scholarly journals Sequencing, Assembly and Annotation of the Whole-Insect Genome of Lymantria dispar dispar, the European Gypsy Moth

2021 ◽  
Author(s):  
Michael E Sparks ◽  
Francois Olivier Hebert ◽  
J Spencer Johnston ◽  
Richard Hamelin ◽  
Michel Cusson ◽  
...  
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Gc Content ◽  
Urban Trees ◽  
Genome Sequences ◽  
Insect Genome ◽  
Genetic Features ◽  
Asian Gypsy Moth ◽  
Long Interspersed Nuclear Elements ◽  
Reference Sequences

Abstract The European gypsy moth, Lymantria dispar dispar (LDD), is an invasive insect and a threat to urban trees, forests and forest-related industries in North America. For use as a comparator with a previously published genome based on the LD652 pupal ovary-derived cell line, as well as whole-insect genome sequences obtained from the Asian gypsy moth subspecies L. dispar asiatica and L. dispar japonica, the whole-insect LDD genome was sequenced, assembled and annotated. The resulting assembly was 998 Mb in size, with a contig N50 of 662 Kb and GC content of 38.8%. Long interspersed nuclear elements (LINEs) constitute 25.4% of the whole-insect genome, and a total of 11,901 genes predicted by automated gene finding encoded proteins exhibiting homology with reference sequences in the NCBI NR and/or UniProtKB databases at the most stringent similarity cutoff level (i.e., the gold tier). These results will be especially useful in developing a better understanding of the biology and population genetics of L. dispar and the genetic features underlying Lepidoptera in general.

scholarly journals Gypsy moth genome provides insights into flight capability and virus–host interactions

2019 ◽  
Vol 116 (5) ◽  
pp. 1669-1678 ◽  
Author(s):  
Jing Zhang ◽  
Qian Cong ◽  
Emily A. Rex ◽  
Winnie Hallwachs ◽  
Daniel H. Janzen ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Draft Genome ◽  
Immunological Response ◽  
The United States ◽  
Muscle Adaptation ◽  
Phenotypic Differences ◽  
Plant Foliage ◽  
Asian Gypsy Moth

Since its accidental introduction to Massachusetts in the late 1800s, the European gypsy moth (EGM; Lymantria dispar dispar) has become a major defoliator in North American forests. However, in part because females are flightless, the spread of the EGM across the United States and Canada has been relatively slow over the past 150 years. In contrast, females of the Asian gypsy moth (AGM; Lymantria dispar asiatica) subspecies have fully developed wings and can fly, thereby posing a serious economic threat if populations are established in North America. To explore the genetic determinants of these phenotypic differences, we sequenced and annotated a draft genome of L. dispar and used it to identify genetic variation between EGM and AGM populations. The 865-Mb gypsy moth genome is the largest Lepidoptera genome sequenced to date and encodes ∼13,300 proteins. Gene ontology analyses of EGM and AGM samples revealed divergence between these populations in genes enriched for several gene ontology categories related to muscle adaptation, chemosensory communication, detoxification of food plant foliage, and immunity. These genetic differences likely contribute to variations in flight ability, chemical sensing, and pathogen interactions among EGM and AGM populations. Finally, we use our new genomic and transcriptomic tools to provide insights into genome-wide gene-expression changes of the gypsy moth after viral infection. Characterizing the immunological response of gypsy moths to virus infection may aid in the improvement of virus-based bioinsecticides currently used to control larval populations.

scholarly journals Transcriptomic and Metabolomic Analyses to Unravel Responses of Populus Simonii × P. Nigra to attack by Asian Gypsy moth Lymantria Dispar (Lepidoptera: Lymantriidae)

2020 ◽  
Author(s):  
Yuntong LV ◽  
Lili Sun ◽  
Jingjing Yin ◽  
Yanpeng Jia ◽  
Hui Du ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Molecular Mechanism ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Herbivorous Insects ◽  
Defensive Response ◽  
Mechanical Wounding ◽  
Populus Simonii ◽  
Asian Gypsy Moth ◽  
Key Genes

Abstract Background: Poplar is frequently attacked by herbivorous insects, including the Asian gypsy moth, Lymantria dispar. Here, we combined metabolomic and transcriptomic analysis to identify key genes and metabolites involved into the molecular mechanism of defensive enhancement against L. dispar herbivory on poplar. Results: The 3666 differentially expressed genes (DEGs, 1,799 up-regulated and 1,867 down-regulated) and 1,171 DEGs (695 up-regulated and 476 down-regulated) were specific in L. dispar herbivory and mechanical wounding, respectively. Moreover, the 9,108 and 7,656 ions were detected while 636 and 531 different ions were obtained using positive (pos) mode and negative (neg) mode, respectively. Among these ions, the 33 and 7 different ions were specific in L. dispar herbivory and mechanical wounding in pos mode while 46 and 4 different ions in L. dispar herbivory and mechanical wounding in neg mode, respectively. The 3,666 specific DEGs in L. dispar herbivory group were classified into phenylpropanoid and flavonoid secondary metabolism pathways by comprehensive networks between transcriptomes and metabolomes. Conclusions: The current findings greatly improve our understanding of the induced defensive response in poplar plants against L. dispar infestation, and will contribute to develop insect-resistance poplar varieties.

scholarly journals Impact of Temperature on Postdiapause and Diapause of the Asian Gypsy Moth,Lymantria dispar asiatica

2014 ◽  
Vol 14 (5) ◽  
pp. 1-9 ◽  
Author(s):  
Jing Wei ◽  
You-Qing Luo ◽  
Juan Shi ◽  
Dei-Peng Wang ◽  
Shao-Wei Shen
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Asian Gypsy Moth

scholarly journals Effects of methoxyfenozide on ecdysone receptor complex expression in larval Asian gypsy moth

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Shao-ping Wu ◽  
Li-li Sun ◽  
Hua Zhong ◽  
Shan-chun Yan ◽  
Hong-qu Wu ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Receptor Complex ◽  
Ecdysone Receptor ◽  
Insect Growth Regulators ◽  
Transcription Profiles ◽  
Asian Gypsy Moth ◽  
Complex Expression ◽  
Regulate Gene

AbstractAs a target of insect growth regulators, the ecdysone receptor complex binds ecdysone agonists to regulate gene transcription, development, and growth. To understand the response of Lymantria dispar to methoxyfenozide, we studied receptor complex transcription profiles following larval exposure to methoxyfenozide, and observed important variations amongst post-embryonic stages. After the exposure of 2nd and 3rd instar larvae to LC5 and LC20 methoxyfenozide for 72 h, the transcription levels of ecdysone receptor complex genes were mainly upregulated in 2nd instar larvae, but downregulated in 3rd instar larvae. These results provide expression characteristics of the ecdysone receptor complex in L. dispar larvae after methoxyfenozide exposure.

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