Development and Evaluation of a Versatile Receptor-Ligand Binding Assay Using Cell Membrane Preparations Embedded in an Agarose Gel Matrix and Evaluation with the Human Adenosine A1 Receptor

Dirk Bier; Annette Schulze; Marcus Holschbach; Bernd Neumaier; Arnd Baumann

doi:10.1089/adt.2020.991

Development and Evaluation of a Versatile Receptor-Ligand Binding Assay Using Cell Membrane Preparations Embedded in an Agarose Gel Matrix and Evaluation with the Human Adenosine A1 Receptor

Assay and Drug Development Technologies ◽

10.1089/adt.2020.991 ◽

2020 ◽

Vol 18 (7) ◽

pp. 328-340

Author(s):

Dirk Bier ◽

Annette Schulze ◽

Marcus Holschbach ◽

Bernd Neumaier ◽

Arnd Baumann

Keyword(s):

Cell Membrane ◽

Ligand Binding ◽

Binding Assay ◽

Adenosine A1 Receptor ◽

Agarose Gel ◽

Ligand Binding Assay ◽

Receptor Ligand ◽

Adenosine A1 ◽

A1 Receptor

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First no-carrier-added radioselenation of an adenosine-A1 receptor ligand

Journal of Labelled Compounds and Radiopharmaceuticals ◽

10.1002/jlcr.829 ◽

2004 ◽

Vol 47 (7) ◽

pp. 415-427 ◽

Cited By ~ 6

Author(s):

Till Blum ◽

Johannes Ermert ◽

Walter Wutz ◽

Dirk Bier ◽

Heinz H. Coenen

Keyword(s):

Adenosine A1 Receptor ◽

Receptor Ligand ◽

Adenosine A1 ◽

A1 Receptor ◽

No Carrier Added

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A Homogeneous G Protein-Coupled Receptor Ligand Binding Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer

Assay and Drug Development Technologies ◽

10.1089/adt.2008.152 ◽

2008 ◽

Vol 6 (4) ◽

pp. 543-550 ◽

Cited By ~ 22

Author(s):

Liaoyuan A. Hu ◽

Tian Zhou ◽

Brian D. Hamman ◽

Qingyun Liu

Keyword(s):

Energy Transfer ◽

Ligand Binding ◽

Binding Assay ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Ligand Binding Assay ◽

Time Resolved Fluorescence ◽

Receptor Ligand ◽

Time Resolved ◽

G Protein Coupled

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Naturally occurring benzofuran: isolation, structure elucidation and total synthesis of 5-(3-hydroxypropyl)-7-methoxy-2-(3′-methoxy-4′-hydroxyphenyl)-3-benzo[b]furancarbaldehyde, a novel adenosine A1 receptor ligand isolated from salvia miltiorrhiza bunge (danshen)

Tetrahedron Letters ◽

10.1016/s0040-4039(00)78908-8 ◽

1991 ◽

Vol 32 (18) ◽

pp. 2061-2064 ◽

Cited By ~ 46

Author(s):

Zhen Yang ◽

Po Ming Hon ◽

Kuk Ying Chui ◽

Zun Le Xu ◽

Hson Mou Chang ◽

...

Keyword(s):

Total Synthesis ◽

Structure Elucidation ◽

Salvia Miltiorrhiza ◽

Adenosine A1 Receptor ◽

Salvia Miltiorrhiza Bunge ◽

Receptor Ligand ◽

Naturally Occurring ◽

Adenosine A1 ◽

A1 Receptor

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Evaluation of carbon-11 labeled KF15372 and its ethyl and methyl derivatives as a potential CNS adenosine A1 receptor ligand

Nuclear Medicine and Biology ◽

10.1016/s0969-8051(96)00161-8 ◽

1997 ◽

Vol 24 (1) ◽

pp. 53-59 ◽

Cited By ~ 34

Author(s):

Junko Noguchi ◽

Kiichi Ishiwata ◽

Riko Furuta ◽

Jun-ichi Simada ◽

Motohiro Kiyosawa ◽

...

Keyword(s):

Adenosine A1 Receptor ◽

Receptor Ligand ◽

Adenosine A1 ◽

A1 Receptor ◽

Methyl Derivatives

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ChemInform Abstract: Naturally Occurring Benzofuran: Isolation, Structure Elucidation, and Total Synthesis of 5-(3-Hydroxypropyl)-7-methoxy-2-(3′-methoxy-4′- hydroxyphenyl)-3-benzo(b)furancarbaldehyde, a Novel Adenosine A1 Receptor Ligand Isolated from Sa

ChemInform ◽

10.1002/chin.199206166 ◽

2010 ◽

Vol 23 (6) ◽

pp. no-no ◽

Cited By ~ 1

Author(s):

Z. YANG ◽

P. M. HON ◽

K. Y. CHUI ◽

Z. L. XU ◽

H. M. CHANG ◽

...

Keyword(s):

Total Synthesis ◽

Structure Elucidation ◽

Adenosine A1 Receptor ◽

Receptor Ligand ◽

Naturally Occurring ◽

Adenosine A1 ◽

A1 Receptor

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Cell-based β2-adrenergic receptor–ligand binding assay using synthesized europium-labeled ligands and time-resolved fluorescence

Analytical Biochemistry ◽

10.1016/j.ab.2009.05.022 ◽

2009 ◽

Vol 392 (2) ◽

pp. 103-109 ◽

Cited By ~ 12

Author(s):

Eija Martikkala ◽

Mirva Lehmusto ◽

Minna Lilja ◽

Anita Rozwandowicz-Jansen ◽

Jenni Lunden ◽

...

Keyword(s):

Ligand Binding ◽

Adrenergic Receptor ◽

Binding Assay ◽

Ligand Binding Assay ◽

Time Resolved Fluorescence ◽

Receptor Ligand ◽

Time Resolved ◽

Β2 Adrenergic Receptor

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Development of a Nonradioactive Steroid Receptor Ligand Binding Assay Using Fluorescence Polarization for Studying Hsp90‐Mediated Chaperone Protein Activity

The FASEB Journal ◽

10.1096/fasebj.29.1_supplement.716.17 ◽

2015 ◽

Vol 29 (S1) ◽

Author(s):

Nicholas Manlove ◽

Jane Walden ◽

Patrick Murphy

Keyword(s):

Ligand Binding ◽

Fluorescence Polarization ◽

Binding Assay ◽

Steroid Receptor ◽

Ligand Binding Assay ◽

Protein Activity ◽

Receptor Ligand ◽

Chaperone Protein

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ChemInform Abstract: Regioselective Introduction of Carbon-3 Substituents to 5-Alkyl-7- methoxy-2-phenylbenzo(b)furans: Synthesis of a Novel Adenosine A1 Receptor Ligand and Its Derivatives.

ChemInform ◽

10.1002/chin.199320175 ◽

2010 ◽

Vol 24 (20) ◽

pp. no-no

Author(s):

Z. YANG ◽

H. B. LIU ◽

C. M. LEE ◽

H. M. CHANG ◽

H. N. C. WONG

Keyword(s):

Adenosine A1 Receptor ◽

Receptor Ligand ◽

Adenosine A1 ◽

A1 Receptor

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A Chemiluminescence Based Receptor-ligand Binding Assay Using Peptide Ligands with an Acridinium Ester Label

BIO-PROTOCOL ◽

10.21769/bioprotoc.1422 ◽

2015 ◽

Vol 5 (6) ◽

Cited By ~ 1

Author(s):

Mari Wildhagen ◽

Melinka Butenko ◽

Reidunn Aalen ◽

Georg Felix ◽

Markus Albert

Keyword(s):

Ligand Binding ◽

Binding Assay ◽

Peptide Ligands ◽

Ligand Binding Assay ◽

Receptor Ligand ◽

Acridinium Ester

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Compounds from Danshen. Part 7. Regioselective introduction of carbon-3 substituents to 5-alkyl-7-methoxy-2-phenylbenzo[b]furans: synthesis of a novel adenosine A1 receptor ligand and its derivatives

The Journal of Organic Chemistry ◽

10.1021/jo00052a046 ◽

1992 ◽

Vol 57 (26) ◽

pp. 7248-7257 ◽

Cited By ~ 64

Author(s):

Zhen Yang ◽

Han Biao Liu ◽

Chi Ming Lee ◽

Hson Mou Chang ◽

Henry N. C. Wong

Keyword(s):

Adenosine A1 Receptor ◽

Receptor Ligand ◽

Adenosine A1 ◽

A1 Receptor

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