Adipogenic differentiation of scaffold-bound human adipose tissue-derived stem cells (hASC) for soft tissue engineering

2012 ◽  
Vol 7 (5) ◽  
pp. 054107 ◽  
Author(s):  
M Handel ◽  
T R Hammer ◽  
D Hoefer
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Adipogenic Differentiation ◽  
Human Adipose Tissue ◽  
Soft Tissue Engineering

Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells

Biomaterials ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 1914-1923 ◽  
Author(s):  
Hoi Ki Cheung ◽  
Tim Tian Y. Han ◽  
Dale M. Marecak ◽  
John F. Watkins ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Hydrogel Scaffolds ◽  
Soft Tissue Engineering

scholarly journals Methacrylated gelatin/hyaluronan-based hydrogels for soft tissue engineering

2017 ◽  
Vol 8 ◽  
pp. 204173141774415 ◽  
Author(s):  
Lukas Kessler ◽  
Sandra Gehrke ◽  
Marc Winnefeld ◽  
Birgit Huber ◽  
Eva Hoch ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Adipogenic Differentiation ◽  
Building Blocks ◽  
Fatty Acid Binding ◽  
Angiogenic Potential ◽  
Adipose Tissue Engineering ◽  
Angiogenesis Assay ◽  
Soft Tissue Engineering

In vitro–generated soft tissue could provide alternate therapies for soft tissue defects. The aim of this study was to evaluate methacrylated gelatin/hyaluronan as scaffolds for soft tissue engineering and their interaction with human adipose–derived stem cells (hASCs). ASCs were incorporated into methacrylated gelatin/hyaluronan hydrogels. The gels were photocrosslinked with a lithium phenyl-2,4,6-trimethylbenzoylphosphinate photoinitiator and analyzed for cell viability and adipogenic differentiation of ASCs over a period of 30 days. Additionally, an angiogenesis assay was performed to assess their angiogenic potential. After 24 h, ASCs showed increased viability on composite hydrogels. These results were consistent over 21 days of culture. By induction of adipogenic differentiation, the mature adipocytes were observed after 7 days of culture, their number significantly increased until day 28 as well as expression of fatty acid binding protein 4 and adiponectin. Our scaffolds are promising as building blocks for adipose tissue engineering and allowed long viability, proliferation, and differentiation of ASCs.

scholarly journals Wnt Antagonist Secreted Frizzled-Related Protein 4 Upregulates Adipogenic Differentiation in Human Adipose Tissue-Derived Mesenchymal Stem Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0118005 ◽  
Author(s):  
Malini Visweswaran ◽  
Luca Schiefer ◽  
Frank Arfuso ◽  
Rodney J. Dilley ◽  
Philip Newsholme ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Adipogenic Differentiation ◽  
Human Adipose Tissue ◽  
Related Protein
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