Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy dispersive x-ray fluorescence. 1: Technique and determination of the normal reference interval.

1985 ◽  
Vol 31 (4) ◽  
pp. 551-555 ◽  
Author(s):  
E A Maier ◽  
F Rastegar ◽  
R Heimburger ◽  
C Ruch ◽  
A Pelletier ◽  
...  
Keyword(s):  
Trace Elements ◽  
Analytical Procedure ◽  
Reference Interval ◽  
Lavage Fluid ◽  
Energy Dispersive ◽  
Polypropylene Film ◽  
Internal Standards ◽  
X Ray ◽  
Large Numbers

Abstract We have applied energy dispersive x-ray fluorescence to the analysis of broncho-alveolar lavage fluids, to determine the concentration of several elements simultaneously with manipulation of the sample minimized. A 20-microL sample of a homogenized lavage fluid, to which two internal standards (containing Y and V) have been added, is deposited on a polypropylene film, 4 microns in thickness, and analyzed by x-ray fluorescence. We report the analytical procedure, the detection limits, and the reproducibility. The normal concentration range for trace elements is reported for a selected population. The simplicity and rapidity of the method, and the possibility of automating measurements, make this procedure suitable for screening large numbers of lavage specimens and to establish the diagnosis of some pneumoconioses.

Simultaneous determination of trace elements in serum by energy-dispersive x-ray fluorescence spectrometry.

1984 ◽  
Vol 30 (8) ◽  
pp. 1300-1303 ◽  
Author(s):  
F Rastegar ◽  
E A Maier ◽  
R Heimburger ◽  
C Christophe ◽  
C Ruch ◽  
...  
Keyword(s):  
Analytical Procedure ◽  
Internal Standard ◽  
Detection Limits ◽  
The Other ◽  
Energy Dispersive ◽  
Statistical Distributions ◽  
Polypropylene Film ◽  
X Ray ◽  
Large Numbers

Abstract Energy-dispersive x-ray fluorescence is applied in the analysis of human serum to determine the concentrations of several elements simultaneously with minimal manipulation of the sample. The analytical procedure has been developed with standard sera, and standardization, detection limits, and reproducibility have been established. A 50-microL sample of diluted serum, to which an internal standard has been added, is deposited on a thin (4-microns thick) polypropylene film and analyzed by x-ray fluorescence. We report the statistical distributions of the concentrations of Fe, Cu, Zn, and Br obtained in the population (103 samples) studied, and report detection limits for the other 22 elements studied. The simplicity of the method, the high throughput, and the possibility of automating the measurements make this procedure suitable for screening large numbers of sera.

Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy-dispersive x-ray fluorescence. 3. Routine analysis.

1987 ◽  
Vol 33 (12) ◽  
pp. 2234-2239 ◽  
Author(s):  
E A Maier ◽  
A Dietemann-Molard ◽  
F Rastegar ◽  
R Heimburger ◽  
C Ruch ◽  
...  
Keyword(s):  
Trace Elements ◽  
Occupational Exposure ◽  
Bronchoalveolar Lavage ◽  
Normal Values ◽  
Energy Dispersive ◽  
Fluorescence Technique ◽  
X Ray ◽  
High Concentrations ◽  
Number Of Cells

Abstract We applied the energy-dispersive x-ray fluorescence technique to determination of trace elements in human bronchoalveolar lavage fluids. Our analysis of more than 200 samples allowed us to determine normal reference values, to be used in characterizing occupational exposure. These values are expressed both in nanograms per 1000 cells (of all kinds) and nanograms per 1000 macrophages to correlate lavage efficiency and dust content of the alveoli. The result expressed in milligrams per liter is not sufficient, because some healthy volunteers showed high concentrations of iron but normal values when expressed vs the number of cells. Some examples of abnormal compositions of broncho-alveolar lavages are reported and the fully automated spectrometer developed for clinical and biological investigations is described.

Energy dispersive x-ray fluorescence spectrometric determination of trace elements in oil samples

1978 ◽  
Vol 50 (7) ◽  
pp. 899-903 ◽  
Author(s):  
Hideo. Kubo ◽  
Robert. Bernthal ◽  
Thomas R. Wildeman
Keyword(s):  
Trace Elements ◽  
Energy Dispersive ◽  
X Ray

Matrix corrections for quantitative determination of trace elements in biological samples using energy-dispersive X-ray fluorescence spectrometry

1989 ◽  
Vol 18 (5) ◽  
pp. 207-210 ◽  
Author(s):  
Sudhir Kumar ◽  
Surinder Singh ◽  
D. Mehta ◽  
M. L. Garg ◽  
P. C. Mangal ◽  
...  
Keyword(s):  
Trace Elements ◽  
Quantitative Determination ◽  
Biological Samples ◽  
Energy Dispersive ◽  
Fluorescence Spectrometry ◽  
X Ray
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