Quantitative Gas-Chromatographic Determination of Short-Chain Fatty Acids in Aqueous Samples
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Abstract We report the use of SP-1200 (Supelco Inc.) for quantitative gas-chromatographic determination of short-chain fatty acids (C2-C5) in stool water. The ratio of peak areas for these acids to that for 2-methylvaleric acid (internal standard) is linear for each acid from 60 to 1800 mg/liter. Lactic acid, occasionally present in stool in abnormally high amounts, is not detectable as the free acid on SP-1200, but is determined as its propyl ester on diethyleneglycolsuccinate.
2000 ◽
Vol 50
(4)
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pp. 231-236
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2009 ◽
Vol 32
(7)
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pp. 1027-1035
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2010 ◽
Vol 1217
(22)
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pp. 3561-3566
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2019 ◽
Vol 42
(5)
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pp. 845-849
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