scholarly journals B cell repertoire diversity in athymic mice.

1980 ◽  
Vol 151 (3) ◽  
pp. 761-766 ◽  
Author(s):  
M P Cancro ◽  
N R Klinman
Keyword(s):  
B Cell ◽  
Pattern Analysis ◽  
Athymic Mice ◽  
Cell Repertoire ◽  
Reactivity Pattern ◽  
Influenza Hemagglutinin ◽  
Immunoglobulin Allotype ◽  
B Cell Repertoire ◽  
Repertoire Diversity

The extent of B cell repertoire diversity among nu/nu BALB/c mice has been assessed and compared with that of normal BALB/c mice. This was accomplished through the characterization of monoclonal, influenza hemagglutinin-specific antibodies by reactivity pattern analysis. The results indicate that the repertoire of athymic mice is equivalent in diversity to that of normal mice. Moreover, because these responses were obtained in recipients that were histocompatible but distinct at immunoglobulin allotype loci, these findings indicate that a very diverse array of B cell clonotypes may be stimulated in the absence of allotype-identical T cells.

scholarly journals Characterization of T and B cell repertoire diversity in patients with RAG deficiency

2016 ◽  
Vol 1 (6) ◽  
pp. eaah6109-eaah6109 ◽  
Author(s):  
Y. N. Lee ◽  
F. Frugoni ◽  
K. Dobbs ◽  
I. Tirosh ◽  
L. Du ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Repertoire Diversity ◽  
Rag Deficiency

scholarly journals Inferring processes underlying B-cell repertoire diversity

2015 ◽  
Vol 370 (1676) ◽  
pp. 20140243 ◽  
Author(s):  
Yuval Elhanati ◽  
Zachary Sethna ◽  
Quentin Marcou ◽  
Curtis G. Callan ◽  
Thierry Mora ◽  
...  
Keyword(s):  
B Cell ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Statistical Properties ◽  
Cell Repertoire ◽  
Vdj Recombination ◽  
Site Model ◽  
Human B Cells ◽  
B Cell Repertoire ◽  
Repertoire Diversity

We quantify the VDJ recombination and somatic hypermutation processes in human B cells using probabilistic inference methods on high-throughput DNA sequence repertoires of human B-cell receptor heavy chains. Our analysis captures the statistical properties of the naive repertoire, first after its initial generation via VDJ recombination and then after selection for functionality. We also infer statistical properties of the somatic hypermutation machinery (exclusive of subsequent effects of selection). Our main results are the following: the B-cell repertoire is substantially more diverse than T-cell repertoires, owing to longer junctional insertions; sequences that pass initial selection are distinguished by having a higher probability of being generated in a VDJ recombination event; somatic hypermutations have a non-uniform distribution along the V gene that is well explained by an independent site model for the sequence context around the hypermutation site.

scholarly journals Molecular characterization of the cervical and systemic B-cell repertoire

mAbs ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 181-191 ◽  
Author(s):  
Ryan G. Gaudet ◽  
Felix Breden ◽  
Frank Plummer ◽  
Jody D. Berry
Keyword(s):  
B Cell ◽  
Molecular Characterization ◽  
Cell Repertoire ◽  
B Cell Repertoire

scholarly journals Inferring processes underlying B-cell repertoire diversity.

2015 ◽  
Author(s):  
Yuval Elhanati ◽  
Zachary Sethna ◽  
Quentin Marcou ◽  
Curtis G Callan ◽  
Thierry Mora ◽  
...  
Keyword(s):  
B Cell ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Statistical Properties ◽  
Cell Repertoire ◽  
Vdj Recombination ◽  
Site Model ◽  
Human B Cells ◽  
B Cell Repertoire ◽  
Repertoire Diversity

We quantify the VDJ recombination and somatic hypermutation processes in human B-cells using probabilistic inference methods on high-throughput DNA sequence repertoires of human B-cell receptor heavy chains. Our analysis captures the statistical properties of the naive repertoire, first after its initial generation via VDJ recombination and then after selection for functionality. We also infer statistical properties of the somatic hypermutation machinery (exclusive of subsequent effects of selection). Our main results are the following: the B-cell repertoire is substantially more diverse than T-cell repertoires, due to longer junctional insertions; sequences that pass initial selection are distinguished by having a higher probability of being generated in a VDJ recombination event; somatic hypermutations have a non-uniform distribution along the V gene that is well explained by an independent site model for the sequence context around the hypermutation site.

scholarly journals Restricted adult clonal profiles induced by neonatal immunization. Influence of suppressor T cells.

1983 ◽  
Vol 158 (1) ◽  
pp. 112-125 ◽  
Author(s):  
M A Thompson ◽  
S Raychaudhuri ◽  
M P Cancro
Keyword(s):  
B Cell ◽  
Mechanistic Model ◽  
Strong Relationship ◽  
Specific Response ◽  
Suppressor T Cells ◽  
Cell Repertoire ◽  
Influenza Hemagglutinin ◽  
B Cell Repertoire ◽  
Apparent Loss ◽  
Original Antigenic Sin

The effects of neonatal antigen exposure on the adult B cell repertoire have been examined by characterizing the influenza hemagglutinin (HA)-specific response of adult BALB/c mice given antigen soon after birth. Ligand exposure during early life exerts a profound and lasting effect upon the B cell repertoire, characterized by the expansion and preservation of particular antigen-reactive clones and the apparent loss of others. The precise subset of clonotypes selectively preserved depends upon the age at which antigen is first encountered; and is predictable given a knowledge of the emerging primary pool's dynamics and composition. The preserved (secondary) B cells differ from their unprimed precursors with respect to (a) expression of the surface marker detected by the monoclonal antibody J11d, and (b) susceptibility to T cell-mediated suppression. These studies thus demonstrate a strong relationship between the heritable dynamics of the emerging primary B cell repertoire and the effect of ligand-driven events upon repertoire phenotype. In addition, they provide a mechanistic model for certain forms of antigen-induced oligoclonal dominance, especially the phenomenon of original antigenic sin.

scholarly journals Genetic background and immunological status influence B cell repertoire diversity in mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nancy Chaaya ◽  
Melody A. Shahsavarian ◽  
Irene Maffucci ◽  
Alain Friboulet ◽  
Bernard Offmann ◽  
...  
Keyword(s):  
B Cell ◽  
Genetic Background ◽  
High Throughput Sequencing ◽  
Immunoglobulin Gene ◽  
Cell Repertoire ◽  
Immunological Status ◽  
Complex Interactions ◽  
B Cell Repertoire ◽  
Scfv Library ◽  
Repertoire Diversity

Abstract The relationship between the immune repertoire and the physiopathological status of individuals is essential to apprehend the genesis and the evolution of numerous pathologies. Nevertheless, the methodological approaches to understand these complex interactions are challenging. We performed a study evaluating the diversity harbored by different immune repertoires as a function of their physiopathological status. In this study, we base our analysis on a murine scFv library previously described and representing four different immune repertoires: i) healthy and naïve, ii) healthy and immunized, iii) autoimmune prone and naïve, and iv) autoimmune prone and immunized. This library, 2.6 × 109 in size, is submitted to high throughput sequencing (Next Generation Sequencing, NGS) in order to analyze the gene subgroups encoding for immunoglobulins. A comparative study of the distribution of immunoglobulin gene subgroups present in the four libraries has revealed shifts in the B cell repertoire originating from differences in genetic background and immunological status of mice.

scholarly journals Hepatitis C Virus Epitope Immunodominance and B Cell Repertoire Diversity

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 983
Author(s):  
Nicholas A. Brasher ◽  
Anurag Adhikari ◽  
Andrew R. Lloyd ◽  
Nicodemus Tedla ◽  
Rowena A. Bull
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
B Cell ◽  
Rational Design ◽  
Current Knowledge ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Gene Usage ◽  
Repertoire Diversity ◽  
Virus Epitope

Despite the advent of effective, curative treatments for hepatitis C virus (HCV), a preventative vaccine remains essential for the global elimination of HCV. It is now clear that the induction of broadly neutralising antibodies (bNAbs) is essential for the rational design of such a vaccine. This review details the current understanding of epitopes on the HCV envelope, characterising the potency, breadth and immunodominance of antibodies induced against these epitopes, as well as describing the interactions between B-cell receptors and HCV infection, with a particular focus on bNAb heavy and light chain variable gene usage. Additionally, we consider the importance of a public repertoire for antibodies against HCV, compiling current knowledge and suggesting that further research in this area may be critical to the rational design of an effective HCV vaccine.

scholarly journals Assessing human B cell repertoire diversity and convergence

2018 ◽  
Vol 284 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Katharina Imkeller ◽  
Hedda Wardemann
Keyword(s):  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Repertoire Diversity ◽  
Human B Cell
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