An Extremely Rare Malignant DabsKa Tumor in Right Atria for A Premature Neonate

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

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Transcriptomic uniqueness and commonality of the ion channels and transporters in the four heart chambers

Scientific Reports ◽

10.1038/s41598-021-82383-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sanda Iacobas ◽

Bogdan Amuzescu ◽

Dumitru A. Iacobas

Keyword(s):

Ion Channels ◽

Cycle Phase ◽

Functional Roles ◽

Expression Control ◽

Gene Expression Control ◽

Left And Right ◽

The Right ◽

Phase Out ◽

Right Atria ◽

Agilent Microarrays

AbstractMyocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcriptome dependence on the estrous cycle phase. Out of the quantified 16,886 unigenes, 15.76% on the left side and 16.5% on the right side exhibited differential expression between the atrium and the ventricle, while 5.8% of genes were differently expressed between the two atria and only 1.2% between the two ventricles. The study revealed also chamber differences in gene expression control and coordination. We analyzed ion channels and transporters, and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/gluconeogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 oscillates in phase with all 27 quantified binding partners in the left ventricle, the percentage of in-phase oscillating partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis indicated high interventricular synchrony of the ion channels expressions and the substantially lower synchrony between the two atria and between the atrium and the ventricle from the same side.

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Cholinesterase activity of left and right atria of the rabbit's heart

The Journal of Physiology ◽

10.1113/jphysiol.1954.sp005231 ◽

1954 ◽

Vol 126 (3) ◽

pp. 623-626 ◽

Cited By ~ 5

Author(s):

Sheila Briscoe

Keyword(s):

Cholinesterase Activity ◽

Left And Right ◽

Right Atria

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A Lethal Case ofSphingomonas paucimobilisBacteremia in an Immunocompromised Patient

Case Reports in Infectious Diseases ◽

10.1155/2016/3294639 ◽

2016 ◽

Vol 2016 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Nata Pratama Hardjo Lugito ◽

Cucunawangsih ◽

Andree Kurniawan

Keyword(s):

Septic Shock ◽

Foot Ulcer ◽

Diabetic Foot Ulcer ◽

Severe Infection ◽

Venous Catheter ◽

Multidrug Resistant ◽

Premature Neonate ◽

Hospital Environment ◽

Indwelling Catheters ◽

Stage Renal Disease

Sphingomonas paucimobilisis a yellow-pigmented, glucose nonfermenting, aerobic, Gram negative bacillus of low pathogenicity. This organism was found in the implantation of indwelling catheters, sterile intravenous fluid, or contaminated hospital environment such as tap and distilled water, nebulizer, ventilator, and hemodialysis device. A 55-year-old female was hospitalized for diabetic foot ulcer in the presence of multiple comorbidities: diabetes mellitus, colonic tuberculosis, end-stage renal disease, and indwelling catheters for central venous catheter and hemodialysis. The patient passed away on the 44th day of admission due to septic shock. The organism found on blood culture on the 29th day of admission was multidrug resistantS. paucimobilis. Severe infection and septic shock due toS. paucimobilishave been reported particularly in immunocompromised patients, but there has been only one reported case of death in a premature neonate with septic shock. This is the first reported lethal case ofS. paucimobilisbacteremia in an adult patient.

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