Application of HAADF STEM image analysis to structure determination in rotationally disordered and amorphous multilayered films

2016 ◽  
Vol 31 (8) ◽  
pp. 084003 ◽  
Author(s):  
Gavin Mitchson ◽  
Jeffrey Ditto ◽  
Keenan N Woods ◽  
Richard Westover ◽  
Catherine J Page ◽  
...  
Keyword(s):  
Image Analysis ◽  
Structure Determination ◽  
Multilayered Films ◽  
Stem Image

scholarly journals A resolution record for cryoEM

2021 ◽  
Vol 10 ◽  
Author(s):  
Jonathan Ashmore ◽  
Bridget Carragher ◽  
Peter B Rosenthal ◽  
William Weis
Keyword(s):  
Electron Microscopy ◽  
Image Analysis ◽  
Structure Determination ◽  
Test Specimen ◽  
Structural Information ◽  
Atomic Resolution ◽  
Fast Growing ◽  
Cryo Electron Microscopy ◽  
New Instrument ◽  
Resolution Structure

Cryo electron microscopy (cryoEM) is a fast-growing technique for structure determination. Two recent papers report the first atomic resolution structure of a protein obtained by averaging images of frozen-hydrated biomolecules. They both describe maps of symmetric apoferritin assemblies, a common test specimen, in unprecedented detail. New instrument improvements, different in the two studies, have contributed better images, and image analysis can extract structural information sufficient to resolve individual atomic positions. While true atomic resolution maps will not be routine for most proteins, the studies suggest structures determined by cryoEM will continue to improve, increasing their impact on biology and medicine.

HAADF‐STEM image analysis for size‐selected platinum nanoclusters

2020 ◽  
Vol 279 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Y. XIA ◽  
P. HARRISON ◽  
I.M. ORNELAS ◽  
H.L. WANG ◽  
Z.Y. LI
Keyword(s):  
Image Analysis ◽  
Stem Image ◽  
Platinum Nanoclusters

Quantitative HAADF-STEM image analysis using IMAGE-WARP processing

2003 ◽  
Vol 9 (S03) ◽  
pp. 52-53
Author(s):  
Aleksander Rečnik ◽  
Günter Möbus ◽  
Sašo Šturm
Keyword(s):  
Image Analysis ◽  
Stem Image ◽  
Warp Processing

Reproducibility of CBCT image analysis: a clinical study on intrapersonal and interpersonal errors in bone structure determination

2018 ◽  
Vol 35 (2) ◽  
pp. 152-158
Author(s):  
Sigmar Schnutenhaus ◽  
Michael Graf ◽  
Isabel Doering ◽  
Ralph G. Luthardt ◽  
Heike Rudolph
Keyword(s):  
Image Analysis ◽  
Clinical Study ◽  
Structure Determination ◽  
Bone Structure ◽  
Cbct Image

STEM Image Analysis Using LAT Image Processing

2009 ◽  
Vol 11 (3) ◽  
pp. 34-38 ◽  
Author(s):  
Petra Bele ◽  
Ulrich Stimming ◽  
Hiroshi Yano ◽  
Hiroyuki Uchida ◽  
Masahiro Watanabe
Keyword(s):  
Image Processing ◽  
Image Analysis ◽  
Stem Image

HREM Image Analysis up to Structure Determination of SbCrSe3: A New 1D Ferromagnet

1997 ◽  
Vol 132 (2) ◽  
pp. 257-266 ◽  
Author(s):  
V.V. Volkov ◽  
G. Van Tendeloo ◽  
J. Van Landuyt ◽  
S. Amelinckx ◽  
E.E. Busheva ◽  
...  
Keyword(s):  
Image Analysis ◽  
Structure Determination ◽  
Hrem Image

Automated Image Analysis Of Nuclear And Cytoplasmic Changes In Exfoliated Cells From Tracheas Treated With Carcinogen

1977 ◽  
Vol 35 ◽  
pp. 220-221
Author(s):  
S.F. Stinson ◽  
J.C. Lilga ◽  
M.B. Sporn
Keyword(s):  
Image Analysis ◽  
Pattern Analysis ◽  
Relative Proportion ◽  
Automated Image Analysis ◽  
Image Analyzer ◽  
Cross Sectional ◽  
Exfoliated Cells ◽  
Neoplastic Lesions ◽  
Analysis System ◽  
Morphologic Criterion

Increased nuclear size, resulting in an increase in the relative proportion of nuclear to cytoplasmic sizes, is an important morphologic criterion for the evaluation of neoplastic and pre-neoplastic cells. This paper describes investigations into the suitability of automated image analysis for quantitating changes in nuclear and cytoplasmic cross-sectional areas in exfoliated cells from tracheas treated with carcinogen.Neoplastic and pre-neoplastic lesions were induced in the tracheas of Syrian hamsters with the carcinogen N-methyl-N-nitrosourea. Cytology samples were collected intra-tracheally with a specially designed catheter (1) and stained by a modified Papanicolaou technique. Three cytology specimens were selected from animals with normal tracheas, 3 from animals with dysplastic changes, and 3 from animals with epidermoid carcinoma. One hundred randomly selected cells on each slide were analyzed with a Bausch and Lomb Pattern Analysis System automated image analyzer.

Progress In The Practical Application Of Automated Image Analysis To Tem Negatives

1977 ◽  
Vol 35 ◽  
pp. 214-217
Author(s):  
F. A. Heckman ◽  
E. Redman ◽  
J.E. Connolly
Keyword(s):  
Image Analysis ◽  
Image Quality ◽  
Exposure Time ◽  
Image Contrast ◽  
Automated Image Analysis ◽  
Practical Application ◽  
Factors Affecting ◽  
High Image Quality ◽  
Qualitative Evidence ◽  
Comprehensive Study

In our initial publication on this subject1) we reported results demonstrating that contrast is the most important factor in producing the high image quality required for reliable image analysis. We also listed the factors which enhance contrast in order of the experimentally determined magnitude of their effect. The two most powerful factors affecting image contrast attainable with sheet film are beam intensity and KV. At that time we had only qualitative evidence for the ranking of enhancing factors. Later we carried out the densitometric measurements which led to the results outlined below.Meaningful evaluations of the cause-effect relationships among the considerable number of variables in preparing EM negatives depend on doing things in a systematic way, varying only one parameter at a time. Unless otherwise noted, we adhered to the following procedure evolved during our comprehensive study:Philips EM-300; 30μ objective aperature; magnification 7000- 12000X, exposure time 1 second, anti-contamination device operating.

A New System For Optomanual Semiautomatic Quantitative Image Analysis

1977 ◽  
Vol 35 ◽  
pp. 210-211
Author(s):  
H.P. Rohr
Keyword(s):  
Image Analysis ◽  
Volume Density ◽  
List Type ◽  
Distribution Analysis ◽  
Type Number ◽  
Point Counting ◽  
Human Eye ◽  
Quantitative Image ◽  
Point Density ◽  
Electronic Counting

Today, in image analysis the broadest possible rationalization and economization have become desirable. Basically, there are two approaches for image analysis: The image analysis through the so-called scanning methods which are usually performed without the human eye and the systems of optical semiautomatic analysis completely relying on the human eye.The new MOP AM 01 opto-manual system (fig.) represents one of the very promising approaches in this field. The instrument consists of an electronic counting and storing unit, which incorporates a microprocessor and a keyboard for choice of measuring parameters, well designed for easy use.Using the MOP AM 01 there are three possibilities of image analysis:the manual point counting,the opto-manual point counting andthe measurement of absolute areas and/or length (size distribution analysis included).To determine a point density for the calculation of the corresponding volume density the intercepts lying within the structure are scanned with the light pen.

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