Polymorphism parental survey in three Indonesia improved varieties to support development of new submergence tolerant varieties
Abstract Submergence by flooding caused damage in rice growing areas and huge economic loss, and developing tolerant varieties is considered as the best approach to overcome the problem. Markers Assisted Backcrossing (MABC) approach is widely to develop Sub-1 tolerant varieties. The availability of polymorphic markers is among the most crucial requirement to implement the MABC method. This research was subjected to assess DNA polymorphism between IR64Sub1 and tree Indonesian popular varieties. A total of 136 microsatellites/simple sequence repeat markers were used to genotype tree Indonesian popular varieties; Cisantana, Angke and Mekongga and IR64-Sub1. A total of 39 markers covering 11 chromosomes were found polymorphic between IR64 Sub-1 and the three varieties, however no polymorphic markers found in chromosome 12. The lack polymorphic markers were also found in chromosome 10 and 11 between IR64 Sub 1 and Angke. With the completion of the missing markers, these 39 polymorphic SSR markers can be utilized to support the MABC program for the development of new Sub-1 tolerant variety with multiple tolerances.
