrates given to thrombocytopenic patients have been followed by hemostatic effects lasting from 1 to 5 days.

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Comparison of Normal and Thrombasthenic Human Blood Platelets

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100731 ◽

1968 ◽

Vol 26 ◽

pp. 198-199

Author(s):

N. F. Rodman ◽

J. C. Painter ◽

R. G. Mason

Keyword(s):

Human Blood ◽

Resting State ◽

Blood Platelets ◽

Autologous Plasma ◽

Marginal Band ◽

Normal Human ◽

Similar Appearance ◽

Human Blood Platelets ◽

Morphologic Changes ◽

Normal Human Blood

The ultrastructure of normal human blood platelets in the resting state has been described in detail. Platelets recovered from citrated plasma of a patient with Glanzmann's thrombopathy or thrombasthenia have a similar appearance (Fig. 1). These anucleate cells are usually disc shaped and hence are round or oval, depending upon the plane of sectioning. They have randomly dispersed cytoplasmic organelles and microtubules which are typically arranged in a marginal band.Platelet responses to several agglutinating agents and the relation of platelets to fibrin during clotting have been reported. On exposure to thrombin, to ADP, or to collagen, normal and thrombasthenic platelets undergo similar early morphologic changes. Pseudopods form and organelles move toward platelet centers, with microtubules being outermost of the closely packed, centrally apposed organelles. A peripheral rim of cytoplasm is essentially organelle-free except for an occasional mitochondrion or vacuole and except for microtubules which sometimes extend outward into pseudopods. Following these early, preagglutination changes normal platelets agglutinate in response to each of the 3 agglutinating agents, whether in autologous plasma or in salt solution after repeated washings. Thrombasthenic platelets in autologous plasma fail to agglutinate in response to thrombin or to ADP and agglutinate only minimally on exposure to finely divided collagen.

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