Highly Sensitive Chemiluminescent Enzyme Immunoassay with Gelatin-Coated Ferrite Solid Phase

1994 ◽  
Vol 40 (9) ◽  
pp. 1830-1831 ◽  
Author(s):  
Mitsuo Isomura ◽  
Masayoshi Ueno ◽  
Kazuya Shimada ◽  
Hiroyuki Kogaki ◽  
Yoshihiro Ashihara
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Highly Sensitive ◽  
Chemiluminescent Enzyme Immunoassay

Specific enzyme immunoassay for human chorionic gonadotrophin

1981 ◽  
Vol 97 (4) ◽  
pp. 562-568 ◽  
Author(s):  
Tatsuhiro Sekiya ◽  
Yoshihito Furuhashi ◽  
Setsuko Goto ◽  
Shigeaki Kaseki ◽  
Yutaka Tomoda ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Serum Samples ◽  
Coefficients Of Variation ◽  
Highly Sensitive ◽  
Assay Tube ◽  
Sandwich Type ◽  
Serial Samples

Abstract. A sandwich-type enzyme immunoassay system specific for human chorionic gonadotrophin (hCG) was prepared with the antibody Fab'-β-D-galactosidase complex and the antibody F(ab')2-immobilized silicone rubber solid phase by using a purified antibody to β subunit of hCG (hCGβ). The assay system cross-reacted less than 4% with human luteinizing hormone (hLH) and human follicular stimulating hormone (hFSH), and proved to be highly sensitive with hCG measurable at levels as low as 0.3 mIU per assay tube. Using 50 μl of serum sample, 6–600 mIU/ml of hCG in serum could be determined specifically with the same degree of precision as in radioimmunoassay but without sample interference with the assay. The coefficients of variation within-run and between-run were 8.6–8.9%, and 4.9– 10.7%, respectively. Values obtained with the enzyme immunoassay correlated well with those of radioimmunoassay ([unk] = 0.98, slope = 0.94, y-intercept = 10.2 mIU/ml for 75 serum samples). Results of the immunoassay of hCG levels in serial samples of serum from healthy women and patients with choriocarcinoma show that this method is useful in the clinical diagnosis of trophoblastic disease.

scholarly journals Evaluation of Solid-Phase Chemiluminescent Enzyme Immunoassay, Enzyme-Linked Immunosorbent Assay, and Latex Agglutination Tests for Screening Toxoplasma IgG in Samples Obtained from Cats and Pigs

2000 ◽  
Vol 12 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Ashok K. Singh
Keyword(s):  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Solid Phase ◽  
Latex Agglutination ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Serum Samples ◽  
Positive Results ◽  
Chemiluminescent Enzyme Immunoassay

Serum samples from cats and pigs were analyzed by the solid-phase chemiluminescent enzyme immunoassay (SPCEI), enzyme-linked immunosorbent assay (ELISA), and indirect latex agglutination (ILA) methods. The SPCEI and ILA methods accurately analyzed Toxoplasma IgG (T-IgG) in both clinical and spiked samples from pigs and cats. The ELISA method accurately analyzed T-IgG in spiked samples from cats and pigs or clinical samples from pigs, but it did not accurately analyze T-IgG in clinical samples from cats. The antibody used in the ELISA kit did not cross-react with cat T-IgG. The SPCEI method that uses a stand-alone automated analyzer provided quantitative analysis, whereas the ELISA and ILA methods provided qualitative or, at best, semiquantitative analysis of T-IgG. The SPCEI and ELISA methods were rapid (60–90 minutes for 30 samples), whereas the ILA method required 13–15 hours for 30 samples. Although the three methods accurately distinguished positive from negative samples, the ILA method yielded many weakly positive results that were not confirmed by either the ELISA or SPCEI method. Thus, the indirect agglutination tests may give nonspecific responses at lower T-IgG concentrations.

scholarly journals Excess EDTA interferes with cortisol measurement using a solid-phase, chemiluminescent enzyme immunoassay

2018 ◽  
Vol 30 (3) ◽  
pp. 438-441 ◽  
Author(s):  
Robert J. Kemppainen ◽  
Ellen N. Behrend ◽  
Stephanie F. Carter ◽  
Janeva E. Cole
Keyword(s):  
Alkaline Phosphatase ◽  
Plasma Concentration ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Serum Samples ◽  
Edta Plasma ◽  
Edta Concentration ◽  
Canine Serum ◽  
Chemiluminescent Enzyme Immunoassay

Hormone assays that use a solid-phase, automated, chemiluminescent enzyme immunoassay (CEIA) with an alkaline phosphatase–tagged hormone or antibody as a reporter are performed on serum or EDTA plasma in our laboratory. CEIA cortisol results appeared to increase in the presence of excess EDTA. We investigated the effect of the addition of different amounts of EDTA on cortisol concentrations in pooled canine serum samples. The recommended EDTA plasma concentration of 4.1 mmol/L (1.8 mg/mL) did not alter cortisol concentrations when added to serum pools; however, the addition of ≥5.1 mmol/L (2.25 mg/mL) of EDTA increased apparent concentrations of cortisol. Supplementation of serum samples with MgCl2 to 5 mmol/L reversed the effect of EDTA up to a concentration of ~8.1 mmol/L (3.6 mg/mL). Our findings show that CEIA cortisol results on EDTA plasma can be artificially increased if the EDTA concentration exceeds 5.1 mmol/L.

Highly sensitive solid-phase enzyme immunoassay for terminal deoxynucleotidyl transferase

1982 ◽  
Vol 126 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Tsuguhiro Kaneda ◽  
Saiko Kuroda ◽  
Yutaka Hirota ◽  
Kanefusa Kato
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Highly Sensitive

scholarly journals Evaluation of Three Commercial Serological Tests with Different Methodologies To Assess Helicobacter pyloriInfection

1999 ◽  
Vol 37 (12) ◽  
pp. 4150-4152 ◽  
Author(s):  
A. van der Ende ◽  
R. W. M. van der Hulst ◽  
P. Roorda ◽  
G. N. J. Tytgat ◽  
J. Dankert
Keyword(s):  
Helicobacter Pylori ◽  
Enzyme Immunoassay ◽  
Immunosorbent Assay ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
H Pylori ◽  
Chemiluminescent Enzyme Immunoassay

The sera of 142 Helicobacter pylori-positive and 32H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.

A rapid and highly sensitive solid-phase enzyme immunoassay specific for human fibronectin using a characterized monoclonal antibody

1984 ◽  
Vol 72 (1) ◽  
pp. 145-156 ◽  
Author(s):  
Eeva-Marjatta Salonen ◽  
Tapio Vartio ◽  
Vincenzo Miggiano ◽  
Christian Stähli ◽  
Bela Tacács ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Highly Sensitive ◽  
Human Fibronectin
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