Usefulness of Pulsed-Field Gel Electrophoresis in Assessing Nosocomial Transmission of Pertussis

1999 ◽  
Vol 20 (11) ◽  
pp. 758-760 ◽  
Author(s):  
Michèle Nouvellon ◽  
Jean-François Gehanno ◽  
Martine Pestel-Caron ◽  
Christian Weber ◽  
Jean-François Lemeland ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Gel Electrophoresis ◽  
Healthcare Workers ◽  
Whooping Cough ◽  
Close Contact ◽  
Pulsed Field Gel Electrophoresis ◽  
Nosocomial Transmission ◽  
Respiratory Protection ◽  
Pulsed Field ◽  
Protection Devices

AbstractDuring a 2-week period, three infants with a cough lasting at least 8 days with whoops, were admitted to the pediatric unit; Bordetella pertussis was isolated from nasopharyngeal aspirates collected from the three infants. Approximately 1 week later, a nurse working on the same unit developed influenza-like symptoms followed by whooping cough; B pertussis was isolated. Isolates from the nurse and from one of the infants were shown to be indistinguishable by pulsed-field gel electrophoresis. These data demonstrate that B pertussis transmission to healthcare workers is possible and emphasize the need to use respiratory protection devices (Droplet Precautions) for healthcare workers having close contact with infected children.

scholarly journals Bordetella pertussis Strains Circulating in Europe in 1999 to 2004 as Determined by Pulsed-Field Gel Electrophoresis

2007 ◽  
Vol 45 (10) ◽  
pp. 3257-3262 ◽  
Author(s):  
H. Hallander ◽  
A. Advani ◽  
M. Riffelmann ◽  
C. H. W. von Konig ◽  
V. Caro ◽  
...  
Keyword(s):  
Bordetella Pertussis ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Application of pulsed field gel electrophoresis to the 1993 epidemic of whooping cough in the UK

1995 ◽  
Vol 115 (1) ◽  
pp. 101-113 ◽  
Author(s):  
S. N. Syedabubakar ◽  
R. C. Matthews ◽  
N. W. Preston ◽  
D. Owen ◽  
V. Hillier
Keyword(s):  
Gel Electrophoresis ◽  
Whooping Cough ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Reference Laboratory ◽  
Age Group ◽  
Pulsed Field ◽  
Significant Difference ◽  
Dna Types

SummaryThe purpose of this study was to DNA fingerprint the majority (64 %) of isolates received at the Pertussis Reference Laboratory during the 1993 whooping cough epidemic by pulsed field gel electrophoresis of Xba I - generated restriction digests. Two DNA restriction patterns, types 1 and 3, predominated (40% and 23%. respectively, of 180 isolates) but type 2, identified in a previous study was notably absent. Twenty-one new DNA types occurred (24% of isolates), some being atypical as bands 155–230 kb were no longer conserved, but there was no statistically significant difference in their incidence in the upswing (June-September) compared to the downswing (October-December) phase of the epidemic. There was a relatively high proportion of new types, compared to type 1. at the peak (September). About 50% of isolates received were from the North Western Region, where 44% of isolates were DNA type 1. Whereas only 1 out of 10 isolates from Scotland were of this type, suggesting some geographic variation. Statistically significant findings included a higher proportion of isolates from female patients (P < 0·01), most marked in the 12–24 months age group (P < 0·05); a higher proportion of infants under 12 months requiring hospital admission compared to older children (P < 0·05); and a greater number of isolates from unvaccinated children (P < 0·01). Analysis of serotype according to four age groups (under 3 months, 3–12 months, 12–24 months and above 2 years) showed statistically significant differences (P < 0·05) with a noticeably lower proportion (38%) of serotype 1,3 in 3–12 months age group and higher prevalence (74%) of serotype 1,3 in the 12–24 months age group. There was no correlation between DNA type and serotype.

scholarly journals Pulsed-Field Gel Electrophoresis, Pertactin, Pertussis Toxin S1 Subunit Polymorphisms, and Surfaceome Analysis of Vaccine and Clinical Bordetella pertussis Strains

2007 ◽  
Vol 14 (11) ◽  
pp. 1490-1498 ◽  
Author(s):  
Daniela Bottero ◽  
María Emilia Gaillard ◽  
Matías Fingermann ◽  
Gabriela Weltman ◽  
Julieta Fernández ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Bordetella Pertussis ◽  
Gel Electrophoresis ◽  
Elimination Rate ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Mass Spectrometry Analysis ◽  
Group Vi ◽  
Pulsed Field ◽  
Vaccine Type

ABSTRACT To add new insight to our previous work on the molecular epidemiology of Bordetella pertussis in Argentina, the prn and ptxS1 gene sequences and pulsed-field gel electrophoresis (PFGE) profiles of 57 clinical isolates obtained during two periods, 1969 to 1989 and 1997 to 2006, were analyzed. Non-vaccine-type ptxS1A was detected in isolates obtained since 1969. From 1989 on, a shift of predominance from the vaccine prn1 type to the nonvaccine prn2 type was observed. This was also reflected in a transition of PFGE group IV to group VI. These results show that nonvaccine B. pertussis strains are currently circulating. To analyze whether the observed genomic divergences between vaccine strains and clinical isolates have functional implications, protection assays using the intranasal mouse challenge model were performed. For such experiments, the clinical isolate B. pertussis 106 was selected as representative of circulating bacteria, since it came from the major group of the PFGE dendrogram (PFGE group VI). Groups of mice were immunized either with diphtheria-tetanus-whole-cell pertussis vaccine (ptxS1B prn1) or a vaccine prepared by us containing B. pertussis 106. Immunized mice were then challenged with a B. pertussis vaccine strain (Tohama, harboring ptxS1B and prn1) or the clinical isolate B. pertussis 106 (ptxS1A prn2). An adequate bacterial-elimination rate was observed only when mice were immunized and challenged with the same kind of strain. For further characterization, comparative proteomic profiling of enriched membrane proteins was done using three vaccine strains and the selected B. pertussis 106 clinical isolate. By matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis, a total of 54 proteins were identified. This methodology allowed us to detect differing proteins among the four strains studied and, in particular, to distinguish the three vaccine strains from each other, as well as the vaccine strains from the clinical isolate. The differing proteins observed have cellular roles associated with amino acid and carbohydrate transport and metabolism. Some of them have been proposed as novel vaccine candidate proteins for other pathogens. Overall, the global strategy described here is presented as a good tool for the development of next-generation acellular vaccines.

Molecular Typing ofAgrobacteriumSpecies Isolates From Catheter-Related Bloodstream Infections

2004 ◽  
Vol 25 (10) ◽  
pp. 885-887 ◽  
Author(s):  
Giovanni M. Giammanco ◽  
Sarina Pignato ◽  
Carmelita Santangelo ◽  
Patrick A. D. Grimont ◽  
Francine Grimont ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Bloodstream Infections ◽  
Pulsed Field Gel Electrophoresis ◽  
Hospitalized Patients ◽  
Nosocomial Transmission ◽  
Pulsed Field ◽  
Intravenous Catheters ◽  
Rdna Analysis

AbstractAgrobacteriumisolates from intravenous catheters of three hospitalized patients were initially identified asA. tumefaciens,but inability to produce 3-ketolactose revealed that two of them wereA. vitis.However, rDNA analysis correlated all of the isolates toA. tumefaciens.Pulsed-field gel electrophoresis analysis ascertained the nosocomial transmission of the infection.

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