Biological Applications of the Carbon Rod Atomizer in Atomic Absorption Spectroscopy. 2. Determination of Copper in Small Samples of Tissue

1972 ◽  
Vol 18 (11) ◽  
pp. 1379-1384 ◽  
Author(s):  
Brian J Stevens
Keyword(s):  
Neutron Activation Analysis ◽  
Atomic Absorption ◽  
Copper Content ◽  
Simple Technique ◽  
Small Samples ◽  
Biological Applications ◽  
Flame Atomic Absorption ◽  
Determination Of Copper ◽  
Carbon Rod Atomizer

Abstract A simple technique is presented for the analysis for copper in small fragments of tissue. Dissolution of the tissue in concentrated nitric acid is the only preparative step required. Copper content is then determined by comparison with standards, by using the carbon rod atomizer previously described. Sample volumes of less than 2 µl are used for each measurement. The method gives results that compare well with both colorimetric and flame atomic absorption methods, and also with neutron activation analysis, and appears to be applicable to the analysis of other metals in tissue.

Determination of copper in powdered chocolate samples by slurry-sampling flame atomic-absorption spectrometry

2005 ◽  
Vol 382 (4) ◽  
pp. 1099-1102 ◽  
Author(s):  
Walter N. L. dos Santos ◽  
Erik G. P. da Silva ◽  
Marcelo S. Fernandes ◽  
Rennan G. O. Araujo ◽  
Antônio C. S. Costa ◽  
...  
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flame Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Slurry Sampling ◽  
Flame Atomic Absorption ◽  
Determination Of Copper

Automated determination of copper in undiluted serum by atomic absorption spectroscopy.

1981 ◽  
Vol 27 (8) ◽  
pp. 1438-1440 ◽  
Author(s):  
N Weinstock ◽  
M Uhlemann
Keyword(s):  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Capillary Tube ◽  
Sample Volume ◽  
Dilution Method ◽  
Flame Atomic Absorption ◽  
Undiluted Serum ◽  
Determination Of Copper

Abstract An injection method has been adapted for the determination of copper concentration in untreated, undiluted serum by flame atomic absorption spectroscopy. Serum, 50 or 100 microliter, is automatically injected by a commercial microprobe system into a plastic cone connected to the capillary tube of the burner, at a rate of 240 samples per hour. The required sample volume is considerably decreased, and sensitivity is increased 20- to 40-fold. After 500 measurements we observed no memory effects, carryover, or clogging of the burner. We discuss common difficulties with calibration standards due to viscosity and other physicochemical interferences, and suggest the use of pooled human serum as a secondary standard. Within-run CV was 1.8%, the day-to-day CV 2.2%. Comparison with a dilution method gave a correlation coefficient exceeding 0.98.

A Simple and Rapid Dispersive Liquid–Liquid Microextraction Based on Solidification of Floating Organic Drop Method Combined with Flame Atomic Absorption Spectrometry for Preconcentration and Determination of Copper

2013 ◽  
Vol 96 (2) ◽  
pp. 441-446 ◽  
Author(s):  
Mohammad Mirzaei ◽  
Mansoureh Behzadi
Keyword(s):  
Complex Formation ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flame Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Flame Atomic Absorption ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Determination Of Copper

Abstract A simple and rapid dispersive liquid–liquid microextraction based on solidification of floating organic drop method prior to flame atomic absorption spectrometry was developed for preconcentration and determination of copper. In this technique, simultaneous complex formation and extraction was performed with rapid injection of a mixture containing ethanol, 1-undecanol, and 1-(2-pyridylazo)-2-naphthol into a water sample spiked with Cu(II). After centrifugation, the test tube was cooled in an ice bath, and solidified extract transferred into a conical vial. Finally, it was dissolved into ethanol and copper concentration was determined. Some effective parameters of extraction and complex formation, such as extraction and disperser solvent type and volume, pH, concentration of the chelating agent, salt effect, and extraction time, were optimized. Under the optimum conditions, the calibration graph was linear in the range of 0.50 ng/mL to 0.30 μg/mL, with an LOD of 0.16 ng/mL. The RSD for 10 replicate measurements of 50.0 ng/mL of copper was ±1.4%. Two certified reference materials were analyzed, and the determined values were in good agreement with the certified values.

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