scholarly journals Restriction fragment length polymorphism of the gene encoding the alpha chain of the human T cell receptor.

1985 ◽  
Vol 162 (3) ◽  
pp. 1087-1092 ◽  
Author(s):  
M L Hoover ◽  
J Marks ◽  
J Chipman ◽  
E Palmer ◽  
P Stastny ◽  
...  
Keyword(s):  
T Cell ◽  
Fragment Length Polymorphism ◽  
Antigen Receptor ◽  
Chain Gene ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Receptor Alpha ◽  
Cell Antigen ◽  
Alpha Chain ◽  
Receptor Alpha Chain

Two allelic forms of the T cell antigen receptor alpha chain gene were discerned by restriction fragment length polymorphism (RFLP) employing the T cell antigen receptor alpha chain probe pGA5, and the restriction enzyme Bgl II. Analysis revealed that the polymorphic fragments are detected by a probe specific for the constant region exon of the T cell antigen receptor alpha chain gene. Furthermore, the polymorphic fragments were shown to segregate within families. The two allelic forms yield two homozygous states, 3.2/3.2 and 2.9/2.9, at a frequency of 76.5 and 2.9%, respectively, within the normal population. The heterozygous state was observed in 20.6% of the population. The discovery of allelic forms of both the alpha and beta chains of the T cell antigen receptor genes may provide a unique opportunity to study heritable markers of T cell function in several human diseases.

scholarly journals Dominant expression of a distinctive V14+ T-cell antigen receptor alpha chain in mice.

1991 ◽  
Vol 88 (17) ◽  
pp. 7518-7522 ◽  
Author(s):  
H. Koseki ◽  
H. Asano ◽  
T. Inaba ◽  
N. Miyashita ◽  
K. Moriwaki ◽  
...  
Keyword(s):  
T Cell ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Receptor Alpha ◽  
Cell Antigen ◽  
Alpha Chain ◽  
Receptor Alpha Chain

scholarly journals Human T-cell antigen receptor (TCR) delta-chain locus and elements responsible for its deletion are within the TCR alpha-chain locus.

1988 ◽  
Vol 85 (24) ◽  
pp. 9694-9698 ◽  
Author(s):  
R. D. Hockett ◽  
J. P. de Villartay ◽  
K. Pollock ◽  
D. G. Poplack ◽  
D. I. Cohen ◽  
...  
Keyword(s):  
T Cell ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Alpha Chain ◽  
Chain Locus ◽  
Human T Cell

scholarly journals The structure of the T cell antigen receptor genes in normal and malignant T cells

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 327-336
Author(s):  
MD Minden ◽  
TW Mak
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Lineage ◽  
Antigen Receptor ◽  
Immunoglobulin Gene ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Alpha Chain ◽  
T Cell Malignancies

In this review the genomic structure and the RNA transcripts of the alpha and beta chain of the T cell antigen receptor have been discussed. Studies of the structure of TcR beta in hematologic malignancies have revealed rearrangement in almost all of the T cell malignancies and a small proportion of non-T cell malignancies. In addition, clonal involvement of T cells in diseases such as Hodgkin's disease, angioimmunoblastic lymphadenopathy, and chronic T cell lymphocytosis have been observed. The study of the structure of the TcR beta gene is thus a useful tool for identifying clonal expansions of cells and in conjunction with studies of the immunoglobulin gene structure, and cell surface markers a useful tool for identifying cell lineage. At the present time the evaluation of the structure of the alpha chain genes has not been as fruitful. However, chromosome translocations involving the TcR alpha chain genes have been recognized and, in one case, this rearrangement has been in association with a known oncogene. With the isolation of more probes to the alpha chain region it should be possible to test its utility in identifying clonal populations and cell lineage. The recent isolation of the gamma gene of the T cell will also permit such studies. Preliminary results of studies carried out with a probe to the gamma chain gene of the T cell have paralleled results obtained with the TcR beta probe (unpublished observation).

scholarly journals Rearrangements of T-cell antigen receptor delta chain gene in hematologic neoplasms

Blood ◽  
1989 ◽  
Vol 74 (8) ◽  
pp. 2707-2712 ◽  
Author(s):  
N Asou ◽  
T Hattori ◽  
M Matsuoka ◽  
F Kawano ◽  
K Takatsuki
Keyword(s):  
T Cell ◽  
B Cell ◽  
Gene Rearrangement ◽  
Antigen Receptor ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Delta Gene

Abstract Rearrangements of the T-cell antigen receptor (TCR) delta chain gene were studied in primary neoplastic cells from 137 patients with leukemia or lymphoma. TCR delta gene rearrangements or deletions were observed in all 50 T-cell neoplasms: 5 of 8 CD3- T-cell neoplasms showed rearrangements, whereas biallelic deletion of TCR delta gene was the most common pattern in CD3+ T-cell neoplasm (39 of 42 patients). Rearrangements of TCR delta gene were also detected in 23 of 40 immature B-cell leukemias, including 22 of 25 patients with rearrangements of TCR gamma gene, 2 of 17 mature B-cell neoplasms, and 3 of 30 myeloid leukemias. Thus, TCR delta gene rearrangement or deletion is always found in T-cell neoplasms and is frequently found in immature B-cell leukemias associated with TCR gamma gene rearrangement. Furthermore, TCR delta gene rearrangements associated with the germline configuration of the TCR beta, gamma, and immunoglobulin heavy chain genes were observed in two immature T-cell leukemias, suggesting that TCR delta gene rearrangements precede TCR gamma and beta gene rearrangements. These results indicate that an analysis of TCR delta gene rearrangement provides potential tools to establish the clonality of immature T-cell neoplasms and to identify the normal stages of lymphocyte differentiation.

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