A Modified Semi-automated Resorcinol Method for the Determination of Inulin

1969 ◽  
Vol 15 (11) ◽  
pp. 1072-1078 ◽  
Author(s):  
Thomas H Steele
Keyword(s):  
Present Method ◽  
Protein Precipitation ◽  
Accuracy And Precision ◽  
Precipitation Procedure ◽  
High Degree ◽  
Automated Methods ◽  
Resorcinol Method ◽  
Urine Specimens

Abstract A semi-automated resorcinol method for the determination of inulin in plasma and urine specimens, using standard AutoAnalyzer modules, is described. Plasma and other protein-containing fluids undergo a preliminary manual protein precipitation procedure. The deproteinized specimens are processed at a rate of 50/hr, with a high degree of accuracy and precision. The superiority of the present method is largely due to the elimination of small acid-resistant pump tubes from the manifold. This is possible because reagents are premixed prior to aspiration into the system, allowing simplification of manifold design over other automated methods.

Micromethod for determination of creatinine in biological fluids by high-performance liquid chromatography.

1978 ◽  
Vol 24 (5) ◽  
pp. 747-750 ◽  
Author(s):  
S J Soldin ◽  
J G Hill
Keyword(s):  
High Performance Liquid Chromatography ◽  
Continuous Flow ◽  
Present Method ◽  
High Performance ◽  
Biological Fluids ◽  
Coefficients Of Variation ◽  
Analytical Recovery ◽  
Urine Specimens ◽  
Serum And Urine

Abstract We describe a procedure for the rapid and specific measurement of creatinine, in which it is separated from other compounds in serum or urine by paired-ion chromatography and is quantified by measuring its absorbance at 200 nm. The procedure can be done on as little as 10 microliter of serum. Between-day precision studies for concentrations of 13 and 62 mg/liter yielded coefficients of variation of 6.9 and 2.2%, respectively. Analytical recovery of various amounts of creatinine added to plasma exceeded 95% in all cases. The proposed procedure was compared with the continuous-flow procedure by analyzing a series of serum and urine specimens by both methods. There was excellent agreement for urine specimens, but with serum the results by the present method were significantly (P less than 0.001) lower.

scholarly journals The simultaneous spectrophotometric determination of trimazolin and phenylephrine hydrochloride in nasal preparations

2008 ◽  
Vol 14 (4) ◽  
pp. 261-264 ◽  
Author(s):  
Ivana Savic ◽  
Goran Nikolic ◽  
Ivan Savic ◽  
Vladimir Bankovic
Keyword(s):  
Spectrophotometric Determination ◽  
Pharmaceutical Formulations ◽  
Active Components ◽  
Phenylephrine Hydrochloride ◽  
Drug Substances ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Relative Standard ◽  
High Degree

This paper presents the experimental results for the simultaneous spectrophotometric determination of two active components in nasal solutions. The resolution of two-component mixtures of timazolin and phenylephrine has been accomplished by using partial least-squares. The method comprised of the absorptivity measurement in a nasal solution at wavelengths of 265 and 272 nm, respectively. Notwithstanding the presence of two components and their high degree of spectral overlap, they have been determined simultaneously with high accuracy and precision, with no interference, rapidly and without resorting to extraction procedures using non aqueous solvents. This method was tested and validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation <2 %), while being simple, cheap and less time consuming. The method was applied for the analysis of these drug substances in their commercial pharmaceutical formulations.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF RAMOSETRON HYDROCHLORIDE IN BULK AND TABLETS

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (07) ◽  
pp. 54-57
Author(s):  
P. Sathyanarayana ◽  
◽  
M Vijayalakshmi ◽  
B. N. V Ravi Kumar
Keyword(s):  
Flow Rate ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Rp Hplc ◽  
Accuracy And Precision ◽  
Development And Validation ◽  
High Degree ◽  
Isocratic Mode

A RP-HPLC method was developed and validated for the determination of ramosetron hydrochloride in pharmaceutical formulation as per ICH and FDA guidelines. The method was carried out on a Phenomenex RP-C18 column using a mixture of methanol and water (95:5) in an isocratic mode. The flow rate is 0.8 mL/min and the detection was done at 302 nm. The linearity range was observed in the range of 1-6 mcg/mL. The accuracy of the method was found to be 99.0 to 99.5% and %RSD was found to be less than 2% indicating high degree of accuracy and precision for the proposed HPLC method. Limit of detection and limit of quantification of the method were found to be 0.028 and 0.0851 mcg/mL respectively.

Determination of Copolymer Composition by Combustion Analysis for Carbon and Hydrogen

1960 ◽  
Vol 33 (4) ◽  
pp. 1132-1141
Author(s):  
Lawrence A. Wood ◽  
Irving Madorsky ◽  
Rolf A. Paulson
Keyword(s):  
Refractive Index ◽  
Present Method ◽  
Copolymer Composition ◽  
Combustion Analysis ◽  
Physical Methods ◽  
Accuracy And Precision ◽  
Styrene Content ◽  
The One ◽  
Relative Measurements

Abstract The procedure described attains its accuracy and precision by the refinement and improvement of conventional simple operations over a periods of years. The trend in analysis recently is, of course, toward the use of rapid physical methods. In a great many instances these involve relative measurements requiring the initial establishment of reference materials with compositions determined by methods such as the one here described, which bases the numbers derived solely on readings of an analytical balance. In fact, as already mentioned, one of the principal applications of the present method has been in the establishment of the relation between refractive index and styrene content for SBR polymers so that refractive index measurements can be used in routine determinations of bound styrene content.

The Ultramicro Determination of Salicylates in Biologic Fluids

1962 ◽  
Vol 8 (4) ◽  
pp. 400-404 ◽  
Author(s):  
Albert Hanok
Keyword(s):  
Spinal Fluid ◽  
Pediatric Practice ◽  
Color Development ◽  
Accuracy And Precision ◽  
Normal Individuals ◽  
Salicylate Poisoning ◽  
Poisoning In Children ◽  
High Degree

Abstract A method for determining salicylates in ultramicro volumes of blood, spinal fluid, and urine has been investigated. This method combines the simplicity of one reagent for color development and deproteinization with a high degree of accuracy and precision. Apparent salicylate values in blood and spinal fluid of normal individuals are of the order of 0.7 mg./100 ml. The application of this method to analysis of capillary samples of blood would be of value in the treatment of salicylate poisoning in children and in the maintenance of therapeutic salicylate levels in pediatric practice.

THE POLAROGRAPHIC DETERMINATION OF TIN IN HIGH PURITY ZINC AND ZINC DIE-CASTING ALLOYS

1947 ◽  
Vol 25b (3) ◽  
pp. 322-330 ◽  
Author(s):  
R. C. Hawkings ◽  
D. Simpson ◽  
H. G. Thode
Keyword(s):  
Hydrogen Peroxide ◽  
Sulphuric Acid ◽  
High Purity ◽  
Die Casting ◽  
Polarographic Determination ◽  
Casting Alloys ◽  
Accuracy And Precision ◽  
High Degree ◽  
Copper Lead

A procedure has been developed for the determination of tin in high-purity zinc and zinc die-casting alloys present in amounts from 0.001 to 0.2%. The samples are dissolved in sulphuric acid, oxidized with hydrogen peroxide, precipitated with cupferron, redissolved and reduced, and finally the tin is determined polarographically. By this method, the tin can be determined with an accuracy of ± 2.0% when present in amounts less than 0.005%. A method is now available for the routine polarographic determination of trace amounts of copper, lead, cadmium, and tin in high-purity zinc and zinc die-casting alloys with a high degree of accuracy and precision.

Automated Method for Determination of Bound N-Acetylneuraminic Acid in Serum

1975 ◽  
Vol 21 (3) ◽  
pp. 412-414 ◽  
Author(s):  
Elisabeth Rey ◽  
Lucile Gerbaut ◽  
Christian Lombart
Keyword(s):  
Sialic Acid ◽  
Present Method ◽  
Acetylneuraminic Acid ◽  
Automated Method ◽  
Resorcinol Method ◽  
Acid Determination

Abstract A modified resorcinol method for sialic acid determination has been successfully adapted to the Technicon AutoAnalyzer. The present method requires only 25 µl of serum, and 30 samples can be analyzed per hour. It may be used to measure sialic acid in concentrations ranging from 10 to 100 mg/liter.

Simplified, totally enzymatic method for determination of serum triglycerides with a centrifugal analyzer.

1976 ◽  
Vol 22 (8) ◽  
pp. 1310-1313 ◽  
Author(s):  
S H Grossman ◽  
E Mollo ◽  
G Ertingshausen
Keyword(s):  
Present Method ◽  
Room Temperature ◽  
Fixed Time ◽  
Glycerol Dehydrogenase ◽  
Enzymatic Method ◽  
Serum Triglycerides ◽  
Acceptable Accuracy ◽  
Accuracy And Precision ◽  
Enzymatic Procedure

Abstract We describe a totally enzymatic method for determination of serum triglycerides (triacylglycerols) specifically adaptable to the CentrifiChem system. The method involves lipolysis with lipase from Rhizopus arrhizus alone and quantitation of the resulting glycerol with glycerol dehydrogenase in a kinetic, fixed-time mode. Hydrolysis by the lipase is complete, for concentrations up to at least 5.0 g/liter, in 10 min at room temperature. The unfavorable equilibrium for the oxidation of glycerol is overcome by increasing the pH and adding excess NAD+. Under these conditions the glycerol determination is linear to at least 4.0 g of glycerol per liter, as triglyceride. The test exhibits acceptable accuracy and precision, and results correlate well with those by an alternative totally enzymatic procedure. The present method is unaffected by phosphatase and a considerably simplified reagent is used.

Feasibility of Molecular Structure Determination With a High Resolution Electron Microscope

1968 ◽  
Vol 26 ◽  
pp. 338-339
Author(s):  
T. A. Welton
Keyword(s):  
Electron Microscope ◽  
Substrate Surface ◽  
Helical Axis ◽  
Base Plane ◽  
Resolution Electron ◽  
High Resolution Electron Microscope ◽  
High Degree ◽  
Degree Of Order ◽  
Purine Pyrimidine

An ultimate design goal for an improved electron microscope, aimed at biological applications, is the determination of the structure of complex bio-molecules. As a prototype of this class of problems, we propose to examine the possibility of reading DNA sequence by an imaginable instrument design. This problem ideally combines absolute importance and relative simplicity, in as much as the problem of enzyme structure seems to be a much more difficult one.The proposed technique involves the deposition on a thin graphite lamina of intact double helical DNA rods. If the structure can be maintained under vacuum conditions, we can then make use of the high degree of order to greatly reduce the work involved in discriminating between the four possible purine-pyrimidine arrangements in each base plane. The phosphorus atoms of the back bone form in projection (the helical axis being necessarily parallel to the substrate surface) two intertwined sinusoids. If these phosphorus atoms have been located up to a certain point on the molecule, we have available excellent information on the orientation of the base plane at that point, and can then locate in projection the key atoms for discrimination of the four alternatives.

Determination of Plasminogen in Human Plasma by the Lysis Time Method

1966 ◽  
Vol 16 (01/02) ◽  
pp. 001-017 ◽  
Author(s):  
W Berg ◽  
K Korsan-Bengtsen ◽  
J Ygge
Keyword(s):  
Human Plasma ◽  
Present Method ◽  
Blood Donors ◽  
Fibrinogen Concentration ◽  
Plasmin Activity ◽  
Time System ◽  
Lysis Time ◽  
High Dilutions ◽  
Single Determination

SummaryA one-stage lysis time system containing fibrinogen, streptokinase, thrombin, and a known, small amount of plasminogen was used to determine plasminogen in plasma.The known amount of plasminogen was added to the system in order to keep the lysis times relatively short when a highly diluted plasma was tested. High dilutions of plasma were used to reduce the influence of the plasma inhibitors.The calculation of the plasminogen concentration was made on the basis of the correlation: “plasminogen = fibrinogen/lysis time” which was valid in the system. The method allowed determination of plasminogen in plasma with varying fibrinogen concentrations, as the fibrinogen concentration in plasma was considered in the calculation.The presence of “spontaneous” plasmin activity in the plasma did not influence the plasminogen determination. Estimated by this method, the plasminogen content in plasma from 32 blood donors aged 25-45 years was 13.1 ±2.4 casein u/ml. The error of a single determination was 0.3 casein u/ml. The plasminogen content in plasma, determined with the present method, is about 3-4 times higher than the content found when a caseinolytic method is used.

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