scholarly journals IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN CHORIONIC GONADOTROPIN

1962 ◽  
Vol 115 (2) ◽  
pp. 289-294 ◽  
Author(s):  
A. R. Midgley ◽  
G. B. Pierce
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Hydatidiform Mole ◽  
Immunohistochemical Localization ◽  
Formalin Fixation ◽  
Cell Of Origin ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Immunohistochemical Techniques ◽  
Formalin Fixed

Through the use of immunohistochemical techniques, human chorionic gonadotropin has been localized to syncytiotrophoblastic cells of immature placenta, hydatidiform mole, chorioadenoma destruens, and choriocarcinoma. No gonadotropin has been detected in cytotrophoblast. Evidence is discussed which suggests that syncytiotrophoblast is the cell of origin of human chorionic gonadotropin. The observation that formalin fixation did not alter the ability of human chorionic gonadotropin to react with its specific antibody permitted the study of formalin-fixed paraffin-embedded tissues stored in the tissue collection. In addition, the excellence of histologic preparations following formalin fixation facilitated cytologic identification.

scholarly journals Determining cell-of-origin subtypes of diffuse large B-cell lymphoma using gene expression in formalin-fixed paraffin-embedded tissue

Blood ◽  
2014 ◽  
Vol 123 (8) ◽  
pp. 1214-1217 ◽  
Author(s):  
David W. Scott ◽  
George W. Wright ◽  
P. Mickey Williams ◽  
Chih-Jian Lih ◽  
William Walsh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Cell Of Origin ◽  
Large B Cell Lymphoma ◽  
Formalin Fixed Paraffin ◽  
Key Points ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Large B Cell

Key Points A 20-gene gene expression-based assay accurately and robustly assigns COO subtypes of DLBCL using formalin-fixed paraffin-embedded tissue.

scholarly journals Immunohistochemical detection of thrombospondin-1 in formalin-fixed, paraffin-embedded tissue.

1996 ◽  
Vol 44 (7) ◽  
pp. 761-766 ◽  
Author(s):  
G D Grossfeld ◽  
S R Shi ◽  
D A Ginsberg ◽  
K A Rich ◽  
D G Skinner ◽  
...  
Keyword(s):  
Transitional Cell ◽  
Antigen Retrieval ◽  
Cultured Fibroblasts ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Thrombospondin 1 ◽  
Formalin Fixed Paraffin Embedded ◽  
Carcinoma Of The Bladder ◽  
Immunohistochemical Techniques ◽  
Formalin Fixed

Thrombospondin-1 (TSP) is a 450-KD glycoprotein that was initially discovered in the platelet alpha-granule. It now appears that TSP is intimately involved in the regulation of a variety of cellular functions and cell-to-cell interactions. Recently, it has been demonstrated that TSP functions as a p53-dependent inhibitor of angiogenesis in cultured fibroblasts from Li-Fraumeni patients and therefore may be an important factor involved with tumor invasion and metastasis. It has previously been demonstrated that TSP can be detected in frozen tissue sections by immunohistochemical methods. Our objective in this study was to determine the optimal antigen retrieval (AR) protocol for detection of TSP in formalin-fixed, paraffin-embedded tissue by using tissue sections from patients with invasive transitional cell carcinoma of the bladder. The optimal AR protocol was determined utilizing a variety of heating conditions and antigen retrieval buffers. Our results demonstrate that TSP can be reliably detected in paraffin-embedded tissue by immunohistochemical techniques that utilize AR with high-temperature microwave heating and a low-pH Tris-HCI buffer. The importance of this method is that it allows the reliable detection of TSP in archival tissue. This should facilitate further investigation into TSP's role in the regulation of cellular processes, including its influence on tumor angiogenesis and metastasis.

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