scholarly journals Expression of T cell receptor genes in an antigen-specific hybridoma and radiation-induced variants.

1986 ◽  
Vol 164 (1) ◽  
pp. 113-130 ◽  
Author(s):  
N R Gascoigne ◽  
S Waters ◽  
J F Elliott ◽  
C Victor-Kobrin ◽  
C Goodnow ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Antigenic Determinants ◽  
Beta Chain ◽  
Stable Production ◽  
Radiation Induced ◽  
V Region ◽  
Beta Chain Genes ◽  
Antigen Reactivity

We have analyzed a series of mutants derived from a KLH-specific, I-E-restricted T hybridoma (FN1-18) which have lost antigen-reactivity while retaining both T cell receptor idiotypic determinants and the ability to respond to Con A. The variants have not gained any detectable alloreactivity, nor is there an obvious lesion in the mutants' beta chain DNA containing the utilized beta chain genes. This loss of antigen reactivity is due to a failure of stable production of the specific V beta-containing mRNA. Our results indicate that in FN1-18, the T cell receptor antigenic determinants are most likely carried by the alpha chain alone or by a complementation product of the V alpha FN1-18 with the V beta of BW5147. V beta FN1-18 represents a previously undescribed T cell receptor V region.

scholarly journals Human acute unclassified leukemia with a unique t(4;17) chromosomal translocation expresses T lymphoid and myeloid surface antigens after in vitro culture

Blood ◽  
1987 ◽  
Vol 69 (1) ◽  
pp. 271-277 ◽  
Author(s):  
A Ganser ◽  
G Heil ◽  
T Bohm ◽  
CR Bartram ◽  
A Raghavachar ◽  
...  
Keyword(s):  
T Cell ◽  
Suspension Culture ◽  
T Cell Receptor ◽  
Heavy Chain ◽  
Cell Receptor ◽  
Beta Chain ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes ◽  
Receptor Beta

Abstract Bilineage differentiation along both the T lymphoid and the myeloid lineage while in in vivo diffusion chamber (DC) and in vitro suspension culture was observed in a case of acute unclassified leukemia (null-AL) and t(4;17). Prior to culture, the blast cells were TdT and la positive but did not express any lineage-specific antigenic markers. Furthermore, the immunoglobulin heavy chain and T cell receptor beta- chain genes were in germline configuration. Cytogenetically, all metaphases had the unique translocation t(4;17) (q25;q23) prior to and after culture, supporting the leukemic origin of the cells. During both DC culture and suspension culture with and without tetradecanoyl- phorbol-acetate (TPA), a substantial increase in the absolute and relative number of cells expressing both myeloid and T lymphoid antigenic markers occurred. Double-fluorescence analysis demonstrated the expression of antigenic markers of both lineages on the same population of cells, and electron microscopy revealed the induction of myeloperoxidase after both DC and suspension culture. Immunoglobulin heavy chain and T cell receptor beta-chain genes remained in germline configuration after treatment with TPA, when analyzed with JH and CT beta probes, respectively. These findings indicate that this case represents a null-AL with dual-lineage capabilities, which has probably arisen from the malignant transformation of a bipotential stem cell of lymphoid and myeloid progeny.

scholarly journals Rearrangement of T-cell receptor beta-chain genes during T-cell development.

1985 ◽  
Vol 82 (9) ◽  
pp. 2925-2929 ◽  
Author(s):  
W. Born ◽  
J. Yague ◽  
E. Palmer ◽  
J. Kappler ◽  
P. Marrack
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Cell Development ◽  
Cell Receptor ◽  
Cell Development ◽  
Beta Chain ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes ◽  
Receptor Beta

scholarly journals Organization of human T-cell receptor beta-chain genes: clusters of V beta genes are present on chromosomes 7 and 9.

1993 ◽  
Vol 90 (6) ◽  
pp. 2433-2437 ◽  
Author(s):  
M. A. Robinson ◽  
M. P. Mitchell ◽  
S. Wei ◽  
C. E. Day ◽  
T. M. Zhao ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Beta Chain ◽  
Human T Cell ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes ◽  
Receptor Beta

scholarly journals T cell differentiation stages identified by molecular and immunologic analysis of the T cell receptor complex in childhood lymphoblastic leukemia

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 908-912 ◽  
Author(s):  
J Jr Mirro ◽  
G Kitchingman ◽  
FG Behm ◽  
SB Murphy ◽  
RM Goorha
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
T Cell Receptor ◽  
Lymphoblastic Leukemia ◽  
Cell Receptor ◽  
T Cell Differentiation ◽  
Receptor Complex ◽  
Beta Chain ◽  
Alpha Chain ◽  
Beta Chain Genes

Abstract T cell differentiation was investigated by determining the relationship of T cell receptor (Ti) gene rearrangement and transcription to the expression of surface and cytoplasmic T3 antigen using blast cells from five children with acute lymphoblastic leukemia of thymic origin. Patterns of monoclonal antibody (MoAb) reactivity indicated that these cases were representative of the three recognized stages (I, II, III) of human thymocyte development. The T3 antigen, which becomes linked to the Ti to form a functional T cell receptor complex on mature thymocytes, was expressed on the cell surface in two cases (stage III). However, in the remaining three cases that were surface T3 negative (stages I and II), large amounts of T3 were identified in the cytoplasm by immunoperoxidase staining and flow cytometry. Leukemic blasts from all five patients showed rearranged genes encoding the beta-chain portion of the Ti heterodimer. RNA transcripts of Ti beta-chain genes were also evident in lymphoblasts from all five cases, but transcripts coding for the alpha-chain portion of Ti were found only in cases that expressed T3 on the cell surface. Thus the absence of surface T3 (and presumably Ti) coincides with the absence of Ti alpha-chain RNA, suggesting that transcription of alpha-chain genes is a critical regulatory event in the surface expression of the Ti-T3 complex. Leukemic T cells that rearrange and express Ti beta-chain genes but lack Ti alpha-chain messenger RNA (mRNA) may represent a stage of differentiation analogous to pre-B cells, where heavy-chain immunoglobulin (Ig) genes are rearranged and expressed but light-chain Ig genes are not expressed.

Clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers with abnormal, non-flower-like, lymphocytes

2005 ◽  
Vol 75 (4) ◽  
pp. 280-287 ◽  
Author(s):  
Maria M. Sales ◽  
Camila N. A. Bezerra ◽  
Yumi Hiraki ◽  
Neusa B. Melo ◽  
Nancy A. Reboucas
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Beta Chain ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes ◽  
Receptor Beta

scholarly journals Dual T cell receptor beta chain expression on human T lymphocytes.

1995 ◽  
Vol 181 (4) ◽  
pp. 1391-1398 ◽  
Author(s):  
F Davodeau ◽  
M A Peyrat ◽  
F Romagné ◽  
A Necker ◽  
M M Hallet ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Allelic Exclusion ◽  
Beta Chain ◽  
Human T Lymphocytes ◽  
Repertoire Selection ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes

Allelic exclusion of lymphocyte antigen receptor chains has been hypothesized as a mechanism developed by the immune system to ensure efficient lymphocyte repertoire selection and tight control of lymphocyte specificity. It was effectively shown to be operative for both the immunoglobulin (Ig) and the T cell receptor (TCR) beta chain genes. Our present observations suggest that close to 1% of human T lymphocytes escape this allelic control, and express two surface TCR beta chains with distinct superantigenic reactivities. Since this high frequency of dual beta chain expressors did not result in any dramatic immune dysregulations, these results question the need for a mechanism ensuring clonal monospecificity through allelic exclusion.

Characterization of horse (Equus caballus) T-cell receptor beta chain genes

1994 ◽  
Vol 40 (2) ◽  
Author(s):  
MarkD. Schrenzel ◽  
JohannaL. Watson ◽  
DavidA. Ferrick
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Equus Caballus ◽  
Cell Receptor ◽  
Beta Chain ◽  
T Cell Receptor Beta ◽  
Beta Chain Genes ◽  
Receptor Beta
Sign in / Sign up

Export Citation Format

Share Document