Urinary alanine aminopeptidase assay improved as result of multivariate response-surface analysis.

1988 ◽  
Vol 34 (5) ◽  
pp. 954-957 ◽  
Author(s):  
C Flandrois ◽  
C Lahet ◽  
D Feldmann ◽  
J M Gabastou ◽  
A Gonnon ◽  
...  
Keyword(s):  
Response Surface ◽  
Surface Analysis ◽  
Response Surface Analysis ◽  
Tris Buffer ◽  
Multivariate Response ◽  
Multivariate Study ◽  
Alanine Aminopeptidase

Abstract Optimization of determination of alanine aminopeptidase in urine by univariate study led to a method involving pretreatment of urine with Sephadex G50. Re-examination of the optimization by multivariate study led us to recommend higher optimal concentrations: 5.8 mmol/L for the substrate and 300 mmol/L for the Tris buffer. Under these new conditions, pretreatment of urine was no longer necessary and the assay could be completely automated.

Development Of A Fully Automated Functional Fibrinogen Assay

1981 ◽  
Author(s):  
G E Siefring ◽  
J A Wehrly
Keyword(s):  
Response Surface ◽  
Surface Analysis ◽  
Heparin Therapy ◽  
Second Step ◽  
Fibrinogen Concentration ◽  
Correlation Studies ◽  
Multivariate Response ◽  
Automated Method ◽  
Automated Determination

A fully automated method for the determination of functional fibrinogen has been developed for the Du Pont ‘aca’. The basis of the assay is the measurement of turbidity formation upon the addition of thrombin to the test plasma. The method can measure fibrinogen in the plasma of patients on heparin therapy and provides a stat result in eight minutes.The assay of fibrinogen is accomplished in a two-stage sequence. First, clot enhancing agents and a heparin neutralizing compound are added. Thrombin is added in the second step to catalyze fibrin formation, the rate of turbidity change being proportional to the fibrinogen concentration. Optimization of the assay under ‘aca’ conditions was accomplished through the use of multivariate response surface analysis. The analytical performance of the method was judged to be acceptable based on precision, interference, and correlation studies. The range of the method is 40-1000 mg/dl fibrinogen. This ‘aca’ method represents the first fully automated determination of functional fibrinogen.

Developing Optimum Drug Regimens

1976 ◽  
Vol 4 (5) ◽  
pp. 277-280
Author(s):  
Michael S Carey ◽  
Robert W Noyes ◽  
Samuel A Pasquale ◽  
John J Frey ◽  
John S Lawson
Keyword(s):  
Response Surface ◽  
Surface Analysis ◽  
Dose Response ◽  
Response Surface Analysis ◽  
Human Experimentation ◽  
Substantial Saving ◽  
Drug Regimens

In Clinical pharmacological trials the determination of the optimum regimen for a drug using the technique of response surface analysis is proposed as being more advantageous than traditional analyses of dose response relationships. Major considerations in favour of this technique are that the optimum regimen can be identified with a minimum of human experimentation, and unacceptable regimens are eliminated from the study early, at a substantial saving of time and money.

scholarly journals Response surface analysis of nozzle parameters at supersonic flow through microjets

2021 ◽  
pp. 1-16
Author(s):  
Turki Al-Khalifah ◽  
Abdul Aabid ◽  
Sher Afghan Khan ◽  
Muhammad Hanafi Bin Azami ◽  
Muneer Baig
Keyword(s):  
Supersonic Flow ◽  
Response Surface ◽  
Surface Analysis ◽  
Response Surface Analysis ◽  
Flow Through
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