In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

2012 ◽  
Author(s):  
Chen-Wei Wu ◽  
Shilpa Sivashankar ◽  
Srinivasu Valagerahally Puttaswamy ◽  
Hui-Ling Lin ◽  
Kuo-Wei Chang ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Transgenic Mice ◽  
Liver Tissue ◽  
Hydrodynamic Flow ◽  
Perfusion Bioreactor ◽  
Flow Perfusion ◽  
Mice Liver ◽  
Flow Perfusion Bioreactor

scholarly journals A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Alexander M. Sailon ◽  
Alexander C. Allori ◽  
Edward H. Davidson ◽  
Derek D. Reformat ◽  
Robert J. Allen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Density ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Peripheral Cell ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds.Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core.Results. By day 8, static scaffolds had a periphery cell density of , while in the core it was . Flow-perfused scaffolds demonstrated peripheral cell density of and core density of at day 8.Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems.

Novel dual-flow perfusion bioreactor for in vitro pre-screening of nanoparticles delivery: design, characterization and testing

2021 ◽  
Author(s):  
Maria Elena Lombardo ◽  
Francesco Carfì Pavia ◽  
Emanuela Fabiola Craparo ◽  
Elisa Capuana ◽  
Gennara Cavallaro ◽  
...  
Keyword(s):  
Perfusion Bioreactor ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

In Vitro Liver Tissue Model Established from Transgenic Mice: Role of HIF-1alpha on Hypoxic Gene Expression

2006 ◽  
Vol 12 (11) ◽  
pp. 3135-3147 ◽  
Author(s):  
Jared W. Allen ◽  
Salman R. Khetani ◽  
Randall S. Johnson ◽  
Sangeeta N. Bhatia
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Liver Tissue ◽  
Tissue Model

Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)

2013 ◽  
Vol 176 ◽  
pp. 1081-1089 ◽  
Author(s):  
Shilpa Sivashankar ◽  
Srinivasu Valegerahally Puttaswamy ◽  
Ling-Hui Lin ◽  
Tz-Shuian Dai ◽  
Chau-Ting Yeh ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Ethylene Glycol ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Tissue Slices ◽  
Glycol Diacrylate ◽  
Poly Ethylene ◽  
Mice Liver
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