scholarly journals A New Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis

2013 ◽  
Vol 20 (9) ◽  
pp. 703-711 ◽  
Author(s):  
Xiayu Rao ◽  
Dejian Lai ◽  
Xuelin Huang
Keyword(s):  
Polymerase Chain Reaction ◽  
Data Analysis ◽  
Real Time ◽  
New Method ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

scholarly journals Improved normalization of real-time reverse transcriptase polymerase chain reaction data using an external RNA control

2008 ◽  
Vol 376 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Stian Ellefsen ◽  
Kåre-Olav Stensløkken ◽  
Guro K. Sandvik ◽  
Tom A. Kristensen ◽  
Göran E. Nilsson
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

scholarly journals Automated Reverse Transcription Polymerase Chain Reaction Data Analysis for Sars-CoV-2 Detection

2021 ◽  
Author(s):  
Laura Gómez-Romero ◽  
Hugo Tovar ◽  
Joaquín Moreno-Contreras ◽  
Marco A. Espinoza ◽  
Guillermo de-Anda-Jáuregui
Keyword(s):  
Polymerase Chain Reaction ◽  
Data Analysis ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

NqA: An R-based algorithm for the normalization and analysis of microRNA quantitative real-time polymerase chain reaction data

2014 ◽  
Vol 461 ◽  
pp. 7-9 ◽  
Author(s):  
Paolo Verderio ◽  
Stefano Bottelli ◽  
Chiara Maura Ciniselli ◽  
Marco Alessandro Pierotti ◽  
Manuela Gariboldi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

scholarly journals System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Andrej-Nikolai Spiess ◽  
Stefan Rödiger ◽  
Michał Burdukiewicz ◽  
Thomas Volksdorf ◽  
Joel Tellinghuisen
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

scholarly journals Pitfalls in the normalization of real-time polymerase chain reaction data

2007 ◽  
Vol 102 (3) ◽  
pp. 195-197 ◽  
Author(s):  
M. C. Hendriks-Balk ◽  
M. C. Michel ◽  
A. E. Alewijnse
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Reaction Data ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Data

A New Method for FMR1 Gene Methylation Screening by Multiplex Methylation-Specific Real-Time Polymerase Chain Reaction

2011 ◽  
Vol 15 (6) ◽  
pp. 387-393 ◽  
Author(s):  
Marjanu Hikmah Elias ◽  
Ravindran Ankathil ◽  
Abdul Razak Salmi ◽  
Wanna Sudhikaran ◽  
Pornprot Limprasert ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
New Method ◽  
Fmr1 Gene ◽  
Gene Methylation ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Multiple Verticillium Species in Soil Using Density Flotation and Real-Time Polymerase Chain Reaction

Plant Disease ◽  
2011 ◽  
Vol 95 (12) ◽  
pp. 1571-1580 ◽  
Author(s):  
J. Debode ◽  
K. Van Poucke ◽  
S. C. França ◽  
M. Maes ◽  
M. Höfte ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Selective Medium ◽  
Soil Samples ◽  
New Method ◽  
Small Samples ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pcr Assays ◽  
Wet Sieving

Wet sieving of soil samples, followed by plating on semi-selective medium and microscopic analysis, is the most commonly used technique to quantify microsclerotia-forming Verticillium species in soil. However, the method is restricted to small samples, does not allow easy differentiation between species, and takes several weeks to complete. This study describes an alternative method to test 100-g soil samples for three Verticillium species (V. tricorpus, V. dahliae, and V. longisporum) using density flotation-based extraction of microsclerotia followed by new real-time polymerase chain reaction (PCR) assays. Primers for these real-time PCR assays were designed to the ribosomal DNA internal transcribed spacer for V. tricorpus and the β-tubulin gene for V. dahliae + V. longisporum and V. longisporum. Tests with artificially and naturally infested soils showed that the new method is reproducible and sensitive (0.1 to 0.5 microsclerotia/g soil), allows differentiation among the three species, and can be completed in one day. The results of the new method and the wet-sieving method were highly correlated for V. tricorpus (R2 = 0.78), but not for V. dahliae/V. longisporum, probably due to the loss of germinability of V. dahliae/V. longisporum microsclerotia during prolonged dry storage of the soil.

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