scholarly journals Behavioral Responses of Newly Hatched Zebrafish (Danio rerio) to Amino Acid Chemostimulants

2004 ◽  
Vol 29 (2) ◽  
pp. 93-100 ◽  
Author(s):  
S. M. Lindsay
2016 ◽  
Vol 15 (10) ◽  
pp. 2171-2179 ◽  
Author(s):  
Jin-Song Zhang ◽  
Yi Huang ◽  
Xiao-Bo Han ◽  
Ting-Lin Huang ◽  
Alice K. Y. Chan

1973 ◽  
Vol 61 (1) ◽  
pp. 74-88 ◽  
Author(s):  
Nancy R. Goldrich

Behavioral responses to labellar stimulation with 19 L-amino acids were predicted on the basis of electrophysiological responses of largest labellar hairs. With the exceptions alanine, aspartic and glutamic acids, and valine tests of these predictions confirmed that Phormia can discriminate amino acids, and that these acids may be grouped according to their effects. Electrophysiological investigation of the four exceptions was repeated and results were consistent with the behavioral data. In particular, these acids elicited previously unreported responses from the salt receptor. The discrepancies between this and earlier studies may be explainable, in part, on methodological grounds. There was evidence for response differences among hairs of different sizes and among the largest labellar hairs themselves. The significance of amino acid discrimination for the problem of protein recognition can only be speculated upon until more complete electrophysiological and nutritional information is available.


2015 ◽  
Vol 10 (2) ◽  
Author(s):  
M. Murwantoko ◽  
Chio Oka ◽  
Masashi Kawaichi

HtrA which is characterized by the combination of a trypsin-like catalytic domain with at least one C-terminalPDZ domain is a highly conserved family of serine proteases found in a wide range of organisms. However theidentified HtrA family numbers varies among spesies, for example the number of mammalian, Eschericia coli,fruit fly-HtrA family are 4, 3 and 1 gene respectively. One gene is predicted exist in zebrafish. Since no completeinformation available on zebrafish HtrA, in this paper zebrafish HtrA (zHtrA) gene was analyzed. The zHtrA isbelonged to HtrA1 member and predicted encodes 478 amino acids with a signal peptide, a IGF binding domain,a Kazal-type inhibitor domain in the up stream of HtrA-bacterial homolog. At the amino acid sequence the zHtrA1showed the 69%, 69%, 68%, 54% and 54% with the rat HtrA1, mouse HtrA1, human HtrA1, human HtrA3 andmouse HtrA4 respectively. The zHtrA1 is firstly expressed at 60 hpf and mainly in the vertebral rudiments in thetail region.


2016 ◽  
Vol 148 ◽  
pp. 401-408 ◽  
Author(s):  
Siti Hawatulaila Baharuddin ◽  
Noorul Adawiyah Mustahil ◽  
Atikah Aini Abdullah ◽  
Magaret Sivapragasam ◽  
Muhammad Moniruzzaman
Keyword(s):  

Behaviour ◽  
2012 ◽  
Vol 149 (10-12) ◽  
pp. 1205-1240 ◽  
Author(s):  
Simone Dereje ◽  
Susanna Sawyer ◽  
Sarah E. Oxendine ◽  
Linran Zhou ◽  
Zachary D. Kezios ◽  
...  

2020 ◽  
Author(s):  
Qiang-Sheng Zhu ◽  
Jie Wang ◽  
shan he ◽  
Xu-Fang Liang ◽  
Shuang Xie ◽  
...  

Abstract Background Early nutritional programming affects a series of metabolism, growth and development in mammals. Fish also exhibit the developmental plasticity by early nutritional programming. However, little is known about the effect of early amino acid programming on growth and metabolism. Methods In the present study, zebrafish (Danio rerio) was used as the experimental animal to study whether early leucine stimulation can programmatically affect the mechanistic target of rapamycin (mTOR) signaling pathway, growth and metabolism in the later life, and to undercover the mechanism of epigenetic regulation. Zebrafish larvas at 3 days post hatching (dph) were raised with 1.0% leucine from 3 to 13 dph during the critical developmental stage, then back to normal water for 70 days (83 dph). Results The growth performance and crude protein content of zebrafish in the early leucine programming group were increased, and consistent with the activation of the mTOR signaling pathway and the high expression of genes involved in the metabolism of amino acid and glycolipid. Furthermore, we compared the DNA methylation profiles between the control and leucine-stimulated zebrafish, and found that the methylation levels of CG-differentially methylated regions (DMGs) and CHH-DMGs of genes involved in mTOR signaling pathway were different between the two groups. With quantitative PCR analysis, the decreased methylation levels of CG type of Growth factor receptor-bound protein 10 (Grb10), eukaryotic translation initiation factor 4E (eIF4E) and mTOR genes of mTOR signaling pathway in the leucine programming group, might contribute to the enhanced gene expression. Conclusions The early leucine programming could improve the protein synthesis and growth, which might be attributed to the methylation of genes in mTOR pathway and the expression of genes involved in protein synthesis and glycolipid metabolism in zebrafish. These results could be beneficial for better understanding of the epigenetic regulatory mechanism of early nutritional programming.


2020 ◽  
Author(s):  
Qiang-Sheng Zhu ◽  
Jie Wang ◽  
shan he ◽  
Xu-Fang Liang ◽  
Shuang Xie ◽  
...  

Abstract Background: Early nutritional programming affects a series of metabolism, growth and development in mammals. Fish also exhibit the developmental plasticity by early nutritional programming. However, little is known about the effect of early amino acid programming on growth and metabolism. Methods: In the present study, zebrafish (Danio rerio) was used as the experimental animal to study whether early leucine stimulation can programmatically affect the mechanistic target of rapamycin (mTOR) signaling pathway, growth and metabolism in the later life, and to undercover the mechanism of epigenetic regulation. Zebrafish larvas at 3 days post hatching (dph) were raised with 1.0% leucine from 3 to 13 dph during the critical developmental stage, then back to normal water for 70 days (83 dph). Results: The growth performance and crude protein content of zebrafish in the early leucine programming group were increased, and consistent with the activation of the mTOR signaling pathway and the high expression of genes involved in the metabolism of amino acid and glycolipid. Furthermore, we compared the DNA methylation profiles between the control and leucine-stimulated zebrafish, and found that the methylation levels of CG-differentially methylated regions (DMGs) and CHH-DMGs of genes involved in mTOR signaling pathway were different between the two groups. With quantitative PCR analysis, the decreased methylation levels of CG type of Growth factor receptor-bound protein 10 (Grb10), eukaryotic translation initiation factor 4E (eIF4E) and mTOR genes of mTOR signaling pathway in the leucine programming group, might contribute to the enhanced gene expression. Conclusions: The early leucine programming could improve the protein synthesis and growth, which might be attributed to the methylation of genes in mTOR pathway and the expression of genes involved in protein synthesis and glycolipid metabolism in zebrafish. These results could be beneficial for better understanding of the epigenetic regulatory mechanism of early nutritional programming.


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