SEQUENCING STUDIES OF ICR-170 MUTAGENIC SPECIFICITY IN THE am (NADP-SPECIFIC GLUTAMATE DEHYDROGENASE) GENE OF NEUROSPORA CRASSA
Keyword(s):
Uv Light
◽
ABSTRACT The acridine half-mustard ICR-170-induced reversion of the mutant am15, which has a single base-pair deletion, at a frequency of between 9 and 28 × 10-6. In each of three classes of revertants, the mutagen had induced the insertion of a (see PDF) base pair at a (see PDF) site. The mutant am6, which has a single base pair insertion, is known to be revertible, with UV light, by deletion of a (see PDF) base pair at a (see PDF) site. This mutant reverted with ICR-170 at a frequency of 0.1 × 10-6. These results show that ICR-170 is able to induce addition frameshifts in Neurospora crassa within short, monotonous runs of G:C base pairs, but indicate a lack of deletion activity at such sequences.
2002 ◽
Vol 184
(20)
◽
pp. 5619-5624
◽
2004 ◽
Vol 72
(1)
◽
pp. 22-28
◽
Keyword(s):
2000 ◽
Vol 181
(6)
◽
pp. 2080-2082
◽
Keyword(s):
Keyword(s):
1998 ◽
Vol 20
(3)
◽
pp. 246-251
◽
Keyword(s):
1991 ◽
Vol 266
(35)
◽
pp. 23872-23877