scholarly journals Vaccination with Heat-killed Leishmania Antigen or Recombinant Leishmanial Protein and CpG Oligodeoxynucleotides Induces Long-Term Memory CD4+and CD8+T Cell Responses and Protection Against Leishmania major Infection

2002 ◽  
Vol 195 (12) ◽  
pp. 1565-1573 ◽  
Author(s):  
Elizabeth G. Rhee ◽  
Susana Mendez ◽  
Javeed A. Shah ◽  
Chang-you Wu ◽  
Joanna R. Kirman ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Vaccine Adjuvant ◽  
Dependent Manner ◽  
Cpg Odn ◽  
Major Infection ◽  
Cpg Oligodeoxynucleotides ◽  
Immune Correlates

CpG oligodeoxynucleotides (ODN) have potent effects on innate and adaptive cellular immune responses. In this report, the ability of CpG ODN to confer long-term immunity and protection when used as a vaccine adjuvant with a clinical grade of leishmanial antigen, autoclaved Leishmania major (ALM), or a recombinant leishmanial protein was studied. In two different mouse models of L. major infection, vaccination with ALM plus CpG ODN was able to control infection and markedly reduce lesion development in susceptible BALB/c and resistant C57BL/6 (B6) mice, respectively, up to 12 wk after immunization. Moreover, B6 mice immunized with ALM plus CpG ODNs were still protected against infectious challenge even 6 mo after vaccination. In terms of immune correlates of protection, ALM plus CpG ODN-vaccinated mice displayed L. major–specific T helper cell 1 and CD8+ responses. In addition, complete protection was markedly abrogated in mice depleted of CD8+ T cells at the time of vaccination. Similarly, mice vaccinated with a recombinant leishmanial protein plus CpG ODN also had long-term protection that was dependent on CD8+ T cells in vivo. Together, these data demonstrate that CpG ODN, when used as a vaccine adjuvant with either a recombinant protein or heat-killed leishmanial antigen, can induce long-term protection against an intracellular infection in a CD8-dependent manner.

scholarly journals Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection

2008 ◽  
Vol 80 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Wânia F. Pereira ◽  
Landi V.C. Guillermo ◽  
Flávia L. Ribeiro-Gomes ◽  
Marcela F. Lopes
Keyword(s):  
T Cells ◽  
T Cell ◽  
Lymph Nodes ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Caspase 8 ◽  
Ifn Gamma ◽  
Major Infection ◽  
Draining Lymph Nodes

Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following Tcellactivation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-FasLigand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile- Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-gamma expression in both CD4 and CD8T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediated type-1 responses during L. major infection.

scholarly journals Establishment of resistance to Leishmania major infection in susceptible BALB/c mice requires parasite-specific CD8+ T cells

1991 ◽  
Vol 3 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Ingrid Müller ◽  
Thierry Pedrazzini ◽  
Pascale Kropf ◽  
Jacques Louis ◽  
Geneviève Milon
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Major Infection

scholarly journals Repertoire, diversity, and differentiation of specific CD8 T cells are associated with immune protection against human cytomegalovirus disease

2005 ◽  
Vol 201 (12) ◽  
pp. 1999-2010 ◽  
Author(s):  
Karim Sacre ◽  
Guislaine Carcelain ◽  
Nathalie Cassoux ◽  
Anne-Marie Fillet ◽  
Dominique Costagliola ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
T Cell Response ◽  
Hiv Positive ◽  
Immune Protection ◽  
Immune Correlates ◽  
Hcmv Disease ◽  
Repertoire Diversity ◽  
Human Cmv

To determine the correlates of immune recovery from active human CMV (HCMV) disease, we compared the antigenic repertoire, diversity, magnitude, and differentiation of HCMV-specific CD8+ T cells in HIV-HCMV coinfected subjects with no, cured, or active HCMV disease and in healthy HIV-negative HCMV-positive controls. ELISPOT–IFN-γ assays using peptide pools spanning the pp65 and immediate early 1 (IE1) HCMV proteins showed that HCMV-specific CD8+ T cells had a significantly broader antigenic repertoire and greater diversity in HIV-positive patients controlling HCMV replication than in those with active HCMV disease, but the magnitude of the CD8 T cell response did not differ between the different groups. HCMV-specific T cells mainly were focused against IE1 during the short-term recovery from retinitis, and switched toward pp65 during long-term recovery. HCMV-specific T cells displaying an “early” (CD8+CD27+CD28+) and “intermediate” (CD8+CD27−CD28+) differentiation phenotype were increased significantly during long-term recovery compared with other HIV-positive patients and were nearly undetectable during active HCMV disease. HCMV-specific T cells with a “late” (CD8+CD27−28−) differentiation phenotype predominated in all cases. Therefore, restoration of immune protection against HCMV after active HCMV disease in immunodeficient individuals is associated with enlarged repertoire and diversity, and with early differentiation of virus-specific CD8+ T cells, thus defining immune correlates of protection against diseases caused by persistent viruses.

scholarly journals Engagement of NKG2D on Bystander Memory CD8 T Cells Promotes Increased Immunopathology following Leishmania major Infection

2014 ◽  
Vol 10 (2) ◽  
pp. e1003970 ◽  
Author(s):  
Erika J. Crosby ◽  
Michael H. Goldschmidt ◽  
E. John Wherry ◽  
Phillip Scott
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Memory Cd8 T Cells ◽  
Major Infection
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