Direct measurement of antigens in serum by time-resolved fluoroimmunoassay.

1985 ◽  
Vol 31 (1) ◽  
pp. 50-53 ◽  
Author(s):  
J E Kuo ◽  
K H Milby ◽  
W D Hinsberg ◽  
P R Poole ◽  
V L McGuffin ◽  
...  
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Immunoglobulin G ◽  
Chelating Agent ◽  
Competitive Binding ◽  
Fluorescence Immunoassay ◽  
Time Resolved ◽  
Rapid Procedure ◽  
Bifunctional Chelating Agent ◽  
Fluorescence Label

Abstract A long-lived fluorescence label (Tb3+) has been attached to the antigen of interest by using a bifunctional chelating agent 1-(p-benzenediazonium)-EDTA. A nonequilibrium competitive-binding immunoassay protocol, in conjunction with time-resolved detection of the long-lived fluorescence label, allows the antigen to be analyzed directly in samples containing diluted human serum. Results obtained for immunoglobulin G with this simple and rapid procedure correlated well (r = 0.93) with those by a commercially available fluorescence immunoassay method.

scholarly journals Development of a Time-Resolved Fluorescence Immunoassay for Epstein–Barr Virus Zta IgA Antibodies in Human Serum

2015 ◽  
Vol 28 (3) ◽  
pp. 179-183 ◽  
Author(s):  
Juanjuan Chen ◽  
Tiancai Liu ◽  
Zhenhua Chen ◽  
Jingyuan Hou ◽  
Yingsong Wu ◽  
...  
Keyword(s):  
Human Serum ◽  
Epstein Barr Virus ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Barr Virus ◽  
Iga Antibodies ◽  
Epstein Barr

scholarly journals The differences in short- and long-term varicella-zoster virus (VZV) immunoglobulin G levels following varicella vaccination of healthcare workers measured by VZV fluorescent-antibody-to-membrane-antigen assay (FAMA), VZV time-resolved fluorescence immunoassay and a VZV purified glycoprotein enzyme immunoassay

2016 ◽  
Vol 144 (11) ◽  
pp. 2345-2353 ◽  
Author(s):  
P. A. C. MAPLE ◽  
J. HAEDICKE ◽  
M. QUINLIVAN ◽  
S. P. STEINBERG ◽  
A. A. GERSHON ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Healthcare Workers ◽  
Fluorescent Antibody ◽  
Varicella Vaccination ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Varicella Zoster ◽  
Antigen Assay

SUMMARYHealthcare workers (HCWs) reporting no history of varicella frequently receive varicella vaccination (vOka) if they test varicella-zoster virus (VZV) immunoglobulin G (IgG) negative. In this study, the utilities of VZV-IgG time-resolved fluorescence immunoassay (VZV-TRFIA) and a commercial VZV-IgG purified glycoprotein enzyme immunoassay (gpEIA) currently used in England for confirming VZV immunity have been compared to the fluorescent-antibody-to-membrane-antigen assay (FAMA). A total of 110 HCWs received two doses of vOka vaccine spaced 6 weeks apart and sera collected pre-vaccination (n = 100), at 6 weeks post-completion of vaccination (n = 86) and at 12–18 months follow-up (n = 73) were analysed. Pre-vaccination, by FAMA, 61·0% sera were VZV IgG negative, and compared to FAMA the sensitivities of VZV-TRFIA and gpEIA were 74·4% [95% confidence interval (CI) 57·9–87·0] and 46·2% (95% CI 30·1–62·8), respectively. Post-completion of vaccination the seroconversion rate by FAMA was 93·7% compared to rates of 95·8% and 70·8% determined by VZV-TRFIA and gpEIA, respectively. At 12–18 months follow-up seropositivity rates by FAMA, VZV-TRFIA and gpEIA were 78·1%, 74·0% and 47·9%, respectively. Compared to FAMA the sensitivities of VZV-TRFIA and gpEIA for measuring VZV IgG following vaccination were 96·4% (95% CI 91·7–98·8) and 74·6% (95% CI 66·5–81·6), respectively. Using both FAMA and VZV-TRFIA to identify healthy adult VZV susceptibles and measure seroconversion showed that vOka vaccination of HCWs is highly immunogenic.

Development of a time-resolved fluorescence immunoassay for Epstein-Barr virus nuclear antigen 1-immunoglobulin A in human serum

2015 ◽  
Vol 87 (11) ◽  
pp. 1940-1945 ◽  
Author(s):  
Juan-Juan Chen ◽  
Tian-Cai Liu ◽  
Qian-Ni Liang ◽  
Zhi-Ning Dong ◽  
Ying-Song Wu ◽  
...  
Keyword(s):  
Human Serum ◽  
Epstein Barr Virus ◽  
Immunoglobulin A ◽  
Nuclear Antigen ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Performance of a Time-Resolved Fluorescence Immunoassay for Measuring Varicella-Zoster Virus Immunoglobulin G Levels in Adults and Comparison with Commercial Enzyme Immunoassays and Merck Glycoprotein Enzyme Immunoassay

2006 ◽  
Vol 13 (2) ◽  
pp. 214-218 ◽  
Author(s):  
P. A. C. Maple ◽  
J. Gray ◽  
J. Breuer ◽  
G. Kafatos ◽  
S. Parker ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Immunoglobulin G ◽  
High Specificity ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Commercial Enzyme ◽  
Varicella Zoster ◽  
Enzyme Immunoassays ◽  
Human Sera

ABSTRACT Highly sensitive and specific, quantitative assays are needed to detect varicella-zoster virus (VZV) immunoglobulin G in human sera, particularly for determining immune status and response following vaccination. A time-resolved fluorescence immunoassay (TRFIA) has been developed, and its performance was compared to that of two commercial enzyme immunoassays (EIAs) and Merck glycoprotein EIA (gpEIA). The TRFIA had equivalent sensitivity (97.8%) and high specificity (93.5%) in relation to gpEIA. A commercial (Behring) EIA compared favorably with TRFIA in terms of sensitivity (98.4%) but had lower specificity (80.7%). Another commercial EIA (Diamedix) had high specificity (97.1%) but low sensitivity (76.4%) compared to TRFIA if equivocal test results were treated as negative for VZV antibody. A novel feature of the TRFIA was that the cutoff was generated using population mixture modeling and was expressed in mIU/ml, as the assay was calibrated using the British standard VZV antibody.

DIRECT MEASUREMENT OF ARGININE-VASOPRESSIN IN HUMAN SERUM WITHOUT EXTRACTION PROCEDURE

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S130 ◽  
Author(s):  
H. Wagner ◽  
V. Maier ◽  
H.-J. Herrmann ◽  
H. E. Franz
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Arginine Vasopressin ◽  
Extraction Procedure
Sign in / Sign up

Export Citation Format

Share Document