scholarly journals Gamma interferon response in secondary Leishmania major infection: role of CD8+ T cells.

1993 ◽  
Vol 61 (9) ◽  
pp. 3730-3738 ◽  
Author(s):  
I Müller ◽  
P Kropf ◽  
R J Etges ◽  
J A Louis
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Gamma Interferon ◽  
Interferon Response ◽  
Major Infection

scholarly journals Inhibition of caspase-8 activity reduces IFN-gamma expression by T cells from Leishmania major infection

2008 ◽  
Vol 80 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Wânia F. Pereira ◽  
Landi V.C. Guillermo ◽  
Flávia L. Ribeiro-Gomes ◽  
Marcela F. Lopes
Keyword(s):  
T Cells ◽  
T Cell ◽  
Lymph Nodes ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Caspase 8 ◽  
Ifn Gamma ◽  
Major Infection ◽  
Draining Lymph Nodes

Following infection with Leishmania major, T cell activation and apoptosis can be detected in draining lymph nodes of C57BL/6-infected mice. We investigated the mechanisms involved in apoptosis and cytokine expression following Tcellactivation. After two weeks of infection, apoptotic T cells were not detected in draining lymph nodes but activation with anti-CD3 induced apoptosis in both CD4 and CD8 T cells. Treatment with anti-FasLigand, caspase-8 or caspase- 9 inhibitors did not block activation-induced T-cell death. We also investigated whether the blockade of caspase-8 activity would affect the expression of type-1 or type-2 cytokines. At early stages of infection, both CD4 and CD8 T cells expressed IFN-gamma upon activation. Treatment with the caspase-8 inhibitor zIETD-fmk (benzyl-oxycarbonyl-Ile- Glu(OMe)-Thr-Asp(OMe)-fluoromethyl ketone) reduced the proportion of CD8 T cells and IFN-gamma expression in both CD4 and CD8T cells. We conclude that a non apoptotic role of caspase-8 activity may be required for T cell-mediated type-1 responses during L. major infection.

scholarly journals Protection against Mycobacterium tuberculosisInfection by CD8+ T Cells Requires the Production of Gamma Interferon

1998 ◽  
Vol 66 (2) ◽  
pp. 830-834 ◽  
Author(s):  
Ricardo E. Tascon ◽  
Evangelos Stavropoulos ◽  
Katalin V. Lukacs ◽  
M. Joseph Colston
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Protective Immunity ◽  
Gamma Interferon ◽  
Cell Populations ◽  
Athymic Mice ◽  
Histocompatibility Complex ◽  
Cd8 Cell ◽  
Ifn Γ

ABSTRACT The role of CD8 T cells in controlling Mycobacterium tuberculosis infections in mice was confirmed by comparing the levels of growth of the organism in control, major histocompatibility complex class II knockout, and athymic mice and by transferring T-cell populations into athymic mice. By using donor mice which were incapable of making gamma interferon (IFN-γ), it was shown that IFN-γ production was essential for CD8 cell mediation of protective immunity against M. tuberculosis.

scholarly journals Establishment of resistance to Leishmania major infection in susceptible BALB/c mice requires parasite-specific CD8+ T cells

1991 ◽  
Vol 3 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Ingrid Müller ◽  
Thierry Pedrazzini ◽  
Pascale Kropf ◽  
Jacques Louis ◽  
Geneviève Milon
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Major Infection

scholarly journals Engagement of NKG2D on Bystander Memory CD8 T Cells Promotes Increased Immunopathology following Leishmania major Infection

2014 ◽  
Vol 10 (2) ◽  
pp. e1003970 ◽  
Author(s):  
Erika J. Crosby ◽  
Michael H. Goldschmidt ◽  
E. John Wherry ◽  
Phillip Scott
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Memory Cd8 T Cells ◽  
Major Infection

scholarly journals Vaccination with Heat-killed Leishmania Antigen or Recombinant Leishmanial Protein and CpG Oligodeoxynucleotides Induces Long-Term Memory CD4+and CD8+T Cell Responses and Protection Against Leishmania major Infection

2002 ◽  
Vol 195 (12) ◽  
pp. 1565-1573 ◽  
Author(s):  
Elizabeth G. Rhee ◽  
Susana Mendez ◽  
Javeed A. Shah ◽  
Chang-you Wu ◽  
Joanna R. Kirman ◽  
...  
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Leishmania Major ◽  
Vaccine Adjuvant ◽  
Dependent Manner ◽  
Cpg Odn ◽  
Major Infection ◽  
Cpg Oligodeoxynucleotides ◽  
Immune Correlates

CpG oligodeoxynucleotides (ODN) have potent effects on innate and adaptive cellular immune responses. In this report, the ability of CpG ODN to confer long-term immunity and protection when used as a vaccine adjuvant with a clinical grade of leishmanial antigen, autoclaved Leishmania major (ALM), or a recombinant leishmanial protein was studied. In two different mouse models of L. major infection, vaccination with ALM plus CpG ODN was able to control infection and markedly reduce lesion development in susceptible BALB/c and resistant C57BL/6 (B6) mice, respectively, up to 12 wk after immunization. Moreover, B6 mice immunized with ALM plus CpG ODNs were still protected against infectious challenge even 6 mo after vaccination. In terms of immune correlates of protection, ALM plus CpG ODN-vaccinated mice displayed L. major–specific T helper cell 1 and CD8+ responses. In addition, complete protection was markedly abrogated in mice depleted of CD8+ T cells at the time of vaccination. Similarly, mice vaccinated with a recombinant leishmanial protein plus CpG ODN also had long-term protection that was dependent on CD8+ T cells in vivo. Together, these data demonstrate that CpG ODN, when used as a vaccine adjuvant with either a recombinant protein or heat-killed leishmanial antigen, can induce long-term protection against an intracellular infection in a CD8-dependent manner.

scholarly journals CD8 T Cells in Old Mice Contribute to the Innate Immune Response to Mycobacterium tuberculosis via Interleukin-12p70-Dependent and Antigen-Independent Production of Gamma Interferon

2009 ◽  
Vol 77 (8) ◽  
pp. 3355-3363 ◽  
Author(s):  
Bridget Vesosky ◽  
Erin K. Rottinghaus ◽  
Craig Davis ◽  
Joanne Turner
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Cd8 T Cells ◽  
The Elderly ◽  
Gamma Interferon ◽  
Ifn Γ ◽  
Aerosol Infection ◽  
Old Mice

ABSTRACT Elderly individuals have increased morbidity and mortality associated with infectious diseases due in part to the progressive age-associated decline in immune function. Despite this, the old mouse model of Mycobacterium tuberculosis infection has revealed a CD8- and gamma interferon (IFN-γ)-dependent early resistance to infection. In this study, we investigated the mechanism by which CD8 T cells from old mice contributed to the early immune response to M. tuberculosis. Following a low-dose aerosol infection with M. tuberculosis, CD8 T cells were identified as being a dominant source of IFN-γ expression in the lungs of old mice early after infection, before the typical onset of antigen-specific immunity. In addition, M. tuberculosis-induced IFN-γ production by CD8 T cells isolated from naïve old mice was major histocompatibility complex class I independent but was dependent on interleukin-12p70, confirming an innate role of CD8 T cells during M. tuberculosis infection. Moreover, the ability of CD8 T cells from old mice to produce increased innate IFN-γ levels in response to M. tuberculosis infection was defined as a unique function of CD8 T cells from old mice and not the aged lung environment. Finally, we have identified increased expression of SET as being one possible mechanism by which CD8 T cells from old mice produce enhanced levels of IFN-γ. Additional characterizations of the signaling events that lead to enhanced innate IFN-γ production by CD8 T cells in old mice may lead to novel strategies to further enhance or perpetuate beneficial immune responses in the elderly.

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