scholarly journals Molecular Interaction Between Nitric Oxide and Ryanodine Receptors of Skeletal and Cardiac Sarcoplasmic Reticulum

2000 ◽  
Vol 2 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Guy Salama ◽  
Elizaveta V. Menshikova ◽  
Jonathan J. Abramson
Cell Calcium ◽  
2005 ◽  
Vol 37 (1) ◽  
pp. 87-96 ◽  
Author(s):  
Eunji Cheong ◽  
Vassil Tumbev ◽  
Jon Abramson ◽  
Guy Salama ◽  
Detcho A. Stoyanovsky

1997 ◽  
Vol 272 (1) ◽  
pp. L1-L7 ◽  
Author(s):  
M. S. Kannan ◽  
Y. S. Prakash ◽  
D. E. Johnson ◽  
G. C. Sieck

In the present study, effects of the nitric oxide donor, S-nitroso-N-acetylpenicillamine (SNAP), on sarcoplasmic reticulum (SR) Ca2+ release were examined in freshly dissociated porcine tracheal smooth muscle (TSM) cells. Fura 2-loaded TSM cells were imaged using video fluorescence microscopy. SR Ca2+ release was induced by acetylcholine (ACh), which acts principally through inositol 1,4,5-trisphosphate (IP3) receptors, and by caffeine, which acts principally through ryanodine receptors (RyR). SNAP inhibited ACh-induced SR Ca2+ release at both 0 and 2.5 mM extracellular Ca2+. Degraded SNAP had no effect on ACh-induced SR Ca2+ release. SNAP also inhibited caffeine-induced SR Ca2+ release. ACh-induced Ca2+ influx was not affected by SNAP when SR reloading was blocked by thapsigargin. SNAP also did not affect SR Ca2+ reuptake. The membrane-permeant analogue of guanosine 3',5'-cyclic monophosphate (cGMP), 8-bromo-cGMP, mimicked the effects of SNAP. These results suggest that, in porcine TSM cells, SNAP reduces the intracellular Ca2+ response to ACh and caffeine by inhibiting SR Ca2+ release through both IP3 and RyR, but not by inhibiting influx or repletion of the SR Ca2+ stores. These effects are likely mediated via cGMP-dependent mechanisms.


1999 ◽  
Vol 874 (1 HEART IN STRE) ◽  
pp. 371-385 ◽  
Author(s):  
ELIZABETH V. MENSHIKOVA ◽  
VLADIMIR B. RITOV ◽  
NIKOLAJ V. GORBUNOV ◽  
GUY SALAMA ◽  
H. GREGG CLAYCAMP ◽  
...  

1999 ◽  
Vol 96 (2) ◽  
pp. 657-662 ◽  
Author(s):  
K. Y. Xu ◽  
D. L. Huso ◽  
T. M. Dawson ◽  
D. S. Bredt ◽  
L. C. Becker

Author(s):  
R. A. Waugh ◽  
J. R. Sommer

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.


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