Consumer Awareness and Perception on Mineral and Vitamin Supplements to Boost Immunity Against COVID-19

COVID-19 have been widespread all over the world. Mineral supplementation and Vitamin intake may help to reduce the severity of the common cold. This study determines the Knowledge, Attitude, And Practices of Consumers About Mineral and Vitamin Supplementation to boost immune system for COVID-19. 178 Participants who lived in the NCR Bubble particularly Metro Manila, Laguna, Cavite, Rizal, and Bulacan., 18-years old and above were chosen by convenience sampling. To get the appropriate data needed, the researchers divided the questionnaire into 4 parts such as Demographics, Knowledge, Attitude, and Practices using likert scale. Descriptive analysis of frequencies and percentages for demographic characteristics, KAP assessment and likewert interpretation was applied. The respondents' knowledge, attitude, and practices toward micronutrient supplementation were evaluated. Results showed that the respondents have a very good understanding, belief, and practice of mineral supplementation and vitamin intake to boost the immune system. Before the pandemic, 178 respondents did not take mineral supplements; nevertheless, 58 respondents (32.58 percent) took vitamins, and 117 respondents (65.73%) took both mineral and multivitamin supplements. On the other hand, 61 (34.27%) respondents took vitamins, while 120 (67.42%) respondents took both mineral and vitamin supplements during the pandemic. In addition, the intake of these supplements increased during pandemic. Self-willingness (28.65%) was the most common response to factors linked with mineral and vitamin consumption prior to the pandemic, whereas doctor's prescription was the most common response during the pandemic (44.94%). The level of micronutrient supplementation practiced by the respondents was also applied because they knew and believed that it could reduce the risk of COVID-19 infection.

A Research-Based Summary on Trace Minerals for Cattle

Producers can utilize mineral supplementation to improve cattle production and herd health, which has the potential to reduce antibiotic usage. This publication presents a summary on trace minerals for cattle. Written by João H. Jabur Bittar and Roberto A. Palomares, and published by the UF/IFAS Veterinary Medicine—Large Animal Clinical Sciences Department, October 2021.

Effects of Sex Class, a Combined Androgen and Estrogen Implant, and Pasture Supplementation on Growth and Carcass Performance and Meat Quality of Zebu-Type Grass-Fed Cattle

Forty-seven Zebu calves were used to determine the effects of class (bull or steer), supplementation (SUPPL, a poultry litter-based supplement or mineral supplementation), and implant (20 mg estradiol combined with 120 mg of trenbolone acetate or no implant) on growth and carcass performance and beef eating quality. The average daily gain (ADG) of implanted cattle significantly increased for steers, but not for bulls. The SUPPL treatment increased ADG by 8.63% from day 0 to end, and shortened in 73.3 d the time to reach 480 kg BW (p < 0.01). Compared to bulls, the steer carcasses exhibited more desirable maturity and finish scores, thicker back fat (p < 0.05), and yielded greater (p < 0.01) percentages of high-value boneless subprimals (HVBLS) (+1.64%) and total cuts (1.35%). The SUPPL bulls dressed 2.63 and 1.63% greater than non-supplemented bulls and SUPPL steers, respectively (p < 0.05). Meat sensory quality was subtly affected (p < 0.05) by sex class or supplementation. The implant did not affect (p > 0.05) shear force or sensory ratings. The supplementation improved key growth performance traits while it adversely affected tenderness-related sensory traits. The implant enhanced the rate of gain of steers only, without improving cut-out yields or inducing adverse effects on palatability traits in both steers and bulls.

PSIII-25 The effects of varying levels of trace mineral supplementation on mineral balance and antibody concentrations in feedlot cattle

Trace mineral (TM) supplementation is critical for optimal performance and health. The objective was to determine the effects of varying levels of TM supplementation on feedlot cattle TM status and antibody concentrations. Angus steers (n = 240; BW = 291 kg ± 27.4) were stratified by arrival BW and source and assigned to 1 of 4 treatments (12 pens/treatment; 5 steers/pen). Treatments included a negative control (CON) in which cattle received no TM supplementation, a requirement treatment (1X) in which cattle received added TM at 2016 Nutrient Requirements of Beef Cattle required levels, a 2 times requirements (2X), and a 4 times requirements (4X) treatment. Selenium was included at 0.1, 0.2, and 0.3 mg/kg for 1X, 2X, and 4X respectively. Treatments were chosen based on reported TM supplementation levels from the 2015 Feedlot Consulting Nutritionist Survey. One steer was chosen at random from each pen to be evaluated for serum and liver TM status and antibody concentrations to respiratory viruses. There was treatment × day interaction for serum Co, and liver Cu and Se (P &lt; 0.0001). Serum Co was greatest for the 4X treatment from d 28 through harvest. Liver Cu was greatest for the 2X and 4X treatments from d 56 through harvest. Liver Se was greatest for 2X and 4X from d 28 through harvest. There was an effect of day on liver Co, Fe, Mn, Mo, and Zn (P &lt; 0.0001) and serum Cu, Mn, Mo, Se, and Zn (P &lt; 0.0019). Concentrations for individual TM had different trends over time, however, all reported values were within normal ranges. Serum Zn was greater at harvest (P = .02). There was an effect of time on Bovine Viral Diarrhea Virus Type 1A, Bovine Herpesvirus Type 1, Bovine Parainfluenza 3 virus antibody titer concentrations (P &lt; 0.0001).

254 Effects of Nutrition on Bull Fertility

The greatest effects of nutrition on bull fertility occur by providing a relatively high plane of nutrition during calfhood when the seminiferous epithelium of the testes is developing. A high plane of nutrition from 5 to 25 weeks of age results in greater circulating gonadotropin concentrations, greater testicular volume, and greater sperm production at maturity. At this age, diet can be manipulated by creep feeding bull calves with both protein and energy supplements which could accelerate puberty and increase sperm production. Increased rate of gain in bull calves post-weaning can accelerate puberty a few weeks, but excess post-weaning gain and fat buildup in the neck of the scrotum can have detrimental effects on fertility. Considerable emphasis has been placed on specific micronutrient (especially minerals) effects on peripubertal bull fertility. Trace mineral supplementation is believed to be critical for optimal fertility and both copper (Cu) and zinc (Zn) supplementation have influenced male fertility in other species. However, there are no guidelines for recommended levels of these minerals to ensure fertility. Providing organic or complexed compared to inorganic mineral to peripubertal bulls have increased liver concentrations of mineral and in some cases, have accelerated puberty, but enhanced fertility-associated measures have not been realized. Because both Cu and Zn are present in the ejaculate, mineral needs during the breeding season may differ from the off-season. During the breeding season, bulls can lose 10 to 20% of their weight and need to re-gain this weight before the next breeding season. Divergent planes of nutrition provided to mature bulls during the off-season revealed enhanced sperm energy and stress-fighting potential with decreasing plane of nutrition. Specific mineral supplementation during the off-season in mature bulls provides no beneficial effect on fertility-associated measures. In summary, significant effects of nutrition post-weaning on bull fertility-associated measures have not been realized.

PSV-1 Injectable trace mineral supplementation on grazing beef cows: Effect on subsequent offspring birth weight and weaning weight

This study evaluated the effect of an injectable multi-mineral complex supplementation on grazing beef cows on subsequent offspring birth weight (BW) and adjusted weaning weight (AWW). Experiment 1 included 1,128 Angus crossbred multiparous suckled cows, from 9 locations in Virginia, that were randomly assigned to one of two treatments: 1) One dose of an injectable trace mineral (6 mL; Multimin® 90, Multimin USA, Ft. Collins, CO) at 71.4±16.8 d of lactation (n = 560); or 2) a negative control with no trace mineral injection (n = 568). In experiment 2, 986 Angus crossbred multiparous suckled cows, at 7 locations in Virginia, were randomly assigned to one of two treatments: 1) Two doses of an injectable trace mineral (6 mL; Multimin® 90) at 118.3±17 days before calving and at 67.7±17.4 d of lactation (n = 494); or 2) negative control with no trace mineral injection (n = 492). All cows had ad libitum access to forage and water, and received additional mineral supplementation in form of mineral blocks. Calves were weaned at approximately 7 months of age, and 205-day AWW was calculated based on birth day, age of weaning and age of dam. No differences between treatments were detected for BW (33.7±1.01 kg; P = 0.50) and AWW (212.2±5.0 kg; P = 0.25) when only one dose of injectable trace mineral was administered to the dam at early lactation. Similarly, when two doses of injectable trace mineral were administrated BW (35.6±1.1 kg; P = 0.70) and AWW (259.4±6.1 kg; P = 0.83) did not differ between treatments. In conclusion, one or two doses of injectable trace mineral administrated at late gestation and/or during lactation had no effect on offspring BW and AWW.

PSXIII-3 Effects of mineral source and additive in mineral supplementation on productive performance of growing beef cattle on tropical pastures

Our objective was to evaluate the effects of mineral supplementation using the association of carboaminophosphochelates as mineral source plus Salinomycin on grazing beef cattle performance during growing phase. A total of 80 Nellore bulls (IBW = 292.72 ± 13.55 kg; Age, ~13 mo) were distributed in 8 paddocks (~2.7 hectares each) planted with Palisade Grass cv. Xaraés during the rainy to rainy-to-dry transition seasons (February to May). The experiment was a randomized complete block design (10 animals/paddock, 4 paddocks per treatment). The mineral supplementation was provided ad libitum and treatments were: Control; Mineral supplementation containing inorganic minerals, and TM+Salinomycin = Mineral supplementation containing carboaminophosphochelates (TM, Tortuga® Minerals) as minerals source (Cr, Se, Zn, Mn, S, Cu e Co) plus Salinomycin (~108 mg per animal/day). The supplement orts were removed and weighted daily to calculate supplement intake. The animals were weighed after a 14 h-fasting at the beginnig and end of the trial (84 days) to calculate weight gain. The paddock was considered the experimental unit and data were analyzed using PROC MIXED of SAS, whereby means were separated using the PDIFF statement (P &lt; 0.05). Supplement intake was similar between treatments (P = 0.13). Animals supplemented with TM+Salinomycin tended to have greater final BW (358 vs. 347, P = 0.07) and increased ADG in 22,3% (0.730 vs. 0.597 kg/d, P = 0.03) compared with animals fed Control. In conclusion, the combination of carboaminophosphochelates minerals plus salynomicin improves grazing beef cattle weight gain, with no deleterious effect on mineral supplement intake.

302 Vitamin and Mineral Supplementation and Rate of Gain in Beef Heifers: Effects on Fetal Trace Mineral Reserves at Day 83 of Gestation

Thirty-five crossbred Angus heifers (body weight = 359.5 &gt;± 7.1 kg) were randomly assigned to a 2 × 2 factorial design to evaluate the effects of vitamin and mineral supplementation [VMSUP; supplemented (VTM) vs. unsupplemented (NoVTM)] and rate of gain [GAIN; low gain (LG), 0.28 kg/d vs. moderate gain (MG), 0.79 kg/d] during the first 83 d of gestation on trace mineral concentrations in fetal liver, muscle, and allantoic (ALF) and amniotic (AMF) fluids. The VTM treatment (113 g supplement•heifer-1•d-1) was initiated a minimum 71 d before breeding. At breeding, heifers were either maintained on the basal diet (LG) or received the MG diet by adding a protein/energy supplement to the basal diet. On d 83 of gestation, samples of fetal liver, muscle, ALF, and AMF were collected and analyzed for trace mineral concentrations. In fetal liver, Se, Cu, Mn, and Co concentrations were greater (P ≤ 0.04) for VTM than NoVTM, while Mo and Co greater (P ≤ 0.04) for LG than MG. In fetal muscle, VTM increased (P ≤ 0.02) concentrations of Se and Zn, whereas LG increased (P &lt; 0.01) Zn. In ALF, Mo concentrations were affected (P = 0.03) by a VMSUP × GAIN interaction, with VTM-MG greater than NoVTM-MG; while VTM increased (P &lt; 0.01) concentrations of Se and Co. Trace mineral concentrations were not affected (P ≥ 0.13) in AMF. In conclusion, VTM increased fetal liver Se, Cu, Mn, and Co concentrations; fetal muscle Se and Zn; and ALF Se and Co; while LG increased fetal liver Mo and Co concentrations and fetal muscle Zn. Our results confirm that managerial decisions associated with vitamin and mineral supplementation and rate of gain can alter fetal reserves of trace elements during early pregnancy.

PSVIII-22 Maternal vitamin and mineral supplementation affect fetal hepatic expression of genes underlying mineral homeostasis and lipid metabolism in early pregnancy

Vitamins and minerals play critical roles in functions such as hormone production, DNA synthesis, regulation of gene expression, and lipid metabolism. However, the impact of vitamin and mineral supplementation on fetal programming and the interplay with gene expression of fetal organs remains unclear. We used a differential gene expression analysis to determine effects of maternal vitamin and mineral supplementation (from pre-breeding to d 83 post-breeding) on fetal hepatic gene expression and the pathways underlying liver function and metabolism at 83 d of gestation. Crossbred Angus beef heifers were supplemented (VTM, n = 7) or not (CON, n = 8) with 113 g•heifer-1•d-1 of mineral premix (Purina® Wind & Rain Storm All-Season 7.5 Complete) from a minimum d 71 before breeding through d 83 of gestation. After breeding, heifers were fed to gain 0.79 kg/d. All heifers were surgically ovariohysterectomized on d 83 and fetal liver collected. Total RNA was isolated from the fetal liver (n = 15) and gene expression measured with RNA-Seq. After library quality control and read mapping, differential expression was performed using edgeR. We identified 53 genes upregulated and 37 downregulated in the VTM group (adj.Pval &lt; 0.1). Genes involved with mineral homeostasis, such as MT1A, MT1E, and MT2A, were among those differentially expressed underlying the mineral absorption pathway. ABCA1 and ABCA6, which are involved in cholesterol and metal ion transport across the plasma membrane, and PPARG and SDR16C5, that act on lipoprotein transport and metabolism, were upregulated in the VTM group. Also upregulated in the VTM group, the CUBN gene plays a role in vitamin and iron metabolism. In summary, maternal vitamin and mineral supplementation from pre-breeding to d 83 of gestation leads to upregulation of fetal hepatic genes acting on mineral homeostasis, lipid transport, and metabolism.