In Vitro Localization of Bone Growth Factors in Constructs of Biodegradable Scaffolds Seeded with Marrow Stromal Cells and Cultured in a Flow Perfusion Bioreactor

SummaryThe osteogenic potential of red bone marrow was first reported more than 100 years ago. Since then, studies have reported controversial results that do not confirm nor disprove the capacity of fresh red bone marrow to produce bone. Researches have been focused on techniques that improve the efficiency of the bone marrow, including: the increase of the concentration of the mesenchymal stem cells in the aspirated bone marrow, the combination with a ‘carrier’ that helps to maintain the mesenchymal stem cells and guides and supports the vascular ingrowth in the defect, or the combination with bone growth factors that stimulate the marrow stromal cells to differentiate into bone forming cells. Each of these techniques has its drawbacks and increases the expenses of an operation. On the other hand, the synergistic effect observed with these combinations does not resolve the problem of the osteogenic capacity of pure bone marrow, which still remains questionable.

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Three-dimensional cell seeding and growth in radial-flow perfusion bioreactor for in vitro tissue reconstruction

Biotechnology and Bioengineering ◽

10.1002/bit.20797 ◽

2006 ◽

Vol 93 (5) ◽

pp. 947-954 ◽

Cited By ~ 47

Author(s):

Tatsuya Kitagawa ◽

Tetsuji Yamaoka ◽

Reiko Iwase ◽

Akira Murakami

Keyword(s):

Radial Flow ◽

Three Dimensional ◽

Perfusion Bioreactor ◽

Cell Seeding ◽

Flow Perfusion ◽

Tissue Reconstruction ◽

Flow Perfusion Bioreactor ◽

Dimensional Cell

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A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/873816 ◽

2009 ◽

Vol 2009 ◽

pp. 1-7 ◽

Cited By ~ 35

Author(s):

Alexander M. Sailon ◽

Alexander C. Allori ◽

Edward H. Davidson ◽

Derek D. Reformat ◽

Robert J. Allen ◽

...

Keyword(s):

Cell Culture ◽

Cell Density ◽

Three Dimensional ◽

Perfusion Bioreactor ◽

Peripheral Cell ◽

Cell Culture Techniques ◽

Culture Techniques ◽

Flow Perfusion ◽

Flow Perfusion Bioreactor

Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds.Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core.Results. By day 8, static scaffolds had a periphery cell density of , while in the core it was . Flow-perfused scaffolds demonstrated peripheral cell density of and core density of at day 8.Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems.

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