Determination of trace elements in organic specimens by energy-dispersive x-ray fluorescence using a fundamental parameters method

Abstract We have applied energy dispersive x-ray fluorescence to the analysis of broncho-alveolar lavage fluids, to determine the concentration of several elements simultaneously with manipulation of the sample minimized. A 20-microL sample of a homogenized lavage fluid, to which two internal standards (containing Y and V) have been added, is deposited on a polypropylene film, 4 microns in thickness, and analyzed by x-ray fluorescence. We report the analytical procedure, the detection limits, and the reproducibility. The normal concentration range for trace elements is reported for a selected population. The simplicity and rapidity of the method, and the possibility of automating measurements, make this procedure suitable for screening large numbers of lavage specimens and to establish the diagnosis of some pneumoconioses.

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Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy-dispersive x-ray fluorescence. 3. Routine analysis.

Clinical Chemistry ◽

10.1093/clinchem/33.12.2234 ◽

1987 ◽

Vol 33 (12) ◽

pp. 2234-2239 ◽

Cited By ~ 9

Author(s):

E A Maier ◽

A Dietemann-Molard ◽

F Rastegar ◽

R Heimburger ◽

C Ruch ◽

...

Keyword(s):

Trace Elements ◽

Occupational Exposure ◽

Bronchoalveolar Lavage ◽

Normal Values ◽

Energy Dispersive ◽

Fluorescence Technique ◽

X Ray ◽

High Concentrations ◽

Number Of Cells

Abstract We applied the energy-dispersive x-ray fluorescence technique to determination of trace elements in human bronchoalveolar lavage fluids. Our analysis of more than 200 samples allowed us to determine normal reference values, to be used in characterizing occupational exposure. These values are expressed both in nanograms per 1000 cells (of all kinds) and nanograms per 1000 macrophages to correlate lavage efficiency and dust content of the alveoli. The result expressed in milligrams per liter is not sufficient, because some healthy volunteers showed high concentrations of iron but normal values when expressed vs the number of cells. Some examples of abnormal compositions of broncho-alveolar lavages are reported and the fully automated spectrometer developed for clinical and biological investigations is described.

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Determination of trace elements in urban airborne particulates (PM10) using energy dispersive X-ray fluorescence (EDXRF) spectroscopy

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v18i4.8 ◽

2015 ◽

Vol 18 (4) ◽

pp. 609

Author(s):

NI Elom ◽

ME Deary ◽

J Dean

Keyword(s):

Trace Elements ◽

Energy Dispersive ◽

Airborne Particulates ◽

X Ray

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The Determination of Trace Elements Release from Dental Cements in Artificial Saliva by Energy Dispersive X‐ray Fluorescence Spectrometry

Analytical Letters ◽

10.1080/00032710701585560 ◽

2007 ◽

Vol 40 (13) ◽

pp. 2476-2484 ◽

Cited By ~ 3

Author(s):

N. Ekinci ◽

F. Bayindir ◽

Y. Z. Bayindir ◽

R. Ekinci

Keyword(s):

Trace Elements ◽

Artificial Saliva ◽

Energy Dispersive ◽

Fluorescence Spectrometry ◽

X Ray

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Quantitative analysis by energy dispersive X-ray fluorescence by the transmission method applied to geological samples

Scientia Agricola ◽

10.1590/s0103-90161994000200001 ◽

1994 ◽

Vol 51 (2) ◽

pp. 197-206 ◽

Cited By ~ 11

Author(s):

S.M. Simabuco ◽

V.F. Nascimento Filho

Keyword(s):

Atomic Number ◽

Energy Dispersive ◽

X Rays ◽

Geological Samples ◽

Transmission Method ◽

Absorption Effect ◽

X Ray ◽

Fundamental Parameters ◽

Absorption Correction

Three certified samples of different matrices (Soil-5, SL-1/IAEA and SARM-4/SABS) were quantitatively analysed by energy dispersive X-ray fluorescence with radioisotopic excitation. The observed errors were about 10-20% for the majority of the elements and less than 10% for Fe and Zn in the Soil-5, Mn in SL-1, and Ti, Fe and Zn in SARM-4 samples. Annular radioactive sources of Fe-55 and Cd-109 were utilized for the excitation of elements while a Si(Li) semiconductor detector coupled to a multichannel emulation card inserted in a microcomputer was used for the detection of the characteristic X-rays. The fundamental parameters method was used for the determination of elemental sensitivities and the irradiator or transmission method for the correction of the absorption effect of characteristic X-rays of elements on the range of atomic number 22 to 42 (Ti to Mo) and excitation with Cd-109. For elements in the range of atomic number 13 to 23 (Al to V) the irradiator method cannot be applied since samples are not transparent for the incident and emergent X-rays. In order to perform the absorption correction for this range of atomic number excited with Fe-55 source, another method was developed based on the experimental value of the absorption coefficients, associated with absorption edges of the elements.

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