Detection of 4 imperiled western North American freshwater mussel species from environmental DNA with multiplex qPCR assays

AbstractWe developed multiplexed, species-specific, quantitative PCR assays for the detection of four freshwater mussel species native to western North America, Gonidea angulata, Margaritifera falcata, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA (eDNA). These species have experienced dramatic declines over the last century and are currently threatened in many portions of their ranges. Therefore, improved tools for detecting and monitoring these species are needed. Species-specificity and sensitivity of assays were empirically tested in the lab, and multiplex assays were also validated with field collected eDNA samples. All assays were species-specific, sensitive, and effective for detection from eDNA samples collected from streams and rivers. These assays will aid in the detection, monitoring, management, and conservation of these vulnerable species.

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Revisiting the North American freshwater mussel genus Quadrula sensu lato (Bivalvia Unionidae): Phylogeny, taxonomy and species delineation

Zoologica Scripta ◽

10.1111/zsc.12344 ◽

2019 ◽

Vol 48 (3) ◽

pp. 313-336 ◽

Cited By ~ 3

Author(s):

Manuel Lopes‐Lima ◽

Lyubov Burlakova ◽

Alexander Karatayev ◽

André Gomes‐dos‐Santos ◽

Alexandra Zieritz ◽

...

Keyword(s):

North American ◽

Freshwater Mussel ◽

Species Delineation ◽

The North ◽

North American Freshwater

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Complete mtDNA Sequence of the North American Freshwater Mussel, Lampsilis ornata (Unionidae): An Examination of the Evolution and Phylogenetic Utility of Mitochondrial Genome Organization in Bivalvia (Mollusca)

Molecular Biology and Evolution ◽

10.1093/molbev/msg218 ◽

2003 ◽

Vol 20 (11) ◽

pp. 1854-1866 ◽

Cited By ~ 96

Author(s):

J. M. Serb

Keyword(s):

Mitochondrial Genome ◽

North American ◽

Genome Organization ◽

Freshwater Mussel ◽

The North ◽

North American Freshwater ◽

Mtdna Sequence ◽

Complete Mtdna

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Infestation of the North American freshwater mussel Elliptio complanata (Head Lake, Canada) by the euendolithic cyanobacterium Plectonema terebrans Bornet et Flahault

Algological Studies ◽

10.1127/1864-1318/2008/0128-0065 ◽

2008 ◽

Vol 128 ◽

pp. 65-77 ◽

Cited By ~ 7

Author(s):

Aline Tribollet ◽

Geoff Veinott ◽

Stjepko Golubic ◽

Ray Dart

Keyword(s):

North American ◽

Freshwater Mussel ◽

Elliptio Complanata ◽

The North ◽

North American Freshwater

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qPCR assays for the detection of two western North American freshwater mussel species, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA

10.1101/372219 ◽

2018 ◽

Author(s):

Torrey W. Rodgers ◽

Karen E. Mock

Keyword(s):

North America ◽

Threatened Species ◽

Freshwater Mussel ◽

Environmental Dna ◽

Western North America ◽

Mussel Species ◽

Specificity And Sensitivity ◽

Pcr Assays ◽

Species Specific ◽

Management And Conservation

AbstractWe developed species-specific quantitative PCR assays for the detection of two freshwater mussel species native to the western North America, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA. These species have experienced dramatic declines over the last century, and are currently threatened in many portions of their range. Improved tools for detecting and monitoring these species are needed. Species-specificity and sensitivity of the assays was empirically tested in the lab, and both assays were also validated with field collected eDNA samples. We found that the assays we designed are species-specific, sensitive, and are effective for detecting Anodonta nuttalliana and Anodonta oregonensis from environmental DNA samples collected from streams and rivers. These assays will aid in the detection, monitoring, management, and conservation of these threatened species.

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