Simplified Manual Micromethod for Determination of Serum Triglycerides

Abstract This rapid, simplified assay for serum triglycerides is based on the use of a more effective solvent extraction procedure, ethoxide transesterification, and color development with acetylacetone. No phospholipid adsorption step is necessary. The procedure requires less than 0.2 ml of serum, no expensive or elaborate apparatus, and 5 to 10 values can be obtained per hour, with better precision than with most existing methods. Recovery averaged 99.9%. There are practically no interferences. Results correlated excellently with those obtained by the classical Carlson-Wadström method.

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Simple Manual Procedure for Determination of Serum Triglycerides

Clinical Chemistry ◽

10.1093/clinchem/21.6.768 ◽

1975 ◽

Vol 21 (6) ◽

pp. 768-770 ◽

Cited By ~ 29

Author(s):

Jose Mendez ◽

Barry Franklin ◽

Harry Gahagan

Keyword(s):

Quality Control ◽

Calibration Curve ◽

Room Temperature ◽

Extraction Procedure ◽

Serum Triglycerides ◽

Modified Method ◽

Color Development ◽

Significant Difference ◽

Control Samples

Abstract We describe a modified method for determining serum triglycerides (triacylglycerols), which is based on the heptane extraction procedure of Gottfried and Rosenberg [Clin. Chem. 19, 1077 (1973)] with the stable saponification, oxidation, and color development reagents of Neri and Frings [Clin. Chem. 19, 1201 (1973)]. This modified method eliminates one heating step, reduces saponification time to 5 min, absorbances are read at room temperature, and the calibration curve is linear to 3.0 g/liter. A sample comparison between the proposed method and the automated Block and Jarrett [Am. J. Med. Technol. 35, 1 (1969)] procedure showed no significant difference (r = 0.98). The coefficient of variation (47 duplicate samples) for the modified method was 6.3%. Further validation was obtained from analysis of quality-control samples; the proposed method gave equivalent values.

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Examination of Conditions for the Determination of Carbamate Pesticides in Soils

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19921632 ◽

1992 ◽

Vol 57 (8) ◽

pp. 1632-1638 ◽

Cited By ~ 1

Author(s):

Věra Tatarkovičová ◽

Zdeněk Stránský

Keyword(s):

Activated Carbon ◽

Solvent Extraction ◽

Soil Type ◽

Extraction Procedure ◽

Purification Procedure ◽

Factors Affecting ◽

Carbamate Pesticides ◽

Rp Hplc ◽

Extracting Solvent

The procedure for the determination of carbamate pesticides in soil was optimized. The following factors affecting the final results were investigated: extracting solvent, extraction procedure, extract purification procedure, and soil type. Triple extraction with acetone and purification of the extract on a two-stage purification column containing an activated carbon-silica gel 1+1 mixture were found optimal. The extracts after treatment were analyzed by RP-HPLC with UV detection. The method developed allows carbamate pesticides in soil to be determined at concentrations in excess of 30 μg kg-1.

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Gas-Liquid Chromatographic Determination of Dioxathion and Quintiofos in Organophosphorus Dip Washes

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/63.1.33 ◽

1980 ◽

Vol 63 (1) ◽

pp. 33-36

Author(s):

Molly I Keating

Keyword(s):

Solvent Extraction ◽

Extraction Procedure ◽

Internal Standard ◽

Chromatographic Determination ◽

Phosphorus Compounds ◽

Ionization Detector ◽

Liquid Chromatographic Determination ◽

Gasliquid Chromatography ◽

Liquid Chromatographic

Abstract A rapid method for the analysis of dip washes is described which eliminates the usual solvent extraction procedure. The dip wash is initially diluted with acetone and then with petroleum ether. The diluted dip wash is analyzed by gasliquid chromatography, using an alkali ionization detector sensitive to phosphorus compounds. The method was applied to the determination of dioxathion (2,3-ρ-dioxanedithiol S,S-bis(O,O-diethyl phosphorodithioate)), and quintiofos (O-ethyl O-8-quinolyl phenylphosphonothioate) dip washes. Average recoveries from fouled dip washes were 100 and 104%. GLC of these compounds with an internal standard is described, which improves the precision of the method to ±2%.

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