A Method for Quantifying Small Amounts of Glucose or Glycerol in Agar Culture Media. Gas-Liquid Chromatography of Trimethylsilyl Derivatives With Reduced Pyridine Tailing

1972 ◽  
Vol 10 (10) ◽  
pp. 639-641 ◽  
Author(s):  
F. Calhoun ◽  
H. B. Howe
Keyword(s):  
Liquid Chromatography ◽  
Culture Media ◽  
Gas Liquid Chromatography ◽  
Agar Culture ◽  
Trimethylsilyl Derivatives

Mannitol estimation in biological fluids by gas-liquid chromatography of trimethylsilyl derivatives.

1980 ◽  
Vol 26 (3) ◽  
pp. 441-443 ◽  
Author(s):  
M F Laker ◽  
J N Mount
Keyword(s):  
Liquid Chromatography ◽  
Biological Fluids ◽  
Internal Standard ◽  
Peak Height ◽  
Gas Liquid Chromatography ◽  
Height Measurement ◽  
Standard Solution ◽  
Trimethylsilyl Derivatives

Abstract We describe a procedure for estimating mannitol concentrations in biological fluids. Samples are mixed with internal standard solution (alpha-methylglucose), deproteinized if necessary, desalted, and dried. Specimens are then derivatized by adding pyridine/bis(trimethylsilyl)acetamide/trimethylchlorosilane and heating at 60 degrees C for 30 min. Samples are chromatographed on a 275-cm column of 10% OV-17, operated at 190 degrees C, and quantitated by peak-height measurement. The technique is linear, accurate, precise, sensitive, and free from interference. It has been used to measure mannitol in plasma, urine, and bile.

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