Molecular Interaction Between Nitric Oxide and Ryanodine Receptors of Skeletal and Cardiac Sarcoplasmic Reticulum

In the present study, effects of the nitric oxide donor, S-nitroso-N-acetylpenicillamine (SNAP), on sarcoplasmic reticulum (SR) Ca2+ release were examined in freshly dissociated porcine tracheal smooth muscle (TSM) cells. Fura 2-loaded TSM cells were imaged using video fluorescence microscopy. SR Ca2+ release was induced by acetylcholine (ACh), which acts principally through inositol 1,4,5-trisphosphate (IP3) receptors, and by caffeine, which acts principally through ryanodine receptors (RyR). SNAP inhibited ACh-induced SR Ca2+ release at both 0 and 2.5 mM extracellular Ca2+. Degraded SNAP had no effect on ACh-induced SR Ca2+ release. SNAP also inhibited caffeine-induced SR Ca2+ release. ACh-induced Ca2+ influx was not affected by SNAP when SR reloading was blocked by thapsigargin. SNAP also did not affect SR Ca2+ reuptake. The membrane-permeant analogue of guanosine 3',5'-cyclic monophosphate (cGMP), 8-bromo-cGMP, mimicked the effects of SNAP. These results suggest that, in porcine TSM cells, SNAP reduces the intracellular Ca2+ response to ACh and caffeine by inhibiting SR Ca2+ release through both IP3 and RyR, but not by inhibiting influx or repletion of the SR Ca2+ stores. These effects are likely mediated via cGMP-dependent mechanisms.

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Nitric Oxide Prevents Myoglobin/tert-Butyl Hydroperoxide-Induced Inhibition of Ca2+ Transport in Skeletal and Cardiac Sarcoplasmic Reticulum

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1999.tb09252.x ◽

1999 ◽

Vol 874 (1 HEART IN STRE) ◽

pp. 371-385 ◽

Cited By ~ 3

Author(s):

ELIZABETH V. MENSHIKOVA ◽

VLADIMIR B. RITOV ◽

NIKOLAJ V. GORBUNOV ◽

GUY SALAMA ◽

H. GREGG CLAYCAMP ◽

...

Keyword(s):

Nitric Oxide ◽

Sarcoplasmic Reticulum ◽

Cardiac Sarcoplasmic Reticulum ◽

Butyl Hydroperoxide ◽

Tert Butyl ◽

Tert Butyl Hydroperoxide

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Alterations in cardiac sarcoplasmic reticulum Ca 2+ releasing channels (ryanodine receptors) of the atrial myocardium in patients with atrial fibrillation

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(98)80180-0 ◽

1998 ◽

Vol 31 ◽

pp. 422

Author(s):

T. Ohkusa ◽

T. Ueyama ◽

M. Yano ◽

Y. Fujumura ◽

K. Eeato ◽

...

Keyword(s):

Atrial Fibrillation ◽

Sarcoplasmic Reticulum ◽

Ryanodine Receptors ◽

Atrial Myocardium ◽

Cardiac Sarcoplasmic Reticulum

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Nitric oxide synthase in cardiac sarcoplasmic reticulum

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.96.2.657 ◽

1999 ◽

Vol 96 (2) ◽

pp. 657-662 ◽

Cited By ~ 343

Author(s):

K. Y. Xu ◽

D. L. Huso ◽

T. M. Dawson ◽

D. S. Bredt ◽

L. C. Becker

Keyword(s):

Nitric Oxide ◽

Sarcoplasmic Reticulum ◽

Nitric Oxide Synthase ◽

Cardiac Sarcoplasmic Reticulum ◽

Oxide Synthase

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A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051086 ◽

1974 ◽

Vol 32 ◽

pp. 214-215

Author(s):

R. A. Waugh ◽

J. R. Sommer

Keyword(s):

Complex System ◽

Electron Microscope ◽

Sarcoplasmic Reticulum ◽

Transmission Electron Microscope ◽

Electron Microscopic ◽

Cardiac Sarcoplasmic Reticulum ◽

Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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