scholarly journals Development of a Nonradioactive Steroid Receptor Ligand Binding Assay Using Fluorescence Polarization for Studying Hsp90‐Mediated Chaperone Protein Activity

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Nicholas Manlove ◽  
Jane Walden ◽  
Patrick Murphy
Keyword(s):  
Ligand Binding ◽  
Fluorescence Polarization ◽  
Binding Assay ◽  
Steroid Receptor ◽  
Ligand Binding Assay ◽  
Protein Activity ◽  
Receptor Ligand ◽  
Chaperone Protein

scholarly journals Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay

2005 ◽  
Vol 10 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Marko E. Tirri ◽  
Roope J. Huttunen ◽  
Juha Toivonen ◽  
Pirkko L. Härkönen ◽  
Juhani T. Soini ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Fluorescence Polarization ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Lead Compounds ◽  
Two Photon ◽  
Photon Excitation ◽  
Homogeneous Assay ◽  
Estrogen Receptor Ligand

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

Cell-based β2-adrenergic receptor–ligand binding assay using synthesized europium-labeled ligands and time-resolved fluorescence

2009 ◽  
Vol 392 (2) ◽  
pp. 103-109 ◽  
Author(s):  
Eija Martikkala ◽  
Mirva Lehmusto ◽  
Minna Lilja ◽  
Anita Rozwandowicz-Jansen ◽  
Jenni Lunden ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Adrenergic Receptor ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Time Resolved Fluorescence ◽  
Receptor Ligand ◽  
Time Resolved ◽  
Β2 Adrenergic Receptor

A Chemiluminescence Based Receptor-ligand Binding Assay Using Peptide Ligands with an Acridinium Ester Label

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (6) ◽  
Author(s):  
Mari Wildhagen ◽  
Melinka Butenko ◽  
Reidunn Aalen ◽  
Georg Felix ◽  
Markus Albert
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Peptide Ligands ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Acridinium Ester

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand
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