scholarly journals The addition of various carbon sources on growing media to increase the siderophore level of fluorescent pseudomonad bacteria

2019 ◽  
Vol 1317 ◽  
pp. 012078
Author(s):  
Linda Advinda ◽  
Ilham Pratama ◽  
Mades Fifendy ◽  
Azwir Anhar ◽  
Armaleni
1997 ◽  
Vol 60 (12) ◽  
pp. 1515-1519 ◽  
Author(s):  
CHRIS W. MICHIELS ◽  
MIA SCHELLEKENS ◽  
CARINE C. F. SOONTJENS ◽  
KRISTEL J. A. HAUBEN

Nine spots on a meat mincer in a large catering facility were sampled four times over a three-month period after routine cleaning and disinfection. Spot 2 carried a significantly higher average microbial load than the other spots and was the only spot with a persistent population of fluorescent pseudomonads. The predominant type of fluorescent pseudomonads produced a yellow diffusible pigment and were always found in turkey meat. Since turkey was also the last type of meat minced on each working day, it was considered the likely source of these bacteria on the mincer. Sixty-three yellow-fluorescing pseudomonad strains from un-minced turkey and 17 strains from mincer spot 2 after mincing, cleaning, and disinfection were subjected to fingerprint analysis by random amplification of polymorphic DNA (RAPD) and by analysis of the assimilation patterns of 95 carbon sources using the Biolog system. Cluster analysis of the RAPD and the metabolic fingerprints revealed that all the mincer strains formed a homogeneous cluster, exclusive of all the turkey strains which displayed a higher degree of heterogeneity. Six strains isolated 1 week earlier and six strains isolated 11 weeks earlier from spot 2 were also subjected to RAPD analysis. Their fingerprints fell within the existing cluster of 17 spot 2 strains. These results suggest the existence on mincer spot 2 of an endemic flora of fluorescent pseudomonads, probably in the form of a biofilm. The genetic and physiological homogeneity of this flora, as opposed to the heterogeneity of the raw meat flora, indicates that only a fraction of the raw meat population of P. fiuorescens is successful in forming biofilm in this environment.


BioScience ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 50
Author(s):  
Ilham Pratama ◽  
Linda Advinda ◽  
Mades Fifendy

BioScience ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 65
Author(s):  
Ansel Nastika ◽  
V Violita ◽  
Irma Laelani Eka Putri

Recent years have seen growing media and political attention to the issue of tourism and crime in a number of countries. Issues such as drugs tourism, sex tourism & alcohol-related crime and disorder have highlighted crimes and rule-breaking by tourists.


1999 ◽  
Vol 17 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Robert H. Stamps ◽  
Michael R. Evans

Abstract A comparison was made of Canadian sphagnum peat (SP) and Philippine coconut (Cocos nucifera L.) coir dust (CD) as growing media components for greenhouse production of Dracaena marginata Bak. and Spathiphyllum Schott ‘Petite’. Three soilless foliage plant growing mixes (Cornell, Hybrid, University of Florida #2 [UF-2]) were prepared using either SP or CD and pine bark (PB), vermiculite (V), and/or perlite (P) in the following ratios (% by vol): Cornell = 50 CD or SP:25 V:25 P, Hybrid = 40 CD or SP:30 V:30 PB, UF-2 = 50 CD or SP: 50 PB. Dracaena root growth was not affected by treatments but there were significant mix × media component interactions that affected plant top growth parameters. In general, the growth and quality of D. marginata were reduced by using CD in Cornell, had no effect in Hybrid, and increased in UF-2. S. ‘Petite’ grew equally well in all growing mixes regardless of whether CD or SP was used; however, plants grew more in Cornell and Hybrid than in UF-2. S. ‘Petite’ roots, which were infested with Cylindrocladium spathiphylli, had higher grades when grown in CD than when the media contained SP.


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