scholarly journals COMPLEMENT FIXATION BY A TWO-COMPONENT ANTIBODY SYSTEM: IMMUNOGLOBULIN G AND IMMUNOGLOBULIN M ANTI-GLOBULIN (RHEUMATOID FACTOR)

1970 ◽  
Vol 132 (4) ◽  
pp. 673-693 ◽  
Author(s):  
Frank R. Schmid ◽  
Ivan M. Roitt ◽  
Maria J. Rocha
Keyword(s):  
Rheumatoid Factor ◽  
Complement Fixation ◽  
Direct Interaction ◽  
Fluid Phase ◽  
Kinetic Studies ◽  
Immunoglobulin M ◽  
Inhibitory Potency ◽  
Cell Agglutination ◽  
Igm Rheumatoid Factor ◽  
Rabbit Igg

Complement-mediated lysis of sheep erythrocytes coated with optimal concentrations of rabbit IgG hemolysin was inhibited by euglobulin fractions from the sera of patients with seropositive rheumatoid arthritis. That this was due to direct interaction with the IgG coat on the red cell rather than a nonspecific reaction with complement in the fluid phase was confirmed by controls using cells coated with IgM hemolysin. The inhibitory activity was recovered in purified IgM rheumatoid factor preparations and could be absorbed out with insoluble aggregated human IgG. The inhibitory potency of the rheumatoid factors correlated well with their sheep cell agglutination titers. Inhibition was not the result of physical aggregation of the erythrocytes by rheumatoid factor. Kinetic studies were consistent with the view that rheumatoid factor displaces C1q from its binding to IgG. Paradoxically, at suboptimal sensitizing concentrations of IgG hemolysin, rheumatoid factor enhances the fixation of complement. These results can be interpreted on the basis of the blockage of complement fixation by IgG and its replacement by a relatively weak direct fixation by the IgM rheumatoid factor. Thus, the interaction of RF with IgG generates only a limited ability to fix complement which, when contrasted with the fixation at suboptimal concentrations of IgG hemolysin alone, appears as net enhancement; when this is contrasted with fixation occurring with optimal concentrations of IgG, it appears as net inhibition.

scholarly journals Role of rheumatoid factor in complement fixation and indirect hemagglutination tests for immunoglobulin M antibody to cytomegalovirus

1978 ◽  
Vol 8 (2) ◽  
pp. 160-165
Author(s):  
N E Cremer ◽  
M Hoffman ◽  
E H Lennette
Keyword(s):  
Rheumatoid Factor ◽  
Antibody Titer ◽  
Gamma Globulin ◽  
Complement Fixation ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
Igg Antibody ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test

Absorption of immunoglobulin M (IgM)-rheumatoid factor (RF) from serum samples by reaction with insolubilized gamma globulin reduced the complement-fixing (CF) antibody titer to cytomegalovirus (CMV) antigen to less than 1:2 in the IgM fraction of some, but not all, sera. Thus, IgM-CF activity in some sera appeared to be due to specific IgM anti-CMV antibody and in other sera to complexes of IgM-RF with antiviral IgG antibody. Prozones were present in the CF tests on IgM fractions. Increasing the concentration of antigen from 2 to 4 U reduced the prozone titer by one or two double dilutions. This observation suggested that a competition for antigen may be operating at low dilutions of IgM antibody fractions. Removal of RF had little or no effect on the reaction of the IgM fraction of sera with CMV by the indirect hemagglutination test.

scholarly journals Rheumatoid factor: a cause of fals positive histoplasmin latex agglutination

1977 ◽  
Vol 5 (1) ◽  
pp. 31-33
Author(s):  
R W Oxenhandler ◽  
E H Adelstein ◽  
W A Rogers
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
False Positive ◽  
Complement Fixation ◽  
High Titer ◽  
Latex Agglutination ◽  
Immunoglobulin M ◽  
Cold Agglutinins ◽  
Clinical Records

Ten of thirteen patients with positive histolatex agglutination titers of 1:32 or greater had no evidence of acute histoplasmosis.Three of these false positives had rheumatoid arthritis. A fourth had a rising mycoplasma complement fixation titer, and the fifth had a high titer of cold agglutinins. All of these are associated with abnormal immunoglobulin M production. To evaluate the role of rheumatoid factor in producing false positive histolatex agglutination, the histolatex test was performed on sera from 32 patients having rheumatoid factor at a titer of 1:40 or greater. Four of these sera agglutinated the histoplasmin-coated latex particles at titers of 1:32 or greater. Review of clinical records suggests the this reactivity is nonspecific. It is our purpose to call attention to rheumatoic factor as a cause of false positive histolatex agglutination.

scholarly journals Early Antibody Responses to Experimental Mycobacterium bovis Infection of Cattle

2006 ◽  
Vol 13 (6) ◽  
pp. 648-654 ◽  
Author(s):  
W. R. Waters ◽  
M. V. Palmer ◽  
T. C. Thacker ◽  
J. P. Bannantine ◽  
H. M. Vordermeier ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Skin Testing ◽  
Rapid Test ◽  
Kinetic Studies ◽  
Immunoblot Analysis ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
Mycobacterial Antigens ◽  
New Generation ◽  
And Control

ABSTRACT Bovine tuberculosis persists as a costly zoonotic disease in numerous countries despite extensive eradication and control efforts. Sequential serum samples obtained from Mycobacterium bovis-infected cattle were evaluated for seroreactivity to mycobacterial antigens. Animals received M. bovis by aerosol, intratonsil, intranasal, or intratracheal inoculation. Assays included the multiantigen print immunoassay for determination of antigen recognition patterns, immunoblot analysis for sensitive kinetic studies, and the VetTB STAT-PAK test, a novel, rapid test based on lateral-flow technology. Responses to MPB83 were detected for all M. bovis-infected animals regardless of the route or strain of M. bovis used for inoculation. Other less commonly recognized antigens included ESAT-6, CFP-10, and MPB70. Responses to MPB83 were detectable as early as 4 weeks after inoculation, were boosted upon injection of purified protein derivatives for skin testing, and persisted throughout the course of each of the four challenge studies. MPB83-specific immunoglobulin M (IgM) was detected prior to MPB83-specific IgG detection; however, early IgM responses rapidly waned, suggesting a benefit of tests that detect both IgM- and IgG-specific antibodies. The VetTB STAT-PAK test detected responses in sera from 60% (15/25) of the animals by 7 weeks after challenge and detected responses in 96% (24/25) of the animals by 18 weeks. These findings demonstrate the potential for new-generation antibody-based tests for the early detection of M. bovis infection in cattle.

Difference between conformations of immunoglobulin M and human rheumatoid factor based on small-angle X-ray solution scattering data

2013 ◽  
Vol 23 (6) ◽  
pp. 319-320
Author(s):  
Vladimir V. Volkov ◽  
Victor A. Lapuk ◽  
Deniza I. Chekrygina ◽  
Elena Yu. Varlamova ◽  
Artem V. Chekushin
Keyword(s):  
Rheumatoid Factor ◽  
Small Angle ◽  
Immunoglobulin M ◽  
Scattering Data ◽  
X Ray

scholarly journals Estimation of the relative avidity of 19S IgM rheumatoid factor secreted by rheumatoid synovial cells for human IgG subclasses

1986 ◽  
Vol 29 (6) ◽  
pp. 722-729 ◽  
Author(s):  
Dick L. Robbins ◽  
Jeffrey Skilling ◽  
William F. Benisek ◽  
Richard Wistar
Keyword(s):  
Rheumatoid Factor ◽  
Igg Subclasses ◽  
Synovial Cells ◽  
Human Igg ◽  
Igm Rheumatoid Factor
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