Serosurvey of Crimean-Congo Hemorrhagic Fever Virus in Domestic Animals, Gujarat, India, 2013

2014 ◽  
Vol 14 (9) ◽  
pp. 690-692 ◽  
Author(s):  
Devendra T. Mourya ◽  
Pragya D. Yadav ◽  
Anita Shete ◽  
Triparna D. Majumdar ◽  
Amit Kanani ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Domestic Animals ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

scholarly journals The world areal of Crimean-Congo hemorrhagic fever virus

2006 ◽  
Vol 5 ◽  
pp. 79-86 ◽  
Author(s):  
S. Ye. Smirnova
Keyword(s):  
Hemorrhagic Fever ◽  
Domestic Animals ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Immunological Status ◽  
The World ◽  
Cchf Virus

Distribution of Crimean-Congo hemorrhagic fever (CCHF) virus based on virus isolations and results of seroepidemiological surveys. The were investigated 147 cases of ill (4 countries of Europe and Asia), immunological status of population (13 countries of Europe, Asia and Africa) and domestic animals (16 countries of Europe, Asia and Africa) and infected ticks (7 countries of Eu- rope and Asia). CCHF was first recognized in Azerbaijan, Armenia, Afghanistan, Iran, India and confirmed in Russia, Ukraine, Bulgaria, Yugoslavia (Europe), Uzbekistan, Tadzhikistan, Turkmenistan, Kazakhstan (Asia) and Uganda, Senegal, Kenya (Africa).

scholarly journals Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies

2019 ◽  
Vol 65 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Anne Rackow ◽  
Christa Ehmen ◽  
Ronald von Possel ◽  
Raquel Medialdea-Carrera ◽  
David Brown ◽  
...  
Keyword(s):  
Zika Virus ◽  
Specific Binding ◽  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Size Exclusion ◽  
Animal Origin ◽  
Serum Samples ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Igm Antibodies

Abstract BACKGROUND The cellular surface molecule HsTOSO/FAIM3/HsFcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain (HsFcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV). METHODS His-tagged HsFcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of HsFcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on HsFcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate. RESULTS Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with HsFcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of HsFcμR-Igl as a capture molecule in aggregation-based rapid tests. CONCLUSIONS Recombinant HsFcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests.

scholarly journals Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda

2021 ◽  
Vol 290 ◽  
pp. 114075
Author(s):  
Stephen Balinandi ◽  
Claudia von Brömssen ◽  
Alex Tumusiime ◽  
Jackson Kyondo ◽  
Hyesoo Kwon ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Molecular Study ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus

scholarly journals Persistence of Crimean-Congo Hemorrhagic Fever Virus RNA

2020 ◽  
Vol 26 (2) ◽  
pp. 385-387
Author(s):  
Leholonolo Mathengtheng ◽  
Dominique Goedhals ◽  
Phillip A. Bester ◽  
Jacqueline Goedhals ◽  
Felicity J. Burt
Keyword(s):  
Hemorrhagic Fever ◽  
Fever Virus ◽  
Crimean Congo Hemorrhagic Fever ◽  
Hemorrhagic Fever Virus ◽  
Virus Rna
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