Automated Method for Determination of Bound N-Acetylneuraminic Acid in Serum

1975 ◽  
Vol 21 (3) ◽  
pp. 412-414 ◽  
Author(s):  
Elisabeth Rey ◽  
Lucile Gerbaut ◽  
Christian Lombart
Keyword(s):  
Sialic Acid ◽  
Present Method ◽  
Acetylneuraminic Acid ◽  
Automated Method ◽  
Resorcinol Method ◽  
Acid Determination

Abstract A modified resorcinol method for sialic acid determination has been successfully adapted to the Technicon AutoAnalyzer. The present method requires only 25 µl of serum, and 30 samples can be analyzed per hour. It may be used to measure sialic acid in concentrations ranging from 10 to 100 mg/liter.

Improved Automated Determination of Bound N-Acetylneuraminic Acid in Serum

1973 ◽  
Vol 19 (11) ◽  
pp. 1285-1287 ◽  
Author(s):  
Lucile Gerbaut ◽  
Elisabeth Rey ◽  
Christian Lombart
Keyword(s):  
Sialic Acid ◽  
Organic Solvent ◽  
Thiobarbituric Acid ◽  
Acetylneuraminic Acid ◽  
Automated Determination ◽  
Entire Procedure ◽  
Acid Determination

Abstract The method in which thiobarbituric acid is used for sialic acid determination was adapted to a fully automated procedure, in which the chromogen need not be extracted with an organic solvent. The entire procedure, including hydrolysis, requires 35 min and 25 µl of serum for one analysis, but 30 samples can be analyzed per hour. The procedure, slightly modified, can be used to measure as little as 10 mg of bound sialic acid per liter.

A Modified Semi-automated Resorcinol Method for the Determination of Inulin

1969 ◽  
Vol 15 (11) ◽  
pp. 1072-1078 ◽  
Author(s):  
Thomas H Steele
Keyword(s):  
Present Method ◽  
Protein Precipitation ◽  
Accuracy And Precision ◽  
Precipitation Procedure ◽  
High Degree ◽  
Automated Methods ◽  
Resorcinol Method ◽  
Urine Specimens

Abstract A semi-automated resorcinol method for the determination of inulin in plasma and urine specimens, using standard AutoAnalyzer modules, is described. Plasma and other protein-containing fluids undergo a preliminary manual protein precipitation procedure. The deproteinized specimens are processed at a rate of 50/hr, with a high degree of accuracy and precision. The superiority of the present method is largely due to the elimination of small acid-resistant pump tubes from the manifold. This is possible because reagents are premixed prior to aspiration into the system, allowing simplification of manifold design over other automated methods.

Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135, and BO

1976 ◽  
Vol 54 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Apurba K. Bhattacharjee ◽  
Harold J. Jennings ◽  
C. Paul Kenny ◽  
Adèle Martin ◽  
Ian C. P. Smith
Keyword(s):  
Sialic Acid ◽  
Acetyl Derivative ◽  
Major Product ◽  
Hydroxyl Group ◽  
Nmr Studies ◽  
Acetylneuraminic Acid ◽  
Nmr Data ◽  
Polysaccharide Antigens ◽  
C Nmr

The purified high molecular weight serogoup Y meningococcal polysaccharide contains equimolar proportions of D-glucose and N-acetylneuraminic acid and is partially O-acetylated. Carbon-13 nuclear magnetic resonance (NMR) studies, together with other chemical data, have indicated that the polysaccharide is linked only at C-6 of the D-glucose and C-4 of the sialic acid residues, all the linkages being in the α-configuration. The 13C NMR data also indicated that the Y polysaccharide is composed of an alternating sequence of these two different residues, and this was confirmed by its autohydrolysis where the major product was 4-O-α-D-glucopyranosyl-β-D-N-acetylneuraminic acid. The W-135 polysaccharide differs from that of Y only in the absence of O-acetylation and in the configuration of one hydroxyl group of the disaccharide repeating unit. In this case autohydrolysis yielded 4-O-α-D-galactopyranosyl-β-D-N-acetylneuraminic acid as the major product. Structural evidence indicates that the BO and Y polysaccharides are identical.Methanolysis of the Y polysaccharide yielded in addition to the methyl glycosides of glucose and sialic acid, a 9-O-acetyl derivative of the latter. This derivative was formed during the re-N-acetylation process and its formation was mainly due to the presence of sodium ions in the original polysaccharide.

Evaluation of 1,10-Phenanthroline as a Reagent for Sialic Acid Determinations

1974 ◽  
Vol 20 (3) ◽  
pp. 387-388 ◽  
Author(s):  
S L Snyder ◽  
N S Mathewson ◽  
P Z Sobocinski
Keyword(s):  
Sialic Acid ◽  
Complex Formation ◽  
Sialic Acids ◽  
Previous Investigator ◽  
Acetylneuraminic Acid ◽  
Specific Reagent

Abstract Use of o-phenanthroline as a reagent for determination of sialic acids was proposed by a previous investigator. This method was based on an increase in absorbance at 307 nm that occurred when solutions of o-phenanthroline and various sialic acids were mixed. It was postulated that the increased absorbance was a result of formation of specific complexes. Using N-acetylneuraminic acid, we found no evidence for complex formation. Our results indicate that the observations of the previous investigator resulted from shifts in the pH of the medium rather than from formation of specific complexes. Therefore o-phenanthroline is not a specific reagent for sialic acids and its use is not recommended.

Usefulness and Potential Pitfalls of Sialic Acid Determination in Sera of Patients with Ovarian Tumors

1986 ◽  
Vol 1 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Bruno Berra ◽  
Silvana Rapelli ◽  
Gianluigi Monticelli ◽  
Maria Albertina Fighetti ◽  
Ines Della Mea ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Tumor Marker ◽  
Cancer Patients ◽  
Preliminary Data ◽  
Ovarian Tumors ◽  
Serum Sialic Acid ◽  
Methodological Errors ◽  
Acid Determination

Increasing evidence in the literature indicates that serum sialic acid is increased in cancer patients suggesting a possible usefulness of its determination as a tumor marker. However there are many discrepancies in the data reported, probably due to methodological errors, mainly in lipid bound sialic measurement. In this paper we illustrate the results obtained when we applied a method worked out in our laboratory for the determination of total and fractionated sialic acid (lipid and protein bound) to the analysis of sera from patients with ovarian tumors and the preliminary data on the follow up of selected cases. The potential pitfalls in using this relatively new tumor marker will be critically evaluated.

Glucose suppression of deoxyribose interference in the thiobarbituric acid determination of sialic acid

1975 ◽  
Vol 67 (1) ◽  
pp. 290-297 ◽  
Author(s):  
Carl H. Smith ◽  
Thomas M. Donohue ◽  
Rosalyn Depper
Keyword(s):  
Sialic Acid ◽  
Thiobarbituric Acid ◽  
Acid Determination

An improved automated periodate-resorcinol method for the determination of sialic acid

1983 ◽  
Vol 133 (1) ◽  
pp. 233-238 ◽  
Author(s):  
Theodore R. Oegema ◽  
Katherine M. Cooper
Keyword(s):  
Sialic Acid ◽  
Resorcinol Method

Determination of Plasminogen in Human Plasma by the Lysis Time Method

1966 ◽  
Vol 16 (01/02) ◽  
pp. 001-017 ◽  
Author(s):  
W Berg ◽  
K Korsan-Bengtsen ◽  
J Ygge
Keyword(s):  
Human Plasma ◽  
Present Method ◽  
Blood Donors ◽  
Fibrinogen Concentration ◽  
Plasmin Activity ◽  
Time System ◽  
Lysis Time ◽  
High Dilutions ◽  
Single Determination

SummaryA one-stage lysis time system containing fibrinogen, streptokinase, thrombin, and a known, small amount of plasminogen was used to determine plasminogen in plasma.The known amount of plasminogen was added to the system in order to keep the lysis times relatively short when a highly diluted plasma was tested. High dilutions of plasma were used to reduce the influence of the plasma inhibitors.The calculation of the plasminogen concentration was made on the basis of the correlation: “plasminogen = fibrinogen/lysis time” which was valid in the system. The method allowed determination of plasminogen in plasma with varying fibrinogen concentrations, as the fibrinogen concentration in plasma was considered in the calculation.The presence of “spontaneous” plasmin activity in the plasma did not influence the plasminogen determination. Estimated by this method, the plasminogen content in plasma from 32 blood donors aged 25-45 years was 13.1 ±2.4 casein u/ml. The error of a single determination was 0.3 casein u/ml. The plasminogen content in plasma, determined with the present method, is about 3-4 times higher than the content found when a caseinolytic method is used.

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