Steady State and Time-resolved Fluorescence Investigation of the Specific Binding of Two Chlorin Derivatives with Human Serum Albumin

2007 ◽  
Vol 111 (35) ◽  
pp. 10557-10562 ◽  
Author(s):  
Sunita Patel ◽  
Anindya Datta
Keyword(s):  
Steady State ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Specific Binding ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals Unfolding of Acrylodan-Labeled Human Serum Albumin Probed by Steady-State and Time-Resolved Fluorescence Methods

1998 ◽  
Vol 75 (2) ◽  
pp. 1084-1096 ◽  
Author(s):  
Kulwinder Flora ◽  
John D. Brennan ◽  
Gary A. Baker ◽  
Meagan A. Doody ◽  
Frank V. Bright
Keyword(s):  
Steady State ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Methods

Glycation of Human Serum Albumin by DL-Glyceraldehyde: A Fluorescence Quenching Study

1997 ◽  
Vol 62 (11) ◽  
pp. 1815-1820 ◽  
Author(s):  
Miroslav Štěpánek ◽  
Zdeněk Pavlíček
Keyword(s):  
Steady State ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescence Decay ◽  
Fluorescence Lifetimes ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Trp Fluorescence ◽  
Quenching Efficiency

Process of glycation of human serum albumin (HSA) by DL-glyceraldehyde was studied using steady-state and time-resolved fluorescence spectroscopy in the course of 100 h. During this period, measurements of steady-state tryptophan (Trp) and non-tryptophan (non-Trp) fluorescence of the glycated HSA were carried out, together with measurement of the non-Trp fluorescence decay and steady-state quenching using potassium iodide as a quencher. Observed changes in both Trp and non-Trp fluorescence intensity, as well as changes in non-Trp fluorescence lifetimes and quenching efficiency are explained with respect to a probable mechanism of glycation.

Interaction of human serum albumin with liposomes of saturated and unsaturated lipids with different phase transition temperatures: a spectroscopic investigation by membrane probe PRODAN

RSC Advances ◽  
2014 ◽  
Vol 4 (28) ◽  
pp. 14335-14347 ◽  
Author(s):  
Raina Thakur ◽  
Anupam Das ◽  
Anjan Chakraborty
Keyword(s):  
Phase Transition ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Unsaturated Lipids ◽  
Membrane Probe ◽  
Phase Transition Temperatures

The interaction of human serum albumin (HSA) with liposomes made of saturated and unsaturated phosphocholines has been studied using circular dichroism (CD), steady state and time resolved fluorescence spectroscopic techniques.

Time-resolved fluorescence studies on site-directed mutants of human serum albumin

FEBS Letters ◽  
1997 ◽  
Vol 408 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Michael K Helms ◽  
Charles E Petersen ◽  
Nadhipuram V Bhagavan ◽  
David M Jameson
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Fluorescence Studies
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