Detecting the brook floater, a freshwater mussel species at risk, using environmental DNA

Author(s):  
F. LeBlanc ◽  
R. Steeves ◽  
V. Belliveau ◽  
F. Akaishi ◽  
N. Gagné
Genome ◽  
2021 ◽  
pp. 1-12
Author(s):  
Scott A. Tarof ◽  
Steven Crookes ◽  
Kelsey Moxley ◽  
Jeff Hathaway ◽  
Graham Cameron ◽  
...  

Environmental DNA (eDNA) is gaining traction in conservation ecology as a powerful tool for detecting species at risk. We developed a quantitative polymerase chain reaction assay to detect a DNA amplicon fragment of the mitochondrial nicotinamide adenine dinucleotide locus of the Blanding’s turtle (Emydoidea blandingii) for detecting overwintering individuals. Seventy-eight water samples were collected from 17 wetland sites in Ontario, Canada. We used traditional field data to identify a priori positive and negative control sites. Fifty percent of positive control sites amplified. Detection was related to the number of individuals estimated from field observations in at least one region surveyed. Positive control sites had lower total dissolved solids and electrical conductivity in relation to negative control sites. Shedding rates were within the same order of magnitude for brumating and active turtles. We recommend collecting additional samples at a larger number of locations to maximize detection. Recommended sampling design changes may overshadow the additional effects of water chemistry and low eDNA shedding rates. eDNA offers tremendous potential to practitioners conducting species at risk assessments in environmental consulting by providing a faster, more efficient method of detection compared with traditional surveys.


2020 ◽  
Author(s):  
Torrey W. Rodgers ◽  
Joseph C. Dysthe ◽  
Cynthia Tait ◽  
Thomas W. Franklin ◽  
Michael K. Schwartz ◽  
...  

AbstractWe developed multiplexed, species-specific, quantitative PCR assays for the detection of four freshwater mussel species native to western North America, Gonidea angulata, Margaritifera falcata, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA (eDNA). These species have experienced dramatic declines over the last century and are currently threatened in many portions of their ranges. Therefore, improved tools for detecting and monitoring these species are needed. Species-specificity and sensitivity of assays were empirically tested in the lab, and multiplex assays were also validated with field collected eDNA samples. All assays were species-specific, sensitive, and effective for detection from eDNA samples collected from streams and rivers. These assays will aid in the detection, monitoring, management, and conservation of these vulnerable species.


2018 ◽  
Vol 28 (3) ◽  
pp. 545-558 ◽  
Author(s):  
Charise A. Currier ◽  
Todd J. Morris ◽  
Chris C. Wilson ◽  
Joanna R. Freeland

2020 ◽  
Vol 39 (4) ◽  
pp. 762-772
Author(s):  
Torrey W. Rodgers ◽  
Joseph C. Dysthe ◽  
Cynthia Tait ◽  
Thomas W. Franklin ◽  
Michael K. Schwartz ◽  
...  

2020 ◽  
Vol 134 (2) ◽  
pp. 179-188
Author(s):  
Todd J. Morris ◽  
Margaret N. Sheldon ◽  
Kelly A. McNichols-O'Rourke

Freshwater mussels of the family Unionidae are among the world’s most imperilled animals. A third of Canadian species have been assessed by the Committee on the Status of Endangered Wildlife in Canada as Extirpated, Endangered, Threatened, or Special Concern, with losses attributed to natural system modifications such as damming, pollution, exploitation for buttons and pearls, urbanization, and the introduction and subsequent effects of aquatic invasive species. In the Great Lakes basin, the introduction of dreissenid mussels in the 1980s caused catastrophic declines, with remnant populations restricted to lotic riverine habitats. In southwestern Ontario, the Canard River is the largest remaining direct tributary of the Detroit River that could provide a source of mussels to aid natural recovery. In 2019, nine sites in the Canard River were sampled using a timed-search approach (4.5 person-hours/site) with a combination of tactile searching by hand and mussel scoops (7-mm mesh) or underwater viewers. The search yielded 362 individuals of eight species, including two Species at Risk, Mapleleaf (Quadrula quadrula) and Lilliput (Toxolasma parvum), which had never been previously recorded in the Canard River.


2018 ◽  
Author(s):  
Torrey W. Rodgers ◽  
Karen E. Mock

AbstractWe developed species-specific quantitative PCR assays for the detection of two freshwater mussel species native to the western North America, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA. These species have experienced dramatic declines over the last century, and are currently threatened in many portions of their range. Improved tools for detecting and monitoring these species are needed. Species-specificity and sensitivity of the assays was empirically tested in the lab, and both assays were also validated with field collected eDNA samples. We found that the assays we designed are species-specific, sensitive, and are effective for detecting Anodonta nuttalliana and Anodonta oregonensis from environmental DNA samples collected from streams and rivers. These assays will aid in the detection, monitoring, management, and conservation of these threatened species.


2021 ◽  
Vol 108 (2) ◽  
Author(s):  
Jaroslav Koleček ◽  
Jiří Reif ◽  
Miroslav Šálek ◽  
Jan Hanzelka ◽  
Camille Sottas ◽  
...  

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