scholarly journals A Differential Pressure Laminar Flow Reactor Supports Osteogenic Differentiation and Extracellular Matrix Formation from Adipose Mesenchymal Stem Cells in a Macroporous Ceramic Scaffold

2012 ◽  
Vol 1 (3) ◽  
pp. 145-156 ◽  
Author(s):  
Birgit Weyand ◽  
Cornelia Kasper ◽  
Meir Israelowitz ◽  
Christoph Gille ◽  
Herbert P. von Schroeder ◽  
...  
2019 ◽  
Vol 20 (16) ◽  
pp. 4024
Author(s):  
Hsiou-Hsin Tsai ◽  
Kai-Chiang Yang ◽  
Meng-Huang Wu ◽  
Jung-Chih Chen ◽  
Ching-Li Tseng

The culture environment plays an important role for stem cells’ cultivation. Static or dynamic culture preserve differential potentials to affect human mesenchymal stem cells’ (hMSCs) proliferation and differentiation. In this study, hMSCs were seeded on fiber disks and cultured in a bidirectional-flow bioreactor or spinner-flask bioreactor with a supplement of osteogenic medium. The hMSCs’ proliferation, osteogenic differentiation, and extracellular matrix deposition of mineralization were demonstrated. The results showed that the spinner flask improved cell viability at the first two weeks while the bidirectional-flow reactor increased the cell proliferation of hMSCs through the four-week culture period. Despite the flow reactor having a higher cell number, a lower lactose/glucose ratio was noted, revealing that the bidirectional-flow bioreactor provides better oxygen accessibility to the cultured cells/disk construct. The changes of calcium ions in the medium, the depositions of Ca2+ in the cells/disk constructs, and alkaline phosphate/osteocalcin activities showed the static culture of hMSCs caused cells to mineralize faster than the other two bioreactors but without cell proliferation. Otherwise, cells were distributed uniformly with abundant extracellular matrix productions using the flow reactor. This reveals that the static and dynamic cultivations regulated the osteogenic process differently in hMSCs. The bidirectional-flow bioreactor can be used in the mass production and cultivation of hMSCs for applications in bone regenerative medicine.


2008 ◽  
Vol 27 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Benjamin Müller ◽  
Christian Prante ◽  
Martin Gastens ◽  
Joachim Kuhn ◽  
Knut Kleesiek ◽  
...  

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