In order to improve the dynamic stability of precision micro slitting turn-milling machine tools, reduce or avoid the vibration problem during the cutting process, optimize the machine structure and processing parameters, the modal analysis of precision micro slitting turn-milling machine tool based on hammer experimental method was researched. In this paper, by analyzing the mechanism of precision micro slitting turn-milling machine tools, the multi degree-of-freedom mathematical vibration model of precision slitting turn-milling machine tools is constructed. The precision micro turn-milling machine tool is analyzed based on the hammer experiment analysis. The modal analysis obtained the first five natural frequencies and resonance speeds of the precision micro slitting turn-milling machine tool,including ST26, NN-25UB8K2 and NN-20UB87. The research results show that hammer experimental method can evaluate the vibration modal analysis of precision micro slitting turn-milling machine tools to some extent. The experimental modal analysis results guide and optimize the structural design and processing technology of precision micro slitting turn-milling machine tools.