scholarly journals Modification of Fully Automated Total Iron-binding Capacity (TIBC) Assay in Serum and Comparison with Dimension TIBC Method

2002 ◽  
Vol 48 (9) ◽  
pp. 1565-1570 ◽  
Author(s):  
Hachiro Yamanishi ◽  
Shigeru Iyama ◽  
Yoshihisa Yamaguchi ◽  
Yuzuru Kanakura ◽  
Yoshinori Iwatani
Keyword(s):  
Binding Capacity ◽  
Clinical Chemistry ◽  
Transferrin Saturation ◽  
Original Method ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Colored Complex ◽  
Modified Method ◽  
Iron Binding Capacity

Abstract Background: We previously reported the development of a fully automated assay for total iron-binding capacity (TIBC) in serum, using a multipurpose automated analyzer. However, this method requires four different reagents and is thus useful only with a limited number of available analyzers. We simplified our original assay and compared the analytical performance of the modified method with that of a commercial, fully automated TIBC assay (Dimension® TIBC assay). Methods: We simplified our original method to require only three reagents. Calibration was also altered and was performed with human transferrin standard solutions. An advantage of this method is that it does not require separation of excess unbound iron after the first step of transferrin saturation. Unbound iron is eliminated by formation of a complex with the chromogenic reagent ferrozine in the second step. Iron dissociated from transferrin by acidic pH reacts with ferrozine to form a colored complex in the final step, and the increase in absorbance at 570/660 nm is directly proportional to the TIBC measured. TIBC values were determined for 49 healthy individuals and 148 patients with this modified TIBC assay and with a commercial, fully automated TIBC method (Dimension clinical chemistry system), and calculation of TIBC based on the sum of the serum iron and unsaturated iron-binding capacity was performed for 97 patients. Results: The within-run CVs for the modified TIBC assay and the Dimension TIBC assay were <4.8% and <2.4%, and the between-run CVs were 1.2% and 1.7%, respectively. The dilution curves were linear for TIBC values up to at least 180 μmol/L with both methods. TIBC values obtained by our method were linearly correlated with serum transferrin concentrations (r = 0.984; Sy|x = 3.18 μmol/L; P <0.001). The correlation between the values obtained with the present method (y) and those obtained with the Dimension TIBC method (x) was y = 1.04x + 1.19 μmol/L (r = 0.985; Sy|x = 2.47 μmol/L), and with the calculation method (x) was y = 1.18x + 2.62 μmol/L (r = 0.976; Sy|x = 3.27 μmol/L). Conclusions: Our modified, fully automated TIBC assay performed similarly to the Dimension TIBC assay and is adaptable for use with many multipurpose automated analyzers.

Serum iron and total iron-binding capacity compared with serum ferritin in assessment of iron deficiency.

1981 ◽  
Vol 27 (2) ◽  
pp. 276-279 ◽  
Author(s):  
F Peter ◽  
S Wang
Keyword(s):  
Iron Deficiency ◽  
Serum Ferritin ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Deficient ◽  
Iron Binding Capacity

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

scholarly journals Gambaran status besi pada pasien penyakit ginjal kronik stadium 5 dengan anemia yang menjalani hemodialisis reguler

e-CliniC ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Bryan J. Silaban ◽  
Cerelia Sugeng ◽  
Bradley J. Waleleng
Keyword(s):  
End Stage Renal Disease ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Stage Renal Disease ◽  
End Stage ◽  
Iron Binding Capacity

Abstract: Chronic kidney disease (CKD) is a pathological condition with a variety of etiology, resulting in progressively decreased renal function which is often ended with kidney failure. Chronic kidney disease has a global prevalence of 800 per million of population and the incidence of end-stage renal disease ranges from 150 to 200 per million of population. Complications often occur at the end-stage renal disease inter alia anemia with a rate of 80-90%. This study was aimed to obtain the profile of stage-5 CKD patients with anemia and regular hemodialysis was performed on them. This was a descriptive-retrospective study using data of medical records at Prof. Dr. R. D. Kandou General Hospital from January 2015 to October 2016. There were six observed variables as follows: age, gender, serum iron (SI), total iron binding capacity (TIBC), transferrin saturation, and ferritin. The results showed that of the 48 CKD patients with anemia, there were 24 males and 24 females. The majority were aged 60-69 years (33%) and had normal SI level in 30 patients (67%); decreased TIBC in 35 patients (75%); normal transferrin saturation in 26 patients (54%); and increased ferritin level in 38 patients (81%). Conclusion: Majority of the patients were 60-69 years old and had normal level of SI, decreased TIBC, normal transferrin saturation, and increased levels of ferritin. There was no difference in case number of both sexes.Keywords: serum iron, total iron binding capacity, transferrin saturation, ferritin Abstrak: Penyakit Ginjal Kronik (PGK) adalah suatu keadaan patologis dengan etiologi yang beragam, terjadi penurunan fungsi ginjal secara progresif, dan biasanya berakhir dengan gagal ginjal. Penyakit ginjal kronik merupakan salah satu masalah kesehatan di dunia dengan prevalensi 800 per juta populasi dan insidensi end-stage renal disease (ESRD) 150-200 per juta populasi di dunia. Komplikasi sering terjadi pada PGK stadium akhir antara lain anemia dengan persentase mencapai 80-90%. Penelitian ini bertujuan untuk mendapatkan profil pasien PGK stadium 5 dengan anemia dan menjalani hemodialisis reglular. Jenis penelitian ialah deskriptif-retrospektif menggunakan data sekunder dari catatan rekam medik periode Januari 2015 – Oktober 2016 di RSUP Prof. Dr. R. D. Kandou Manado dengan enam variabel penelitian, yaitu usia, jenis kelamin, serum iron (SI), total iron binding capacity (TIBC), saturasi transferin, dan feritin. Dari 48 data rekam medik pasien yang memenuhi kriteria inklusi ditemukan bahwa mayoritas pasien berumur 60-69 tahun (33%), laki-laki maupun perempuan berjumlah sama, mayoritas pasien memiliki kadar SI normal berjumlah 30 pasien (67%), kadar TIBC menurun berjumlah 35 pasien (75%), saturasi transferin normal berjumlah 26 pasien (54%), dan kadar feritin meningkat berjumlah 38 pasien (81%). Simpulan: Mayoritas pasien PGK stadium 5 yang menjalani hemodialisis reguler berusia 60-69 tahun, laki-laki dan perempuan berjumlah sama, serta memiliki kadar SI normal, penurunan TIBC, saturasi transferin normal, dan peningkatan kadar feritin. Kata kunci: serum iron, total iron binding capacity, saturasi transferin, feritin

scholarly journals Immunoturbidimetric determination of serum transferrin on a Kone Progress autoanalyser

1989 ◽  
Vol 11 (1) ◽  
pp. 40-41 ◽  
Author(s):  
Luc Cynober ◽  
Jacques Le Boucher ◽  
Jacqueline Giboudeau
Keyword(s):  
Binding Capacity ◽  
Clinical Chemistry ◽  
Total Iron ◽  
Radial Immunodiffusion ◽  
Serum Transferrin ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
French Society ◽  
Iron Binding Capacity

The Kone Progress, a multiparametric discrete analyser, was used to determine serum transferrin with a kit supplied by Kone. Assays recommended by the French Society of Clinical Chemistry were performed in order to assess the suitability of the test. Repeatability was assessed using serum pools with low (L), medium (M) and high (H) concentrations of transferrin. The coeffcients of variation (CV) were 5.4, 3.2 and 2.0% respectively for 30 determinations (within-batch). Reproducibility on 15 consecutive days (between-batch) was also satisfactory (CV for L = 7.3%, M = 6.3% and H = 3.8%). There was no serum-to-serum contamination. Results correlated closely with those obtained using radial immunodiffusion (RID) (r = 0.942) and total iron-binding capacity (r = 0.954)for 90 determinations.Transferrin measurement by immunoturbidimetry on the Kone Progress emerges as a well-suited, rapid and inexpensive alternative to other time-consuming (RID) and sophisticated (laser immunonephelemeter) techniques.

scholarly journals Assessment of Pro Hepcidin and Related with Iron Profile on Hemodialysis Men Patients

2016 ◽  
Vol 13 (1) ◽  
pp. 74-81
Author(s):  
Baghdad Science Journal
Keyword(s):  
Renal Failure ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Extracellular Fluid ◽  
Total Iron ◽  
Iron Store ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Binding Capacity ◽  
Iron Profile

Patients with renal failure in the final stages undergo the treatment by hemodialysis. Hemodialysis is used to reinstate the intracellular and extracellular fluid environment, by propagation of molecules in solution through a semipermeable membrane along an electrochemical concentration gradient. Blood catching in the dialysis machine and the recurrent phlebotomy may lead to losing about 1-3 g of iron per year. Prohepcidin hormone is an acute phase protein (type II) that plays a major role in the systemic iron irregularities as it is a mediator of anemia in inflammation and regulator of iron metabolism. This study aims to evaluate the effect of hemodialysis on iron hemostasis and its relationship with prohepcidin as an inflammatory marker. This study includes forty four adult male patients with end-stage renal failure (in pre and post –treated) by means of chronic hemodialysis-HD with mean age (53.27 ± 13.76 years). The following biochemical investigations have been studied: Prohepcidin, Iron, Ferritin, Transferrin, Total Iron-Binding Capacity (TIBC), The Unsaturated Total Iron Binding Capacity (UIBC), and transferrin saturation (TAST).Decrement of Prohepcidin level on hemodialysis patients in post dialysis with non-significantly compared to pre dialysis, while iron and ferritin was increment in post treated than pre- treated with non-significantly.Hemodialysis affects Prohepcidin levels as it was long duration and Glomerular Filtration rate GFR (cock croft equation) and prohepcidin level affect the iron profile related with the iron store depletion.

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