scholarly journals Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

2014 ◽  
pp. 140123085146001 ◽  
Author(s):  
Karl David Brown ◽  
Suet Low ◽  
Indumathi Mariappan ◽  
Keren Maree Abberton ◽  
Robert Short ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Contact Lenses ◽  
Limbal Stem Cell Deficiency ◽  
Plasma Polymer ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

scholarly journals Proteasome and Autophagy Pathway in Corneal Epithelial Cells with Limbal Stem Cell Deficiency

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Bardag-Gorce Fawzia ◽  
Lorenzo Michael Di ◽  
Niihara Robert ◽  
Sunada Sarah ◽  
Laporte Amanda ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Limbal Stem Cell Deficiency ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Autophagy Pathway ◽  
Limbal Stem Cell

Clinical Outcomes of Modified Simple Limbal Epithelial Transplantation for Limbal Stem Cell Deficiency in Chinese Population: A Retrospective Case Series

2021 ◽  
Author(s):  
Yinghui Wang ◽  
Xiaodan Hu ◽  
Ke Yang ◽  
Yang Zhang ◽  
Shijing Deng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Clinical Outcomes ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Successful Outcome ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

Abstract Objective To report clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD). Methods 13 patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalisation, vascularisation, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively. In 2 cases, in vivo confocal microscopy (IVCM) and impression cytology (IC) were performed to assess the proliferation and degeneration of limbal tissue. Results At a postoperative follow-up of 6.5 ± 5.3 (range, 2–20) months, 10 (10/13, 76.92%) eyes maintained a successful outcome. The grades of symblepharon, corneal conjunctivalisation, vascularisation, and opacification were significantly improved after SLET (P < 0.05). Two-line improvement in VA was found in 6 (6/10, 60%) eyes of the successful cases. Recurrence of LSCD occurred in 3 (3/13, 23.08%) eyes and conjunctival cyst occurred in 1 patient. After SLET, morphology and structure of corneal epithelial cells and epithelial transition around the limbal tissue fragments were detected by IVCM and IC. Conclusions Our findings suggest that the SLET is a safe and effective technique for the treatment of LSCD. The corneal stroma and hAM can provide protection and nutrition for the limbal stem cells (LSCs) without negatively influencing clinical outcomes. IVCM and IC after SLET can evaluate the effectiveness of surgery and the transition of LSCs and corneal epithelial cells.

scholarly journals Efficacy of Modified Amnion-Assisted Conjunctival Epithelial Redirection (ACER) for Partial Limbal Stem Cell Deficiency

Medicina ◽  
2021 ◽  
Vol 57 (4) ◽  
pp. 369
Author(s):  
Sang Beom Han ◽  
Farah Nur Ilyana Mohd Ibrahim ◽  
Yu-Chi Liu ◽  
Jodhbir S. Mehta
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cells ◽  
Corneal Surface ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell ◽  
Conjunctival Epithelial Cells

Background and objectives: the aim of this study was to analyze the efficacy of a modified “amnion-assisted conjunctival epithelial redirection (ACER)” technique for the treatment of partial limbal stem cell deficiency (LSCD). Materials and methods: the medical records of three patients with partial LSCD who underwent corneal surface reconstruction with modified ACER following superficial keratectomy were retrospectively studied. Briefly, in this technique, an inner amniotic membrane (AM) layer was applied on the corneal surface to promote corneal re-epithelialization. The outer AM layer was applied as a barrier to prevent the invasion of conjunctival epithelial cells into the cornea before the corneal surface was completely covered by corneal epithelial cells derived from the remaining intact limbal stem cells. Results: in all three cases, the outer AM layer successfully kept the conjunctival epithelium away from the corneal surface and prevented an admixture of conjunctival epithelial cells with corneal epithelial cells. In all three patients, the cornea was completely re-epithelized with epithelial cells derived from the remaining healthy limbal stem cells, and a clear visual axis was maintained without recurrence for a mean follow-up period of 37.3 ± 8.6 months. Conclusions: the preliminary results suggest that modified ACER appears to be a viable option for patients with partial LSCD.

scholarly journals Clinical outcomes of modified simple limbal epithelial transplantation for limbal stem cell deficiency in Chinese population: a retrospective case series

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yinghui Wang ◽  
Xiaodan Hu ◽  
Ke Yang ◽  
Yang Zhang ◽  
Shijing Deng ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Clinical Outcomes ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Successful Outcome ◽  
Limbal Stem Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

Abstract Objective To report the clinical outcomes of a novel surgical technique, namely simple limbal epithelial transplantation (SLET), for the treatment of limbal stem cell deficiency (LSCD). Methods Thirteen patients (13 eyes) with LSCD who underwent autologous (10 eyes) or allogeneic (3 eyes) modified SLET between 2018 and 2021 were enrolled in this study. Grades of symblepharon, corneal conjunctivalization, vascularization, opacification, and visual acuity (VA) were evaluated preoperatively and postoperatively. In 2 cases, in vivo confocal microscopy (IVCM) and impression cytology (IC) were performed to assess the proliferation and degeneration of limbal tissue. Results At a postoperative follow-up of 6.5±5.3 (range, 2–20) months, 10 (10/13, 76.92%) eyes maintained a successful outcome. The grades of symblepharon, corneal conjunctivalization, vascularization, and opacification were significantly improved after SLET (P<0.05). Two-line improvement in VA was found in 6 (6/10, 60%) eyes of the successful cases. Recurrence of LSCD occurred in 3 (3/13, 23.08%) eyes, and conjunctival cyst occurred in 1 patient. After SLET, the morphology and structure of corneal epithelial cells and epithelial transition around the limbal tissue fragments were detected by IVCM and IC. Conclusions Our findings suggest that the SLET is a safe and effective technique for the treatment of LSCD. The corneal stroma and hAM can provide protection and nutrition for the limbal stem cells (LSCs) without negatively influencing the clinical outcomes. IVCM and IC after SLET can evaluate the effectiveness of surgery and the transition of LSCs and corneal epithelial cells.

scholarly journals Subconjunctival Injection of Transdifferentiated Oral Mucosal Epithelial Cells for Limbal Stem Cell Deficiency in Rats

2020 ◽  
pp. 002215542098007
Author(s):  
Yu-Ting Xiao ◽  
Hua-Tao Xie ◽  
Xin Liu ◽  
Chao-Ye Duan ◽  
Jing-Yu Qu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Pigment Epithelium ◽  
Limbal Stem Cell Deficiency ◽  
Subconjunctival Injection ◽  
Epithelial Defect ◽  
Corneal Epithelial ◽  
Corneal Epithelial Defect ◽  
Limbal Stem Cell

Rat limbal niche cells (LNCs) have been proven to induce transdifferentiation of oral mucosal epithelial cells (OMECs) into corneal epithelial-like cells termed transdifferentiated oral mucosal epithelial cells (T-OMECs). This investigation aimed to evaluate the effect of subconjunctival T-OMEC injections on alkali-induced limbal stem cell deficiency (LSCD) in rats. LNCs were cocultured with OMECs in the Transwell system to obtain T-OMECs, with NIH-3T3 cells serving as a control. Subconjunctival injection of single T-OMEC or OMEC suspension was performed immediately after corneal alkali injury. T-OMECs were prelabeled with the fluorescent dye CM-DiI in vitro and tracked in vivo. Corneal epithelial defect, opacity, and neovascularization were quantitatively analyzed. The degree of corneal epithelial defect (from day 1 onward), opacity (from day 5 onward), and neovascularization (from day 2 onward) was significantly less in the T-OMEC group than in the OMEC group. Cytokeratin 12 (CK12), pigment epithelium–derived factor, and soluble fms-like tyrosine kinase-1 were expressed at a higher rate following T-OMEC injection. Some CM-DiI-labeled cells were found to be coexpressed with CK12, Pax6, and ΔNp63α in the corneal epithelium after subconjunctival injection. Subconjunctival injection of T-OMECs prevents conjunctival invasion and maintains a normal corneal phenotype, which might be a novel strategy in the treatment of LSCD:

scholarly journals Two distinct populations of corneal epithelial cells with limbal stem cell characteristics in the mouse

2008 ◽  
Vol 86 ◽  
pp. 0-0
Author(s):  
V HOLAN ◽  
M KRULOVA ◽  
K POKORNA ◽  
A LENCOVA ◽  
A ZAJICOVA ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Limbal Stem Cell

scholarly journals A Review of Limbal Stem Cell Deficiency

2019 ◽  
Vol 2 (1) ◽  
pp. e4-e11
Author(s):  
John E. Conto
Keyword(s):  
Stem Cell ◽  
Contact Lenses ◽  
Systemic Disease ◽  
Anterior Segment ◽  
Treatment Strategies ◽  
Limbal Stem Cell Deficiency ◽  
Contact Lens Wear ◽  
Limbal Stem Cell Transplantation ◽  
Limbal Stem Cell ◽  
Amniotic Membranes

Limbal stem cell deficiency (LSCD) can be secondary to multiple etiologies including contact lens wear, chemical or thermal trauma, and systemic disease, any of which can result in the reduction of the number of stem cells or their decreased functionality. Primary LSCD is seen with a variety of congenital anterior segment disorders. Often LSCD can be stabilized and timely diagnosis is the key. The use of topical corticosteroids and artificial tear lubricants, along with treatment of any underlying conditions, and discontinuation of contact lenses are important initial treatment strategies. Advance treatments include amniotic membranes, limbal stem cell transplantation and keratoprostheses.

Sign in / Sign up

Export Citation Format

Share Document