scholarly journals The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.

1990 ◽  
Vol 171 (5) ◽  
pp. 1753-1771 ◽  
Author(s):  
J P Metlay ◽  
M D Witmer-Pack ◽  
R Agger ◽  
M T Crowley ◽  
D Lawless ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Peritoneal Macrophages ◽  
Mouse Spleen ◽  
White Pulp ◽  
Leukocyte Integrins ◽  
Binding Function ◽  
Murine Homologue ◽  
Lymph Node Cells ◽  
Beta 2

Hybridoma fusions with hamster hosts were undertaken to generate mAbs to mouse spleen dendritic cells. Two mAb were obtained and used to uncover the distinct integrins of these APC. One, 2E6, bound a determinant common to all members of the CD11/CD18 family, most likely the shared 90 kD CD18 beta chain. 2E6 immunoprecipitated the characteristic beta 2 integrin heterodimers from lymphocytes (p180, 90; CD11a) and macrophages (p170,90; CD11b), but from dendritic cells, a p150,90 (presumably CD11c) integrin was the predominant species. 2E6 inhibited the binding function of the CD11a and CD11b integrins on B cells and macrophages in appropriate assays, but 2E6 exerted little or no inhibition on the clustering of dendritic cells to T cells early in primary MLR, suggesting a CD11/CD18-independent mechanism for this binding. The second mAb, N418, precipitated a 150, 90 kD heterodimer that shared the 2E6 CD18 epitope. This N418 epitope may be the murine homologue of the previously characterized human CD11c molecule, but the epitope was only detected on dendritic cells. N418 did not react with peritoneal macrophages, anti-Ig-induced spleen B blasts, or bulk lymph node cells. When used to stain sections of spleen, N418 stained dendritic cells in the T-dependent areas, much like anti-class II mAbs that were also generated in these fusions. In addition, N418 revealed nests of dendritic cells that punctuated the rim of marginal zone macrophages between red and white pulp. This localization positioned most dendritic cells at regions where arterial vessels and T cells enter the white pulp. We conclude that the p150, 90 heterodimer is the major beta 2 integrin of spleen dendritic cells, and we speculate that it may function to localize these APC at sites that permit access to the recirculating pool of resting T cells.

scholarly journals Anatomy of germinal centers in mouse spleen, with special reference to "follicular dendritic cells"

1978 ◽  
Vol 77 (1) ◽  
pp. 148-164 ◽  
Author(s):  
LL Chen ◽  
JC Adams ◽  
RM Steinman
Keyword(s):  
Dendritic Cells ◽  
De Novo ◽  
Sheep Erythrocyte ◽  
Germinal Centers ◽  
Mouse Spleen ◽  
White Pulp ◽  
Follicular Dendritic Cells ◽  
Thorium Dioxide ◽  
Colloidal Carbon ◽  
Follicular Dendritic

Lymphocyte proliferation in germinal centers (GC's) is thought to be triggered by antigen retained extracellularly on the surface of special "dendritic" cells. The anatomy and function of these cells have not been studied directly or in detail. We therefore examined mouse spleen GC's developing in response to sheep erythrocyte stimulation. We found that distincitve "follicular dendritic cells" (FDC's) were present in both the GC and adjacent mantle region of secondary follicles. The large, irregularly shaped nucleus, containing little heterochromatin, allowed for the light microscope (LM) identification of FDC's. By EM, the cell was stellate in shape sending out long, thin sheets of cytoplasm which could fold and coil into complex arrays. The processes were coated extracellularly by an amorphous electron-dense material of varying thickness, as well as particulates including variable numbers of virions. The FDC cytoplasm lacked organelles of active secretory and endocytic cells, such as well-developed rough endoplasmic reticulum (RER) and lysosomes. These anatomical features readily distinguished FDC's from other cell types, even those that were extended in shape. To pursue these descriptive findings, we injected three electron-dense tracers i.v. and sacrificed the mice 1 h-10 days thereafter. Colloidal carbon, colloidal thorium dioxide (cThO2), and soluble horseradish peroxidase (HRP) were actively sequestered into the vacuolar system of macrophages but were interiorized only in trace amounts by FDC's. Therefore, FDC's are not macrophages by cytologic and functional criteria. FDC's did display a unique property. Both colloidal carbon and thorium dioxide, which are nonimmunogens, could be visualized extracellularly on the cell surface for several days. The meaning of this is unclear, but the association of colloid with FDC's appeared to slow the movement of particulates through the extracellular space into the GC proper. FDC's were not readily identified in splenic white pulp lacking GC's. They must develop de novo then, possibly from novel dendritic cells that we have identified in vitro (Steinman, R. M., and Z. A. Cohn. 1973. J. Exp. Med. 137:1142-1162).

scholarly journals Simultaneous Targeting of CD3 on T Cells and CD40 on B or Dendritic Cells Augments the Antitumor Reactivity of Tumor-Primed Lymph Node Cells

2005 ◽  
Vol 175 (3) ◽  
pp. 1424-1432 ◽  
Author(s):  
Qiao Li ◽  
Amelia C. Grover ◽  
Elizabeth J. Donald ◽  
Abbey Carr ◽  
Jiyun Yu ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Lymph Node Cells

scholarly journals Mouse spleen dendritic cells present soluble antigens to antigen-specific T cell hybridomas.

1983 ◽  
Vol 158 (5) ◽  
pp. 1745-1750 ◽  
Author(s):  
G H Sunshine ◽  
D P Gold ◽  
H H Wortis ◽  
P Marrack ◽  
J W Kappler
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Keyhole Limpet Hemocyanin ◽  
Mouse Spleen ◽  
Soluble Antigen

In the presence of the soluble polypeptide antigens ovalbumin and keyhole limpet hemocyanin, purified mouse spleen dendritic cells induce the secretion of IL-2 by antigen-specific T cell hybridomas. This response is H-2 restricted and can be specifically inhibited by monoclonal anti-I-A. These data indicate that dendritic cells can present soluble antigen to H-2-compatible T cells.

scholarly journals Migration patterns of dendritic cells in the mouse. Homing to T cell-dependent areas of spleen, and binding within marginal zone.

1988 ◽  
Vol 167 (2) ◽  
pp. 646-651 ◽  
Author(s):  
J M Austyn ◽  
J W Kupiec-Weglinski ◽  
D F Hankins ◽  
P J Morris
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Marginal Zone ◽  
Frozen Section ◽  
White Pulp ◽  
High Endothelial Venules ◽  
Migration Patterns ◽  
Interdigitating Cells ◽  
Mesenteric Lymph ◽  
Red Pulp

Using quantitative techniques we have shown elsewhere that dendritic cells (DC) migrate from blood into the spleen, under the control of T cells. Here we traced the localization of DC within the spleen and sought to explain the means by which they entered. DC were labeled with a fluorochrome, Hoescht 33342, and injected intravenously. Spleens were removed 3 or 24 h later and DC were visualized within particular areas that were defined by mAbs and FITC anti-Igs. At 3 h most DC were in the red pulp, whereas by 24 h the majority had homed to T-dependent areas of the white pulp and may have become interdigitating cells. Lymphoid DC, isolated from spleen and perhaps normally present in blood, may thus be a migratory stage distinct from the relatively fixed interdigitating cells. We also developed a frozen section assay to investigate the interaction of DC with various lymphoid elements. When DC were incubated on sections of spleen, at 37 degrees C but not at 4 degrees C they attached specifically within the marginal zone and did not bind to T areas; in contrast, macrophages attached only to red pulp and T cells did not bind specifically. However, DC did not bind to sections of mesenteric lymph node, whereas T cells localized in particular regions at 4 degrees C but not at 37 degrees C, probably the high endothelial venules. DC may thus express "homing receptors," similar to those of T cells, for certain endothelia. We propose that T cells can modify the vascular endothelium in certain areas to allow egress of DC from the bloodstream.

Generation of self-renewing immature dendritic cells from mouse spleen that can take up mycobacteria and present antigens to T cells

Apmis ◽  
2010 ◽  
Vol 118 (10) ◽  
pp. 729-738 ◽  
Author(s):  
RUBINA PAL ◽  
SARANDEEP MARWAHA ◽  
ILARIA PEPPONI ◽  
JAMIE F.S. MANN ◽  
MATTHEW J. PAUL ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Mouse Spleen ◽  
Immature Dendritic Cells

scholarly journals Restricted tissue distribution of Mlsa determinants. Stimulation of Mlsa-reactive T cells by B cells but not by dendritic cells or macrophages.

1989 ◽  
Vol 169 (1) ◽  
pp. 1-12 ◽  
Author(s):  
S R Webb ◽  
A Okamoto ◽  
Y Ron ◽  
J Sprent
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
B Cells ◽  
Tissue Distribution ◽  
Peritoneal Macrophages ◽  
Spleen Cells ◽  
Native Form ◽  
Form Part ◽  
Membrane Molecule ◽  
Stimulation Of

Evidence was sought on the tissue distribution of Mlsa determinants, a class of cell-associated non-H-2 alloantigens that is highly immunogenic for unprimed T cells. Whereas normal CD4+ T cells and an Mlsa-reactive T hybridoma gave strong responses to Mlsa-positive stimulator populations containing Ig+ B cells, anti-Mlsa responses to B-depleted stimulators were almost undetectable. The B-depleted stimulators tested included Thy-1- spleen cells from mu-suppressed mice (mice treated with anti-mu antibody from birth) and J11d- preparations of spleen dendritic cells (DC) and peritoneal macrophages (M phi) from normal mice. Each of these populations was strongly immunogenic for allo-H-2-reactive T cells. The failure to detect Mlsa determinants on Ig- APC, i.e., M phi and DC, suggests that Mlsa determinants are not typical H-2-associated peptides. The data are more compatible with a model in which Mlsa determinants represent (or form part of) an integral cell membrane molecule expressed largely, and perhaps exclusively, on B cells. T cells might recognize these molecules only in native form, "processed" Mlsa determinants being nonimmunogenic. Consistent with this possibility, no evidence was found that Mlsa-negative B cells could absorb Mlsa determinants from Mlsa-positive B cells in a chimeric environment.

Contribution of CD4+T Cells and Dendritic Cells to Female-Dominant Antigen-Induced T Helper Type 2 Cytokine Production by Bronchial Lymph Node Cells

2013 ◽  
Vol 161 (s2) ◽  
pp. 58-65 ◽  
Author(s):  
Kaori Okuyama ◽  
Masatoshi Suenaga ◽  
Shyunya Furuki ◽  
Tasuku Kawano ◽  
Yuichi Ohkawara ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Cd4 T Cells ◽  
Cytokine Production ◽  
T Helper ◽  
Lymph Node Cells ◽  
Bronchial Lymph Node ◽  
Helper Type

ATaenia crassicepsfactor induces apoptosis of spleen CD4+T cells and TFG-β andFoxp3gene expression in mice

2015 ◽  
Vol 90 (2) ◽  
pp. 223-231 ◽  
Author(s):  
N. Zepeda ◽  
R. Tirado ◽  
N. Copitin ◽  
S. Solano ◽  
A.M. Fernández ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Transforming Growth Factor ◽  
Ex Vivo ◽  
Mouse Spleen ◽  
White Pulp ◽  
Spleen Cells ◽  
Parasite Relationship ◽  
Potential Applications

AbstractThis study was undertaken to determine whether a parasite substance produces structural pathology in the mouse spleen. A low-molecular-weightTaenia crassicepsmetacestode factor (MF) isolated from the peritoneal fluid of female mice infected withT. crassicepsmetacestodes induced pathological and immunological changes in mouse spleen cellsin vivo.Electron microscopy and confocal microscopy revealed severe changes in the spleen histoarchitecture ofT. crassiceps-infected and MF-treated mice. Apoptotic degenerated spleen cells were observed in the white and red pulps and were more conspicuous in the white pulp of the spleen from theT. crassiceps-infected mice than in that of the MF-treated mice. Flow cytometry analysis revealed that the numbers of spleen CD4+T cells were significantly lower in both experimental groups than in control mice. Theex vivoexpression of transforming growth factor (TGF)-β and factor Foxp3 were significantly higher in splenocytes of the experimental mice than the basal expression observed in the control cells. These findings may have potential applications for a better understanding of the host–parasite relationship in human neurocysticercosis.

scholarly journals Identification of a novel cell type in peripheral lymphoid organs of mice. IV. Identification and distribution in mouse spleen.

1975 ◽  
Vol 141 (4) ◽  
pp. 804-820 ◽  
Author(s):  
R M Steinman ◽  
J C Adams ◽  
Z A Cohn
Keyword(s):  
Dendritic Cells ◽  
Cell Types ◽  
Lymphoid Organs ◽  
Mouse Spleen ◽  
White Pulp ◽  
Cell Type ◽  
Thorium Dioxide ◽  
Cell Shapes ◽  
A Minor

White pulp nodules of mouse spleen contain a minor population of cells with morphologic features that are identical to those of dendritic cells, a cell type recently described in vitro. They have characteristic large, irregularly shaped nuclei with distinctive chromatin patterns and small nucleoli. The cytoplasm is extended in processes that contain relatively few organelles. These presumptive dendritic cells can be distinguished from other cell types that are known to exist in spleen including those that have irregular or branching cell shapes. In particular, dendritic cells do not contain the large number of lysosomes seen in phagocytes, and do not actively interiorize intravenously administered colloidal thorium dioxide particles. They also lack the well developed secretory apparatus (rough endoplasmic reticulum and Golgi zone) and microfilament bundles that are noted in connective tissue cells. These morphologic observations, combined with previous in vitro work, substantiate the existence of a novel class of cells in mouse lymphoid organs.

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