Precise Characterization of the Epitope Recognized by a Monoclonal Antibody Against Escherichia coli RNA Polymerase

Hybridoma ◽  
2005 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Estelle Andre ◽  
Martine Pugniere ◽  
Jaqueline Latouche ◽  
Claude Granier ◽  
Jean-Paul Leonetti
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibody ◽  
Rna Polymerase ◽  
Precise Characterization

scholarly journals Promoter Interference in a Bacteriophage Lambda Control Region: Effects of a Range of Interpromoter Distances

2000 ◽  
Vol 182 (1) ◽  
pp. 216-220 ◽  
Author(s):  
Michael G. Strainic ◽  
Jennifer J. Sullivan ◽  
Julio Collado-Vides ◽  
Pieter L. deHaseth
Keyword(s):  
Escherichia Coli ◽  
Complex Formation ◽  
Rna Polymerase ◽  
Control Region ◽  
Bacteriophage Lambda ◽  
Detailed Characterization ◽  
Phosphodiester Bonds ◽  
Divergent Promoters ◽  
Polymerase Binding

ABSTRACT The pR and pRM promoters of bacteriophage lambda direct transcription in divergent directions from start sites separated by 83 phosphodiester bonds. We had previously shown that the presence of an RNA polymerase at pR interfered with open complex formation at pRM and that this effect was alleviated by the deletion of 10 bp between the two promoters. Here we present a detailed characterization of the dependence of the interference on the interpromoter distance. It was found that the reduced interference between the two promoters is unique to the 10-bp deletion. The relief of interference was demonstrated to be due to the facilitation of a step subsequent to RNA polymerase binding to the pRM promoter. A model to explain these observations is proposed. A search of known Escherichia coli promoters identified three pairs of divergent promoters with similar separations to those investigated here.

scholarly journals Characterization of double-stranded ribonucleic acid sequences present in the initial transcription products of rat liver chromatin

1977 ◽  
Vol 165 (2) ◽  
pp. 237-245 ◽  
Author(s):  
E Pays
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Rat Liver ◽  
Rna Sequences ◽  
Complementary Sequences ◽  
Exogenous Rna ◽  
Liver Chromatin ◽  
Low Ionic Strength

At low ionic strength and with a low exogenous RNA polymerase/DNA ratio, rat liver chromatin directs the synthesis in vitro of RNA sequences rich in double-stranded segments. All the transcripts contain at least one double-stranded sequence. Most of the double-stranded segments are formed by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. They are heterogeneous in size, 35-45% of them being more than 80 nucleotides long. They contain 61-64% G+C, whether synthesized by rat liver RNA polymerase (form B) or Escherichia coli RNA polymerase. The largest double-stranded sequences are found in the largest transcripts, and are the most thermostable. The fidelity of base-matching is better in double-stranded transcripts synthesized on rat liver chromatin by homologous polymerase than in those synthesized on it by a bacterial polymerase, or in those synthesized by either of the two polymerases on pure DNA.

scholarly journals Function-Based Selection and Characterization of Base-Pair Polymorphisms in a Promoter of Escherichia coli RNA Polymerase-ς70

2001 ◽  
Vol 183 (9) ◽  
pp. 2866-2873 ◽  
Author(s):  
Jian Xu ◽  
Barbara C. McCabe ◽  
Gerald B. Koudelka
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Promoter Sequence ◽  
Kinetic Properties ◽  
Optimal Sequence ◽  
Sequence Requirements ◽  
Better Than

ABSTRACT We performed two sets of in vitro selections to dissect the role of the −10 base sequence in determining the rate and efficiency with which Escherichia coli RNA polymerase-ς70forms stable complexes with a promoter. We identified sequences that (i) rapidly form heparin-resistant complexes with RNA polymerase or (ii) form heparin-resistant complexes at very low RNA polymerase concentrations. The sequences selected under the two conditions differ from each other and from the consensus −10 sequence. The selected promoters have the expected enhanced binding and kinetic properties and are functionally better than the consensus promoter sequence in directing RNA synthesis in vitro. Detailed analysis of the selected promoter functions shows that each step in this multistep pathway may have different sequence requirements, meaning that the sequence of a strong promoter does not contain the optimal sequence for each step but instead is a compromise sequence that allows all steps to proceed with minimal constraint.

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