Luciferase Labeling for Multipotent Stromal Cell Tracking in Spinal Fusion Versus Ectopic Bone Tissue Engineering in Mice and Rats

2010 ◽  
Vol 16 (11) ◽  
pp. 3343-3351 ◽  
Author(s):  
Ruth E. Geuze ◽  
Henk-Jan Prins ◽  
F. Cumhur Öner ◽  
Yvonne J.M. van der Helm ◽  
Leontine S. Schuijff ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Spinal Fusion ◽  
Bone Tissue ◽  
Stromal Cell ◽  
Cell Tracking ◽  
Multipotent Stromal Cell ◽  
Ectopic Bone

scholarly journals Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

2016 ◽  
Vol 30 (6) ◽  
pp. 1122-1131 ◽  
Author(s):  
Jelena G. Najdanović ◽  
Vladimir J. Cvetković ◽  
Sanja Stojanović ◽  
Marija Đ. Vukelić-Nikolić ◽  
Maja M. Čakić-Milošević ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Histological Evaluation ◽  
Ectopic Bone

Bone tissue engineering and spinal fusion: the potential of hybrid constructs by combining osteoprogenitor cells and scaffolds

Biomaterials ◽  
2004 ◽  
Vol 25 (9) ◽  
pp. 1463-1473 ◽  
Author(s):  
M.C. Kruyt ◽  
S.M. van Gaalen ◽  
F.C. Oner ◽  
A.J. Verbout ◽  
J.D. de Bruijn ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Spinal Fusion ◽  
Bone Tissue ◽  
Osteoprogenitor Cells

scholarly journals Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142199546
Author(s):  
Lufei Wang ◽  
Dong Joon Lee ◽  
Han Han ◽  
Lixing Zhao ◽  
Hiroshi Tsukamoto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Real Time ◽  
Bone Tissue ◽  
Cell Tracking ◽  
3D Culture ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
3D Culture System

Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET) principle to improve BLI cell tracking and generated the brightest bioluminescent signal known to date, which thus enables more sensitive real-time cell tracking at deep tissue level. In the present study, we brought BRET-based cell tracking strategy into the field of bone tissue engineering for the first time. We labeled rat mesenchymal stem cells (rMSCs) with our in-house BRET-based GpNLuc reporter and evaluated the cell tracking efficacy both in vitro and in vivo. In scaffold-free spheroid 3D culture system, using BRET-based GpNLuc labeling resulted in significantly better correlation to cell numbers than a fluorescence based approach. In scaffold-based 3D culture system, GpNLuc-rMSCs displayed robust bioluminescence signals with minimal background noise. Furthermore, a tight correlation between BLI signal and cell number highlighted the robust reliability of using BRET-based BLI. In calvarial critical sized defect model, robust signal and the consistency in cell survival evaluation collectively supported BRET-based GpNLuc labeling as a reliable approach for non-invasively tracking MSC. In summary, BRET-based GpNLuc labeling is a robust, reliable, and inexpensive real-time cell tracking method, which offers a promising direction for the technological innovation of BLI and even non-invasive tracking systems, in the field of bone tissue engineering.

scholarly journals NIR fluorescence for monitoring in vivo scaffold degradation along with stem cell tracking in bone tissue engineering

Biomaterials ◽  
2020 ◽  
Vol 258 ◽  
pp. 120267 ◽  
Author(s):  
Soon Hee Kim ◽  
Ji Hoon Park ◽  
Jin Seon Kwon ◽  
Jae Gu Cho ◽  
Kate G. Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Tracking ◽  
Stem Cell Tracking ◽  
Nir Fluorescence ◽  
Scaffold Degradation

Labelling of Adipose Derived Mesenchymal Stem Cells for Cell Tracking Using MRI in Bone Tissue Engineering

2011 ◽  
Vol 55 (5) ◽  
pp. 369-377
Author(s):  
M. López-Laguna ◽  
A. Saldaña-Díaz ◽  
L. Rafael Ramos-Pascua ◽  
J.A. Rodríguez-Altónaga-Martínez ◽  
J. Ramos-Carro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Tracking

Bone tissue engineering via application of a collagen/hydroxyapatite 4D-printed biomimetic scaffold for spinal fusion

2021 ◽  
Vol 8 (2) ◽  
pp. 021403
Author(s):  
Hanjun Hwangbo ◽  
Hyeongjin Lee ◽  
Eun Ji Roh ◽  
WonJin Kim ◽  
Hari Prasad Joshi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Spinal Fusion ◽  
Bone Tissue ◽  
Biomimetic Scaffold
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