scholarly journals Long-term consumption of isoflavone-enriched foods does not affect bone mineral density, bone metabolism, or hormonal status in early postmenopausal women: a randomized, double-blind, placebo controlled study

2008 ◽  
Vol 87 (3) ◽  
pp. 761-770 ◽  
Author(s):  
Elizabeth Brink ◽  
Veronique Coxam ◽  
Simon Robins ◽  
Kristiina Wahala ◽  
Aedin Cassidy ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Metabolism ◽  
Postmenopausal Women ◽  
Controlled Study ◽  
Hormonal Status ◽  
Mineral Density ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Early Postmenopausal Women

A simple enzyme-linked immunosorbent assay of human osteocalcin

1994 ◽  
Vol 40 (7) ◽  
pp. 1258-1264 ◽  
Author(s):  
C Rosenquist ◽  
M Bonde ◽  
C Fledelius ◽  
P Qvist
Keyword(s):  
Postmenopausal Women ◽  
Polyclonal Antibodies ◽  
Premenopausal Women ◽  
Controlled Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Osteocalcin ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Early Postmenopausal Women ◽  
Log Linear

Abstract A heterologous ELISA for measurement of osteocalcin (bone Gla-protein) is described, involving biotinylated bovine osteocalcin and polyclonal antibodies. The log-linear range was 2.3-37.5 micrograms/L. Between-run (total) and within-run CVs (n = 10) were 5.7-6.4 and 5.9-6.1%, respectively; analytical recoveries ranged from 92% to 108%. Comparison of our method (x) with an RIA (y) yielded y = 1.10 x -0.01 microgram/L, Sylx = 1.4 micrograms/L, r = 0.958 (n = 167). Serum osteocalcin in healthy premenopausal women (n = 29) was 8.7 +/- 3.3 micrograms/L (mean +/- SD) and 11.8 +/- 4.5 micrograms/L in early-postmenopausal women (n = 24). The assay was evaluated in a double-blind placebo-controlled study of healthy early-postmenopausal women, treated for 12 months with either (a) estrogen valerate plus medroxyprogesterone acetate (n = 18), (b) 17 beta-estradiol and desogestrel (n = 22), or (c) placebo (n = 17). Serum osteocalcin decreased significantly (P < 0.001) with either therapy, but increased (P < 0.05) with placebo.

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