Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes.

1982 ◽  
Vol 28 (2) ◽  
pp. 311-316 ◽  
Author(s):  
P A Pleban ◽  
A Munyani ◽  
J Beachum
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Zeeman Effect ◽  
Selenium Concentration ◽  
Glutathione Peroxidase Activity ◽  
Plasma Selenium ◽  
Plasma Glutathione Peroxidase ◽  
Flameless Atomic Absorption Spectroscopy ◽  
Plasma Glutathione

Abstract We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC 1.11.1.9), in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a mean erythrocyte selenium concentration of 149.9 (SD 8.3) microgram/L (n = 10) and 6.4% for a mean plasma selenium concentration of 97.3 (SD 6.2) microgram/L (n = 12). For the women, mean selenium concentrations were 141.4 (SD 14.3) microgram/L of erythrocytes [0.49 (SD 0.07) microgram/g of hemoglobin and 96.3 (SD 14.2) microgram/L of plasma. Glutathione peroxidase activities were measured by a modification of the method of Paglia and Valentine (J. Lab. Clin. Med. 70: 158--169, 1967). Within-run precision studies for the glutathione peroxidase assays gave CVs of 12.8% for mean erythrocyte glutathione peroxidase activity of 77.2 (SD 9.9) U/g of hemoglobin (n = 13), and 8.1% for mean plasma activity of 312.5 (SD 25.2) U/L (n = 11). Mean enzyme activity was 78.7 (SD 12.9) U/g of hemoglobin for erythrocytes and 424 (SD 40) U/L for plasma. Erythrocyte selenium concentrations and glutathione peroxidase activities were positively, but poorly, correlated (r = 0.41, p less than 0.01).

Plasma Glutathione Peroxidase Activity in Preeclampsia

1988 ◽  
Vol 75 (s19) ◽  
pp. 43P-43P
Author(s):  
R.M. Berry ◽  
N.A. Punchard ◽  
N.A. MacLachlan ◽  
R.P.H. Thompson
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
Plasma Glutathione Peroxidase ◽  
Plasma Glutathione

Comparative effects of selenium supplementation on the plasma selenium concentration and erythrocyte glutathione peroxidase activity in cattle and camels

1998 ◽  
Vol 67 (3) ◽  
pp. 461-466 ◽  
Author(s):  
M. Bengoumi ◽  
A. K. Essamadi ◽  
J. C. Tressol ◽  
J. P. Chacornac ◽  
B. Faye
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Selenium Concentration ◽  
Basal Diet ◽  
Glutathione Peroxidase Activity ◽  
Plasma Selenium ◽  
Selenium Metabolism ◽  
Mineral Supplementation ◽  
Very High ◽  
Plasma Selenium Concentration

AbstractAt an experimental farm, five camels and five cows were each given a similar basal diet for 6 months. They received oral trace element supplementation for 3 months (day 22 to 112) which included zinc, copper, selenium, manganese, iodine and cobalt and corresponded to twice the daily requirement generally recommended for cows. Plasma selenium concentrations were significantly lower in the camels (20 (s.e. 2) mg/l) compared with the cows (33 (s.e. 2) μg/l). The mineral supplementation induced a large increase in the plasma selenium concentration in the camels which reached 200 (s.e. 35) fig/l. In the cows, the increase was much smaller and did not go beyond 65 (s.e. 8) μg/l. Before supplementation the red blood cell glutathione peroxidase activity was similar in the camel and the cow varying between 4000 and 6000 IU per 100 g haemoglobin. In both species, this activity increased with mineral supplementation and remained very high even when mineral supplementation was stopped. The results suggested that selenium metabolism in camels is different from that in the cows.

scholarly journals Correlation Between Age, Body Mass Index, And Blood Selenium Level with Glutathione Peroxidase Activity Among Elderly in South Jakarta

2020 ◽  
Vol 4 (2) ◽  
pp. 89
Author(s):  
Annisa Nurul Kirana ◽  
Erfi Prafiantini ◽  
Novi Silvia Hardiany
Keyword(s):  
Body Mass Index ◽  
Glutathione Peroxidase ◽  
Body Mass ◽  
Peroxidase Activity ◽  
Selenium Level ◽  
Glutathione Peroxidase Activity ◽  
Cross Sectional ◽  
Plasma Glutathione Peroxidase ◽  
Plasma Glutathione ◽  
Nonparametric Correlation

Oxidative stress contributed in aging process and several degenerative diseases. Selenium was an important trace element due to as a component of antioxidants enzymes (selenoproteins), including glutathione peroxidase for protection against free radical.Objective: We aimed to study the correlation between blood selenium level and plasma glutathione peroxidase activity in elderly.Materials and Methods: Cross sectional study was held in 5 elderly communities in south Jakarta. Body mass index, blood selenium level and plasma glutathione peroxidase activity were measured in 95 elderly aged between 60-86 years old. Nonparametric correlation was used for correlation analysis.Results and Discussion: The median of subject’s age was 69 years old (60-86) and for body mass index was 23.57 (13.59-36.05). The median of selenium level among subject was 0.19 (0.023-0.56). The mean of plasma glutathione peroxidase activity was 164.45 U/L ± 68.07. There was no correlation among variables. However, plasma glutathione peroxidase activity decreased with increasing age and body mass index although it was not significant.Conclusion: There was no correlation between blood selenium level and plasma glutathione peroxidase activity. Detection of plasma selenium level is needed to confirm this result.International Journal of Human and Health Sciences Vol. 04 No. 02 April’20 Page : 89-93

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