scholarly journals In Vitro Model for the Study of the Dissociation of Increasing Antigenemia and Decreasing DNAemia and Viremia during Treatment of Human Cytomegalovirus Infection with Ganciclovir in Transplant Recipients

2003 ◽  
Vol 188 (11) ◽  
pp. 1639-1647 ◽  
Author(s):  
Giuseppe Gerna ◽  
Antonella Sarasini ◽  
Daniele Lilleri ◽  
Elena Percivalle ◽  
Maria Torsellini ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
In Vitro Model ◽  
Transplant Recipients ◽  
Human Cytomegalovirus Infection ◽  
Vitro Model

scholarly journals Human Cytomegalovirus Infection Enhances NK Cell Activity In Vitro

2016 ◽  
Vol 2 (7) ◽  
pp. e89 ◽  
Author(s):  
Astrid Tschan-Plessl ◽  
Martin Stern ◽  
Laurent Schmied ◽  
Christelle Retière ◽  
Hans H. Hirsch ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
Human Cytomegalovirus Infection

scholarly journals Innate Nuclear Sensor IFI16 Translocates into the Cytoplasm during the Early Stage of In Vitro Human Cytomegalovirus Infection and Is Entrapped in the Egressing Virions during the Late Stage

2014 ◽  
Vol 88 (12) ◽  
pp. 6970-6982 ◽  
Author(s):  
V. Dell'Oste ◽  
D. Gatti ◽  
F. Gugliesi ◽  
M. De Andrea ◽  
M. Bawadekar ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Late Stage ◽  
Cytomegalovirus Infection ◽  
Early Stage ◽  
Human Cytomegalovirus Infection

Human cytomegalovirus infection in a retinoblastoma cell line in vitro

1998 ◽  
Vol 236 (12) ◽  
pp. 945-949
Author(s):  
Yuji Kumano ◽  
Keith A. Laycock ◽  
Kelly K. Hook ◽  
J. S. Pepose ◽  
Patrick M. Stuart
Keyword(s):  
Cell Line ◽  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Retinoblastoma Cell ◽  
Retinoblastoma Cell Line ◽  
Human Cytomegalovirus Infection

scholarly journals Common Features of γδ T Cells and CD8+αβ T Cells Responding to Human Cytomegalovirus Infection in Kidney Transplant Recipients

2009 ◽  
Vol 200 (9) ◽  
pp. 1415-1424 ◽  
Author(s):  
Lionel Couzi ◽  
Vincent Pitard ◽  
Sonia Netzer ◽  
Isabelle Garrigue ◽  
Marie‐Edith Lafon ◽  
...  
Keyword(s):  
T Cells ◽  
Kidney Transplant ◽  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Γδ T Cells ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Common Features ◽  
Human Cytomegalovirus Infection ◽  
Αβ T Cells

scholarly journals The Major Open Reading Frame of the β2.7 Transcript of Human Cytomegalovirus: In Vitro Expression of a Protein Posttranscriptionally Regulated by the 5′ Region

1998 ◽  
Vol 72 (10) ◽  
pp. 8425-8429 ◽  
Author(s):  
Giovanna Bergamini ◽  
Marko Reschke ◽  
Maria Concetta Battista ◽  
Maria Cristina Boccuni ◽  
Fabio Campanini ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Intracellular Localization ◽  
Protein Product ◽  
Transient Transfection ◽  
Open Reading Frame ◽  
Major Open Reading Frame ◽  
Reading Frame ◽  
Human Cytomegalovirus Infection

ABSTRACT β2.7 is the major early transcript produced during human cytomegalovirus infection. This abundantly expressed RNA is polysome associated, but no protein product has ever been detected. In this study, a stable peptide of 24 kDa was produced in vitro from the major open reading frame (ORF), TRL4. Following transient transfection, the intracellular localization was nucleolar and the expression was posttranscriptionally inhibited by the 5′ sequence of the transcript, which harbors two short upstream ORFs.

Human Cytomegalovirus Infection of Primitive Hematopoietic Progenitor Cells: A Model for Latency.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1703-1703
Author(s):  
Felicia D. Goodrum ◽  
Craig T. Jordan ◽  
Scott S. Terhune ◽  
Kevin P. High ◽  
Thomas Shenk
Keyword(s):  
Bone Marrow ◽  
Viral Replication ◽  
Progenitor Cells ◽  
Human Cytomegalovirus ◽  
In Vitro Model ◽  
Latent Infection ◽  
Cmv Infection ◽  
Aids Patients ◽  
Vitro Model

Abstract Human cytomegalovirus (CMV) is beta-herpesvirus that infects the majority of the population worldwide and establishes a lifelong relationship with its host by way of a latent infection. Although, CMV is of little consequence to healthy individuals, reactivation of CMV from latency can cause life-threatening disease in immunocompromised individuals such as bone marrow transplant recipients and AIDS patients. CMV establishes a latent infection in cells of the hematopoietic system. The focus of our work is to elucidate the mechanisms governing a latent infection and reactivation from latency. It is likely that both viral and cellular mechanisms govern the latent program. We have developed a novel in vitro model for CMV latency using primary human hematopoietic progenitors. Using this model to compare CMV infection in CD34+ hematopoietic subpopulations, the outcome of CMV infection was found to depend on the nature of the subpopulation of cells infected. Only primitive CD34+/CD38− progenitor cells harboured an infection with the hallmarks of latency. This subpopulation transiently expressed a unique subset of CMV genes in the absence of viral replication. Importantly, viral replication could be reactivated from this subpopulation. Other primitive (CD34+/c-kit+) and more mature (CD34+/CD38+ and CD34+/c-kit−) subpopulations resulted in an apparently abortive infection. Strikingly, a CD34+ subpopulation expressing a stem cell phenotype supported a productive infection. These results indicate that cellular determinants contribute to the outcome of HCMV infection in hematopoietic cells. Further, we have also demonstrated that viral determinants play a role in establishing a latent infection using our in vitro model. Comparing the clinical Toledo and FIX strains and the laboratory-adapted AD169 strain of CMV, only the clinical strains were able to establish an infection consistent with latency. AD169, by contrast, replicated productively. Genetic sequences unique to clinical strains have been identified and may contribute to its ability to establish a latent infection in vitro. These studies indicate that both viral and cellular determinants contribute to the establishment of CMV latency. Furthermore, these determinants may serve as targets for CMV disease prophylaxis following bone marrow transplantation or in AIDS patients.

Human Cytomegalovirus Infection in Transplant Recipients: Role of TNF-α for Reactivation and Replication of Human Cytomegalovirus

1997 ◽  
pp. 29-41 ◽  
Author(s):  
S. Pr�sch ◽  
H.-D. Volk ◽  
P. Reinke ◽  
K. Pioch ◽  
W.-D. D�cke ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Tnf Alpha ◽  
Transplant Recipients ◽  
Human Cytomegalovirus Infection

scholarly journals B-cell-activating factor code and human cytomegalovirus infection in renal transplant recipients

2014 ◽  
Vol 58 (8) ◽  
pp. 439-448 ◽  
Author(s):  
Haiyan Xu ◽  
Panpan Dong ◽  
Xiaozhou He ◽  
Xuyi Ma ◽  
Dong Xue ◽  
...  
Keyword(s):  
Renal Transplant ◽  
B Cell ◽  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
B Cell Activating Factor ◽  
Human Cytomegalovirus Infection ◽  
Factor Code
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