An NFIL-6 Sequence Near the Transcriptional Initiation Site Is Necessary for the Lipopolysaccharide Induction of Murine Interleukin-lβ

1994 ◽  
Vol 13 (6) ◽  
pp. 561-569 ◽  
Author(s):  
SANDIP A. GODAMBE ◽  
DAVID D. CHAPLIN ◽  
TSETSKA TAKOVA ◽  
CLIFFORD J. BELLONE
Keyword(s):  
Initiation Site ◽  
Transcriptional Initiation ◽  
Transcriptional Initiation Site

A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter

1990 ◽  
Vol 10 (12) ◽  
pp. 6709-6717
Author(s):  
L S Lum ◽  
L A Sultzman ◽  
R J Kaufman ◽  
D I Linzer ◽  
B J Wu
Keyword(s):  
Initiation Site ◽  
Dependent Manner ◽  
Hsp70 Gene ◽  
Hsp70 Promoter ◽  
Transcriptional Initiation ◽  
Enhancer Binding Protein ◽  
Binding Factor ◽  
Ccaat Box ◽  
Transcriptional Initiation Site ◽  
Human Hsp70

The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.

Precise assignment of the light-strand promoter of mouse mitochondrial DNA: a functional promoter consists of multiple upstream domains

1986 ◽  
Vol 6 (9) ◽  
pp. 3253-3261
Author(s):  
D D Chang ◽  
D A Clayton
Keyword(s):  
Mitochondrial Dna ◽  
Initiation Site ◽  
Upstream Region ◽  
Mitochondrial Rna ◽  
Base Pairs ◽  
Transcriptional Initiation ◽  
Mitochondrial Rna Polymerase ◽  
Template Dna ◽  
Light Strand ◽  
Transcriptional Initiation Site

Using deletion mutagenesis we localized the promoter for the light strand of mouse mitochondrial DNA to a 97-base-pair region, from -88 to +9 nucleotides of the transcriptional initiation site. Within this region the light-strand promoter could be dissected into at least three different functional domains. The specificity region, a maximum of 19 base pairs between -10 and +9 of the transcriptional initiation site, was essential and sufficient for accurate transcriptional initiation. A second region, extending to -29 nucleotides from the initiation site, facilitated the formation of a preinitiation complex between the template DNA and factor(s) present in the mitochondrial RNA polymerase fraction and was required for efficient transcription. A third, ill-defined upstream region, which extended up to -88 nucleotides from the initiation site, appeared to influence template transcriptional efficiencies in competition assays. Without the specificity domain, the upstream regions were incapable of supporting any transcription. The presence of multiple upstream domains was confirmed by disrupting nucleotide sequences in the upstream region by using linker insertion and linker replacement techniques.

scholarly journals A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter.

1990 ◽  
Vol 10 (12) ◽  
pp. 6709-6717 ◽  
Author(s):  
L S Lum ◽  
L A Sultzman ◽  
R J Kaufman ◽  
D I Linzer ◽  
B J Wu
Keyword(s):  
Initiation Site ◽  
Dependent Manner ◽  
Hsp70 Gene ◽  
Hsp70 Promoter ◽  
Transcriptional Initiation ◽  
Enhancer Binding Protein ◽  
Binding Factor ◽  
Ccaat Box ◽  
Transcriptional Initiation Site ◽  
Human Hsp70

The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.

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